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Full-Text Articles in Physiology
Acquisition Delays Affect Lymphocyte Subset Counts But Not Markers Of Exercise-Induced Apoptosis, Holly B. Kell, Guilherme Borges Pereira, Ramires Alsamir Tibana, Michael Jarrett, Mark A. Schafer, Jonato Prestes, Antonio Santo, James W. Navalta
Acquisition Delays Affect Lymphocyte Subset Counts But Not Markers Of Exercise-Induced Apoptosis, Holly B. Kell, Guilherme Borges Pereira, Ramires Alsamir Tibana, Michael Jarrett, Mark A. Schafer, Jonato Prestes, Antonio Santo, James W. Navalta
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 250-255, 2013. With the emergence of shared facilities, the possibility of a processing delay is increased as time on instrumentation becomes less available. The purpose of this investigation was the evaluate the effect of a 24h time delay on lymphocyte subset concentration, as well as for the apoptotic marker annexin V. Fourteen healthy individuals completed an incremental treadmill test to exhaustion, and blood samples were obtained before and after exercise. The samples were labeled in duplicate with mixtures consisting of flow cytometry staining buffer, the biomarker for early-phase cell death (annexin V), and …
Effects Of Two Weeks Of High-Intensity Interval Training (Hiit) On Monocyte Tlr2 And Tlr4 Expression In High Bmi Sedentary Men, Marta Child, Melanie Leggate, Michael Gleeson
Effects Of Two Weeks Of High-Intensity Interval Training (Hiit) On Monocyte Tlr2 And Tlr4 Expression In High Bmi Sedentary Men, Marta Child, Melanie Leggate, Michael Gleeson
International Journal of Exercise Science
International Journal of Exercise Science 6(1) : 81-90, 2013. Monocyte TLR expression has been shown to be reduced after a combination of aerobic and resistance exercise, but more studies considering the influences of different exercise intensities, type and duration on TLR expression are needed. Although there is an agreement about the importance of physical exercise, the minimal amount needed to improve health status is uncertain. Therefore, the aim of this study was to analyse the influence of 2 weeks of high-intensity intermittent exercise training on CD14+ monocyte TLR4 expression in a sedentary, high BMI population. As a secondary purpose, this …
Chronic Changes In Serum Il-6 And Tnf-Α Following 12 Weeks Of Concurrent Resistance And Aerobic Exercise Are Dependent On Exercise Mode And May Affect Adaptation, Brad S. Lambert, Justin P. Dobson, Stephen F. Crouse
Chronic Changes In Serum Il-6 And Tnf-Α Following 12 Weeks Of Concurrent Resistance And Aerobic Exercise Are Dependent On Exercise Mode And May Affect Adaptation, Brad S. Lambert, Justin P. Dobson, Stephen F. Crouse
International Journal of Exercise Science: Conference Proceedings
PURPOSE: To examine physiological responses to concurrent resistance and land treadmill training (RT-LTM) compared to concurrent resistance and aquatic treadmill training (RT-ATM) and the chronic effect of each on serum TNF-α and IL-6 (cytokines associated with chronic inflammation, CVD, and skeletal muscle metabolism). METHODS: Twenty-six untrained subjects (M: n=13, 98.6±17.1kg, 182.2±6.2cm, 34±11yrs, F: n=13, 78.9±14.0kg, 165.1±5.1cm, 38±11yrs) were screened to assess VO2max, body composition (DEXA), and strength (Lifts: leg press, chest press, leg curl, lat pull, leg ext, triceps push-down, biceps curl). Subjects were then randomized into 2 groups: RT-LTM (M=6, F=7), RT-ATM (M=7, F=6). Each performed progressive …
Influence Of Fitness And Adiposity On Melanocortin-1 And Melanocortin-3 Receptors On Monocytes., Matthew Unthank, Jonathan Woodson, Jason Butler, Gregory Bratton, James Barbee, Michael Chumley, Dennis Cheek, Joel Mitchell, Melody Phillips
Influence Of Fitness And Adiposity On Melanocortin-1 And Melanocortin-3 Receptors On Monocytes., Matthew Unthank, Jonathan Woodson, Jason Butler, Gregory Bratton, James Barbee, Michael Chumley, Dennis Cheek, Joel Mitchell, Melody Phillips
