Toxicology Commons^™

Virginia Wild-Caught Blue Catfish: Nutrition And Contaminant Analysis, Robert A. Fisher

Reports

The blue catfish, Ictalurus furcatus, is an introduced freshwater species in Virginia’s tributaries to the Chesapeake Bay. Since its introduction in the 1970’s, significant recreational and commercial fisheries have developed within upriver, largely freshwater environments. Blue catfish are voracious, opportunistic predators, feeding on a variety of natural prey items, including native fish and shellfish species. Their varied diets result in a more nutrient-rich fish compared to farmraised catfish (USDA Food and Nutrient Database https://fdc.nal.usda.gov/fdc-app. html#/?query=catfish) on the market, which are typically fed a plant-based diet.

In recent years, blue catfish populations have expanded from upriver freshwater habitat to major brackish-water …

Dihydrodinophysistoxin-1 Produced By Dinophysis Norvegica In The Gulf Of Maine, Usa And Its Accumulation In Shellfish, Jonathan R. Deeds, Whitney L. Stutts, Mary Dawn Celiz, Jill Macleod, Amy E. Hamilton, Bryant J. Lewis, David W. Miller, Kohl Kanwit, Juliette L. Smith, Et Al

VIMS Articles

Dihydrodinophysistoxin-1 (dihydro-DTX1, (M-H)⁻m/z 819.5), described previously from a marine sponge but never identified as to its biological source or described in shellfish, was detected in multiple species of commercial shellfish collected from the central coast of the Gulf of Maine, USA in 2016 and in 2018 during blooms of the dinoflagellate Dinophysis norvegica. Toxin screening by protein phosphatase inhibition (PPIA) first detected the presence of diarrhetic shellfish poisoning-like bioactivity; however, confirmatory analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS) failed to detect okadaic acid (OA, (M-H)⁻m/z 803.5), dinophysistoxin-1 (DTX1, (M-H)⁻m/z 817.5), or dinophysistoxin-2 (DTX2, (M-H) …

The Effects Of Copper Exposure On Fish Locomotion And Predator-Prey Interactions, Tiffany N. Yanez

FIU Electronic Theses and Dissertations

This study determined the effects of the copper water quality criterion (WQC) by the EPA on [1] swimming performance and [2] predator-prey interactions of the Sailfin Molly, across a salinity gradient. Fish acclimated to FW (0 ppt) and 8-ppt saltwater were exposed to 11.3 and 8.44 µg/L Cu for 96 h, respectively. At the end of the exposures, fish swimming performance was determined by using the critical swimming speed, U_crit, the speed at which a fish cannot longer maintain position in the water column. U_critwas then measured again after a 4-week depuration period to determine if …

Development And Application Of Aquatic Toxicology Studies For The Assessment Of Impacts Due To Chemical Stressors Using Non-Standard Indigenous Organisms, Abraham Jeffrey Smith

FIU Electronic Theses and Dissertations

Research in the multidisciplinary science of ecotoxicology is crucial to assess injuries to ecosystem resources from chemical spills or other stressors used to support environmental decision-making. Established guidelines recommend the use of non-standard native species in toxicity investigations. This work focused on the use of native species for aquatic toxicity assessment to make more relevant conclusions on the potential for adverse biological effects to occur as a result to single chemical exposures or exposures to a complex mixture like oil. We apply these studies to investigate petroleum product impacts from the Deepwater Horizon incident and concerns for metal toxicity in …

Fate Of Methoprene In Temperate Salt Marsh Ditches Following Aerial Applications, David J. Tonjes, Anne E. Mcelroy, Robin K. Barnes, Dominick V. Ninivaggi, Walter Dawydiak, Gregory T. Greene, Bruce J. Brownawell

Technology & Society Faculty Publications

Aerial applications of liquid methoprene are used in salt marshes to control mosquitoes by preventing adult emergence. Despite concern about toxicity to non-target organisms, little is known about environmental concentrations after applications, nor methoprene's persistence in salt marsh environments. Aqueous and sediment samples were collected from two marshes receiving weekly applications. Aqueous samples were collected as early as 30 minutes after applications and as long as nine days afterwards; sediment samples were taken within hours of application and as long as 19 days post-application. Use of time-of-flight liquid chromatography – mass spectral analysis allowed for ultra low detection limits (0.5 …

Remobilization Of Heavy Metals By Mangrove Leaves, Hanan Almahasheer, Oscar Serrano, Carlos M. Duarte, Xabier Irigoien

Research outputs 2014 to 2021

Several studies have been carried out on heavy metal pollution in mangrove ecosystems. However, the role of mangroves in heavy metal remobilization is still relatively unknown. On one side, mangrove woody organs and soils sequester heavy metals for long time periods, but on the other hand, senescence of mangrove leaves may return these metals collected by roots to the upper layers of the soil. Here, we analyzed the concentration of chemical elements (Al, As, Cd, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Sr, V, and Zn) as a function of age in mangrove leaves to understand heavy metals retention by …

