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Colocalization And Distribution Of Hcn4 Channels And A Maguk Protein In Mammalian Sinoatrial Myocytes, Padmastuti Akella
Colocalization And Distribution Of Hcn4 Channels And A Maguk Protein In Mammalian Sinoatrial Myocytes, Padmastuti Akella
Honors Scholar Theses
The sinoatrial node is known as the pacemaker of the heart. It controls the rate at which action potentials are generated and propagate through the sinoatrial myocytes. This thesis is an attempt to identify the colocalization of a specific ion channel (HCN4 channel) with a class of scaffolding protein (MAGUK proteins) within the sinoatrial myocyte. The colocalization of these two variables was performed using immunocytochemistry on murine sinoatrial myocytes and experimental results revealed the existence of a strong colocalization between the two variables. Images collected from the confocal microscope and analyzed using ImageJ software revealed a strong dependent correlation between …