International Journal of Exercise Science: Conference Proceedings
Purpose: While it is known that exercise improves health by reducing systemic inflammation, potential mechanisms remain to be elucidated. The purpose of this study is to examine the influence of fitness and adiposity on the anti-inflammatory melanocortin system as a potential mechanism by which exercise reduces inflammation. Methods: Forty-one men and women (35-55yr), who were free from cardiovascular disease, inflammatory disorders, and not taking medications that affect inflammation, were recruited. Participants were questioned about exercise habits and medical history; then completed testing for body composition and aerobic fitness (VO2max). Subjects were classified as lean/fit (LF, n=14), overweight/fit (OF, …
Exercise-Induced Lymphocyte Apoptosis And Relationship With Level Of Lactate In Smokers And Non-Smokers, Kyung-Shin Park, Yang Lee
Exercise-Induced Lymphocyte Apoptosis And Relationship With Level Of Lactate In Smokers And Non-Smokers, Kyung-Shin Park, Yang Lee
International Journal of Exercise Science
Purposes of this study were 1) to examine the exercise intensity where lymphocyte apoptosis index (AI) is significantly increased in smokers and non-smokers, 2) to find out whether AI is associated with level of lactate (L). METHODS: Fourteen healthy untrained smokers (≥ 1 pack year, n=7) and non-smokers (n=7) aged 18 to 26 were recruited. Each subject conducted three treadmill runs at different intensities randomly. Running distance for all three runs was equivalent to 30 minute run at 70% VO2max. AI and L were analyzed at rest (Pre), immediately after (Post), and 1 h following (1 h post) each run. …
Voluntary Wheel Running During Weight Loss Leads To Differential Changes In Monocytes, Compared To Forced Treadmill Running, Katie C. Carpenter, Kelley Strohacker, Whitney L. Breslin, Thomas W. Lowder, Brian K. Mcfarlin
Voluntary Wheel Running During Weight Loss Leads To Differential Changes In Monocytes, Compared To Forced Treadmill Running, Katie C. Carpenter, Kelley Strohacker, Whitney L. Breslin, Thomas W. Lowder, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
High-fat feeding and subsequent weight gain may contribute to innate immune dysfunction. Weight loss via calorie restriction and exercise represent one means to restore normal immune function. The purpose of the study was to examine how 8- weeks of aerobic exercise and low-fat diet affects weight gain, monocyte concentration, and monocyte cell-surface expression of TLR2, TLR4, CD80, and CD86. For 12- months, 24 male CD-1 mice underwent a pre-treatment phase, consuming either a low fat (10% fat) or high-fat (60% fat) diet ad libitum. Mice were randomly assigned to one of four groups (N=6/group): CN (low-fat sedentary), V-EX (voluntary wheel …
Exercise Prior To Pregnancy Enhances The Suppressive Function Of Tregs In Offspring In A Mouse Model Of Asthma, Hawley E. Kunz, Lydia Montes, Thomas W. Lowder
Exercise Prior To Pregnancy Enhances The Suppressive Function Of Tregs In Offspring In A Mouse Model Of Asthma, Hawley E. Kunz, Lydia Montes, Thomas W. Lowder
International Journal of Exercise Science: Conference Proceedings
Regulatory T cells (Tregs) are a specialized subset of T cells responsible for peripheral tolerance and the mediation of inappropriate immune responses, such as those seen in asthma and allergy. Our lab is interested in the role that exercise plays in Treg responses. Using a mouse model of asthma, we have shown that exercise increases both the number and suppressive function of Tregs. It has been shown that exposing nursing dams to allergens confers protection against airway hyperresponsiveness and inflammation (trademarks of asthma) in pups exposed to the same allergen. In the present study, we wanted to determine if the …
Childhood Overweight Is Associated With Increased Monocyte Concentration And Altered Subset Distribution, Whitney L. Breslin, Craig A. Johnston, Kelley Strohacker, Katie C. Carpenter, Tiffany Davidson, Jennette Palic, Brian K. Mcfarlin, John P. Foreyt
