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An Improved Tissue Culture And Transformation System For Switchgrass (Panicum Virgatum L.), Jason Neil Burris
An Improved Tissue Culture And Transformation System For Switchgrass (Panicum Virgatum L.), Jason Neil Burris
Masters Theses
Switchgrass (Panicum virgatum), a summer perennial grass native to North America, is currently being explored for its potential use in the production of biofuels. With these interests, genetic manipulation of switchgrass to produce plants that are easier to digest, have an increased resistance to diseases and stresses, and maintain viability longer in the field are required. Therefore, it was necessary to develop a reliable and efficient tissue culture system for the transformation of switchgrass. Current switchgrass tissue culture requires months for regeneration of transformants with relatively poor transformation efficiencies and are limited to derivatives of a single variety, Alamo. We …