Molecular Measurement Of Toxicity In Fish; Case Examples And Policy Implications, Jessica A. Freedman

Senior Honors Projects

Stormwater and oil are common urban contaminants that can be harmful to fish species. One way of recognizing exposed and impaired fish is by monitoring gene expression and gene induction. This study focused on the identification and validation of reference genes for measuring contaminant-induced changes in gene expression due to urban influence. In this study, reference genes (which are genes used to normalize data and remain consistent in varying exposures regardless of organism and tissue type) were established. Six genes were identified as reference genes (ef1a, wdtc1, mtm1, spop, rxrba and tuba1) from a longer list of potential …

Acute Toxicity Assessment Of N,N-Diethyl-M-Toluamide (Deet) On The Photosynthetic Activity Of The Dinoflagellate Gymnodinium Instriatum, Eloy Martinez, Sylvia Velez, Marietta Mayo, Miguel Sastre

Faculty Research & Creative Activity

Despite the ubiquitous occurrence of N, N-diethyl-m-toluamide (DEET) in aquatic systems, assessments evaluating the toxicity of DEET on phytoplankton species are summed to a single study on a unicellular green alga. In particular, the toxicological effects of DEET in dinoflagellates are unknown. In this study, we employed the mixotrophic dinoflagellate Gymnodinium instriatum as a study system to evaluate acute effects of DEET on the oxygen flux of laboratory cultures. This study reports an inhibitory reaction model of DEET described by the equation y = 4.99 x 0.54, where y represents the percent inhibition of oxygen flux and x represents DEET …

Ambient Toxicity Of Water Samples From Four Locations In The Elizabeth River : A Comparison Of Species Sensitivities, Morris H. Roberts, Peter F. De Lisle

Reports

In the present study, the objective was to evaluate four species for relative sensitivity when challenged with ambient water from four locations in the Elizabeth River, ranging from locations considered to be free of toxicity to an area known to have copper concentrations that exceeded water quality standards in past sampling.

Exocrine Pancreatic Neoplasms In The Mummichog (Fundulus Heteroclitus) From A Creosote-Contaminated Site, John W. Fournie, Wolfgang K. Vogelbein

VIMS Articles

A high prevalence of exocrine pancreatic neoplasms occurred in mummichog, Fundulus heteroclitus, from a creosote-contaminated site in the Elizabeth River, Virginia. A total of 20 neoplasms were found in a group of about 1,300 fish obtained at this site over a 2-yr period. Of 240 fish collected during October 1991, 3.3% had pancreatic neoplasms. Adjusted total lesion prevalence for large adult fish (Size Class III: total length = 75–85 mm; Size Class IV: total length > 85 mm) was 6.7%. Pancreatic neoplasms were not observed in 234 fish collected at this site during May 1991, nor were they found in …

Evaluation Of The Toxicity Of Contaminated Sediments In The James River, Virginia, Morris H. Roberts, Charles J. Strobel

Reports

The Virginia State Water Control.Board, in cooperation with the Department of Chemical Oceanography of the Virginia Institute of Marine Science has recently conducted periodic chemical surveys of organic pollutants in the sediments of the upper James River. The August 1983 survey showed elevated concentrations of polycyclic aromatic hydrocarbons (PAH) at Stations ·4 and 7 (Figure 1 and Table 1). Although the reported concentrations were approximately one order· of magnitude lower than those reported in contaminated areas of the Elizabeth River, they were deemed high enough to warrant_ further study based on reported toxicity of Elizabeth River sediments (Hargis et al. …

The Role Of Filter-Feeding Organisms In Concentration Of Suspended Solids Containing Kepone Into Bottom Deposits, Dexter S. Haven, Reinaldo Morales-Alamo

Reports

Filter-feeding marine animals such as molluscs, tunicates and barnacles filter particles as small as 1 micron from suspension during their feeding process and void them as fecal pellets. These settle at much faster rates than their component particles. Feces or pseudofeces which settle are termed biodeposits. Eighty-two to 93 percent by volume of the particles in the biodeposits of several species of molluscs are smaller than 4 microns; the range in size of those particles is about 1 to 10 microns (Haven and Morales-Alamo, 1973). (...)

Uptake Of Kepone By Oysters Exposed To Contaminated Sediments Mixed With Lignite, Dexter S. Haven, Reinaldo Morales-Alamo

Reports

Preliminary studies conducted by personnel of the Allied Chemical Corporation at Morristown, N. J. suggested that coa] would be used to adsorb Kepone from an aquatic substrate. .The possibility of using coal to bind Kepone contaminating the natural environment indicated further preliminary exploration of the matter.

The Virginia Institute of Marine Science undertook a laboratory study with oysters under contract with the Allied Chemical Corp. to explore that possibility. Lignite was mixd on a 1:10 dry-weight ratio with sediments contaminated with Kepone.