Childhood Overweight Is Associated With Increased Monocyte Concentration And Altered Subset Distribution, Whitney L. Breslin, Craig A. Johnston, Kelley Strohacker, Katie C. Carpenter, Tiffany Davidson, Jennette Palic, Brian K. Mcfarlin, John P. Foreyt
International Journal of Exercise Science: Conference Proceedings
Childhood obesity rates have nearly tripled in the last 30 years. Obesity increases risk for chronic disease. While monocytes, cells of the innate immune system that are altered with obesity, are purported to play an integral role in the development of these chronic diseases, no research has focused on early phenotypic changes in monocytes of overweight children. Two monocytes subsets exist, classic and pro-inflammatory; alterations in number and distribution may be implicated in disease development in obesity. The purpose of this study was to examine the concentration and relative distribution of monocytes among “normal weight” (N=66) and “at risk for …
Effect Of Hsv-1 Infection On The Exercise-Induced Mobilization Of T-Cell Subsets, Emily C.P. Lavoy, J. Austin Bigley, Mark R. Morrison, Guillaume Spielmann, Jerrald L. Rector, Richard J. Simpson
Effect Of Hsv-1 Infection On The Exercise-Induced Mobilization Of T-Cell Subsets, Emily C.P. Lavoy, J. Austin Bigley, Mark R. Morrison, Guillaume Spielmann, Jerrald L. Rector, Richard J. Simpson
International Journal of Exercise Science: Conference Proceedings
Acute exercise mobilizes highly-differentiated memory and senescent T-cells into the blood compartment, which could have important implications for post-exercise immune surveillance. This response differs in individuals with latent cytomegalovirus (CMV) infection (a herpesvirus), but it is unknown if other latent herpesviruses, such as herpes simplex virus type 1 (HSV-1), also influence this exercise-induced immune response. As HSV-1 infects 50% of the US population, this could have implications for many athletes. PURPOSE: To examine the effects of an acute bout of exercise on the frequency and relative proportion of naïve, memory, and senescent T-cells in the peripheral blood in HSV-1 infected …
The Effects Of Increasing Fat Intake On Body Composition And Blood Monocytes In Cd-1 Mice, Kelley Strohacker, Whitney L. Breslin, Katie C. Carpenter, Nadia Agha, Thomas W. Lowder, Brian K. Mcfarlin
The Effects Of Increasing Fat Intake On Body Composition And Blood Monocytes In Cd-1 Mice, Kelley Strohacker, Whitney L. Breslin, Katie C. Carpenter, Nadia Agha, Thomas W. Lowder, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
Purpose Obesity is a chronic, inflammatory state. Blood monocyte concentrations/proportions have been previously used to assess immune inflammatory status in humans. Although mouse monocytes exist in three subsets, shifts among these cells types have not been documented during a period of diet-induced weight gain. Thus, our purpose was to determine longitudinal changes in mouse monocyte subsets during 12 weeks of low, moderate or high-fat feeding. Methods: 40 CD-1 mice were randomly divided into 4 groups (N=10 per group): stock control (13.5% fat, CN), 30% fat (F30), 45% fat (F45) and 60% fat (F60). Non-lethal, saphenous vein blood samples (~70 μL) …
Mixed High-Fat And Stock Diet Caused A Greater Increase In Body Mass Than High-Fat Diet Alone, Katie C. Carpenter, Tiffany Davidson, Brian K. Mcfarlin
Mixed High-Fat And Stock Diet Caused A Greater Increase In Body Mass Than High-Fat Diet Alone, Katie C. Carpenter, Tiffany Davidson, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
Increased dietary palatability may lead to excess food consumption which in turn causes an excess of caloric intake and weight gain. Although most obesity studies in rodents utilize a high-fat diet, food variation, a combination of different diets for example, may actually increase body weight and fat mass beyond that of solely high-fat feeding. Purpose: To examine the changes in weight gain and body composition in mice that consume either a high-fat diet or a combination of high-fat and stock diet. Methods: 10 CD-1 male mice were randomly assigned to one of two groups based on dietary composition (N=5/group). HF …
Delayed Wound Healing: Can Exercise Accelerate It?, K Todd Keylock, Hilary Young
Delayed Wound Healing: Can Exercise Accelerate It?, K Todd Keylock, Hilary Young
International Journal of Exercise Science
Poorly healing wounds affect millions around the world, yet preventive methods and low-cost, effective treatments are few. Wounds heal quickly through well-coordinated phases in those who are healthy and active but can become chronically nonhealing as a result of disease and inactivity. Recently it has been reported that moderate aerobic exercise accelerated healing rates in the aged. High levels of inflammation are known to delay wound healing, and aging and disease are associated with chronically increased inflammation. Therefore, exercise may help speed healing by reducing inflammation to healthier levels not only in the aged, but also in other populations with …
A Rapid, Flow Cytometry-Based Assay For The Determination Of Natural Killer Cell Activity In Isolated Periphery Blood Mononuclear Cells, Emily C.P. Lavoy, Brian K. Mcfarlin
A Rapid, Flow Cytometry-Based Assay For The Determination Of Natural Killer Cell Activity In Isolated Periphery Blood Mononuclear Cells, Emily C.P. Lavoy, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
Natural killer cell activity (NKCA) is an important assessment of innate immunity in humans. Natural killer (NK) cells are known to be affected by exercise; therefore it is of interest to have an efficient method for measuring NKCA in pre- and post-exercise blood samples. Assays for NKCA include incubating human NK cells (effector cells) with stained target cells. The target cells are typically from the human leukemia cell line K562. The number of K562 target cells killed can be determined using a dead cell dye. Control target cells incubated alone provide a measure of spontaneous cell death. PURPOSE: The purpose …
No Impact Of Cmv Or Ebv Seropositivity On The Frequency Of Highly Differentiated T-Cells In Mexican-American Adolescents, Guillaume Spielmann, Craig A. Johnston, Richard J. Simpson, Brian K. Mcfarlin, John P. Foreyt
No Impact Of Cmv Or Ebv Seropositivity On The Frequency Of Highly Differentiated T-Cells In Mexican-American Adolescents, Guillaume Spielmann, Craig A. Johnston, Richard J. Simpson, Brian K. Mcfarlin, John P. Foreyt
International Journal of Exercise Science: Conference Proceedings
Recurring activations of the prevalent latent herpes viruses Cytomegalovirus (CMV) and Epstein-Barr virus (EBV) induces immune cell division leading to the premature terminal differentiation of T-cells. Terminally differentiated T-cells are known to accumulate with age causing a reduction in the naïve T-cell repertoire, which compromises the ability of the adaptive immune system to respond to novel pathogens. Although CMV and EBV seropositivity are hallmarks of the “immune risk profile” and are known to influence the frequency of terminally differentiated T-cells and increase infection risk in adults, it is not known if CMV or EBV impacts on the frequency of these …
The Effects Of Exercise And A Low-Fat Diet On Monocyte Tlr Expression And Disease Risk In Mice, Katie C. Carpenter, Kelley Strohacker, Whitney Breslin, Nadia Agha, Thomas W. Lowder, Richard J. Simpson, Brian K. Mcfarlin
The Effects Of Exercise And A Low-Fat Diet On Monocyte Tlr Expression And Disease Risk In Mice, Katie C. Carpenter, Kelley Strohacker, Whitney Breslin, Nadia Agha, Thomas W. Lowder, Richard J. Simpson, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
Excess adiposity increases systemic inflammation, which is implicated in various diseases. Physical activity is a common treatment to reverse weight gain and increased disease risk; however, little evidence is available to determine if forced or voluntary exercise is more effective in mice. Such information has implications for the refinement of human exercise interventions. PURPOSE: To determine if 8-weeks of exercise combined with low-fat feeding reverses changes in disease risk, monocyte concentration and monocyte TLR2/TLR4 expression. METHODS: For 12-months, 24 CD-1 mice underwent pre-treatment, consuming either a low-fat (10% kcal from fat, N=6) or high-fat (60% kcal from fat, N=18) diet …
Cell-Surface Receptor Expression On Monocytes Of Young And Old Mice, Kelley Strohacker, Whitney L. Breslin, Brian K. Mcfarlin
Cell-Surface Receptor Expression On Monocytes Of Young And Old Mice, Kelley Strohacker, Whitney L. Breslin, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
Background. Monocyte assessment is used in aging research. In humans, reduced expression of toll-like receptors, T-lymphocyte priming receptors and increases in intracellular adhesion molecules on monocytes have been associated with functional decrements, resulting in increased disease risk. While use of mouse models is extensive in aging research, mouse monocyte assessment is rare. We aimed to evaluate differences in cell-surface protein expression in classic (CD115+/Gr-1high) and non-classic (CD115+/Gr-1low) monocyte subsets of old and young mice. Methods. Venous blood was drawn from 18 old (80-wks) and 18 young CD-1 mice (15-20-wks). Flow cytometry was used to assess subpopulations of CD115+ monocytes for …
Exercise As A Prevention And Countermeasure To Diet-Induced Weight Gain, Whitney L. Breslin, Kelley Strohacker, Nadia Agha, Katie C. Carpenter, Thomas W. Lowder, Brian K. Mcfarlin
Exercise As A Prevention And Countermeasure To Diet-Induced Weight Gain, Whitney L. Breslin, Kelley Strohacker, Nadia Agha, Katie C. Carpenter, Thomas W. Lowder, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
The purpose of this study was to evaluate the effectiveness of treadmill running at attenuating weight gain and immune dysfunction prior to or during a period of high-fat feeding in outbred CD-1 male mice. Mice were divided into four groups (N=10 mice/group): 4-weeks of treadmill running followed by 4-weeks sedentary (EX-SED), 4-weeks sedentary followed by 4 weeks of treadmill running (SEDEX), 8 weeks of treadmill running (EX), and 8 weeks sedentary (SED). After the first four weeks of the study, all groups began consumption of a high-fat diet to elicit a weight gain response. In order to track immune dysfunction, …
The Impact Of Latent Herpesvirus Infections On The Mobilization Of Recent Thymic Emigrants And Extrathymic T-Cells In Response To Acute Aerobic Exercise In Man, Jerrald L. Rector, Guillaume Spielmann, Austin J. Bigley, Richard J. Simpson
The Impact Of Latent Herpesvirus Infections On The Mobilization Of Recent Thymic Emigrants And Extrathymic T-Cells In Response To Acute Aerobic Exercise In Man, Jerrald L. Rector, Guillaume Spielmann, Austin J. Bigley, Richard J. Simpson
International Journal of Exercise Science: Conference Proceedings
T-cells typically mature in the thymus gland, which eventually succumbs to age-related atrophy, resulting in a decreased naïve T-cell repertoire in middle to later years. Aged individuals and those with persistently reactivating herpesvirus infections have an increased reliance on the extrathymic maturation of T-cells due to the shrinking effects that age and latent viral infection has on the naïve T-cell repertoire. Acute bouts of aerobic exercise are known to mobilize T-cells that exhibit both a naïve and late-stage differentiation phenotype into the blood compartment; however, it is not known if recent thymic emigrants (RTE) or extrathymic T-cells contribute to the …
The Impact Of Latent Cmv Infection On Nk-Cell Mobilization And Expression Of Klrg1 And Cd57 In Response To Acute Exercise., Austin B. Bigley, Guillaume Spielmann, Jerrald L. Rector, Richard J. Simpson
The Impact Of Latent Cmv Infection On Nk-Cell Mobilization And Expression Of Klrg1 And Cd57 In Response To Acute Exercise., Austin B. Bigley, Guillaume Spielmann, Jerrald L. Rector, Richard J. Simpson
International Journal of Exercise Science: Conference Proceedings
Natural killer (NK) cells are cytotoxic effectors of the innate immune system that are able to distinguish healthy autologous cells from tumors and virally infected cells. NK-cells kill the targeted cells by releasing cytotoxic proteins, a process that is governed by inhibitory surface receptors, such as KLRG1. Additionally, activated NK-cells are able to proliferate in response to immunological stimuli, a process that is inhibited in NK-cells expressing the senescence marker CD57. Acute bouts of exercise are known to mobilize NK cells into the blood compartment, which could alter immunity; however, whether or not exercise alters NK-cell KLRG1 and CD57 expression …
Serum Il-6 Is Increased During Performance Cycling After Energy Drink Consumption, Jonathan Woodson, Melody Phillips, Jacob Ross, Kelyn Rola, Kenneth Christensen
Serum Il-6 Is Increased During Performance Cycling After Energy Drink Consumption, Jonathan Woodson, Melody Phillips, Jacob Ross, Kelyn Rola, Kenneth Christensen
International Journal of Exercise Science: Conference Proceedings
Energy Drinks (ED) have become popular preexercise supplements due to the stimulant effects of their ingredients such as caffeine. Other ingredients including carbohydrate (CHO), glucuronolactone and taurine may also contribute to performance enhancement. Purpose: The purpose of this study was to investigate the influence of energy drink consumption on cycling performance, substrate oxidation and immune-related variables. Methods: This study was a blinded, randomized, cross-over design with 3 experimental conditions. Eleven trained male cyclists (mean age 33.4±8.9 yr, body mass 81±7.6 kg, VO2max 51.72±3.4 ml*kg-1*min-1) consumed 3 different 500 ml beverages: 1) Energy Drink (ED1- 2.0g taurine, 1.2 g glucoronolactone, 160 …
Consequences Of Weight Cycling: An Increase In Disease Risk?, Kelley Strohacker†, Katie C. Carpenter†, Brian K. Mcfarlin‡
Consequences Of Weight Cycling: An Increase In Disease Risk?, Kelley Strohacker†, Katie C. Carpenter†, Brian K. Mcfarlin‡
International Journal of Exercise Science
Research indicates that weight cycling, or “yo-yo dieting” is a common occurrence in obese populations. The long term negative health consequences of weight cycling are debated and it is unclear whether or not this weight change pattern poses a greater disease risk compared to obesity maintenance. This review discusses the prevalence of weight cycling and physiological alterations occurring during weight loss that promotes weight regain. We also discuss the effect weight regain has upon adipose tissue in terms of rate and type of accumulation. Also within this review are discussions surrounding the previously published literature based upon human and rodent …
Exercise Induced Alterations In Rat Monocyte Number, Morphology, And Function, Marcia Guereschi†, Jonato Prestes‡, Felipe Donatto‡, Rodrigo Dias‡, Anelena Frollini‡, Clílton Ferreira‡, Claudia Cavaglieri‡, Adrianne Palanch‡
Exercise Induced Alterations In Rat Monocyte Number, Morphology, And Function, Marcia Guereschi†, Jonato Prestes‡, Felipe Donatto‡, Rodrigo Dias‡, Anelena Frollini‡, Clílton Ferreira‡, Claudia Cavaglieri‡, Adrianne Palanch‡
International Journal of Exercise Science
The purpose of this study was to verify the histophysiological alterations in monocytes and macrophages induced by short periods of exercise. Male Wistar rats (age = 2 months, body weight = 200g) were divided into seven groups (N=6 each): sedentary control (C), groups exercised (swimming) at low intensity for 5 (5L), 10 (10L), and 15 minutes (15L), and groups exercised at moderate intensity for 5 (5M), 10 (10M) or 15 minutes (15M). At moderate intensity the animals carried a load of 5% of body weight on their backs. Blood monocytes were evaluated for quantity and morphology, and peritoneal macrophages were …