Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- 3T3 Cells (1)
- 9-aminoacrodine (1)
- Animals (1)
- Antineoplastic Agents (1)
- Biological (1)
-
- CRISPR-Cas Systems (1)
- Cancer treatment (1)
- Cell Cycle (1)
- Cyclotherapy (1)
- Cytoprotection (1)
- Cytotoxins (1)
- DNA Damage (1)
- Drug Resistance (1)
- Drug Resistance, Neoplasm (1)
- Gene Editing (1)
- Gene Targeting (1)
- Mice (1)
- Models (1)
- Models, Biological (1)
- Neoplasm (1)
- Nuclear Proteins (1)
- Post-Transcriptional (1)
- Protein Biosynthesis (1)
- RBG inhibition (1)
- RNA Processing (1)
- RNA Processing, Post-Transcriptional (1)
- RNA-Binding Proteins (1)
- RRNA maturation (1)
- Ribosome biogenesis (1)
- Ribosomes (1)
Articles 1 - 2 of 2
Full-Text Articles in Toxicology
9-Aminoacridine Inhibits Ribosome Biogenesis And Synergizes With Cytotoxic Drugs To Induce Selective Killing Of P53-Deficient Cells, Leonid Anikin, Dimitri G Pestov
9-Aminoacridine Inhibits Ribosome Biogenesis And Synergizes With Cytotoxic Drugs To Induce Selective Killing Of P53-Deficient Cells, Leonid Anikin, Dimitri G Pestov
Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship
Common cancer treatments target rapidly dividing cells and do not discriminate between cancer and normal host cells. One approach to mitigating negative side‐effects of cancer treatment is to temporarily arrest cell cycle progression and thus protect normal cells during cytotoxic treatments, a concept called cyclotherapy. We recently proposed that transient inhibition of post‐transcriptional steps of ribosome biogenesis (RBG) can be used to selectively arrest p53‐positive host cells and not p53‐null cancer cells. In this study, we investigated whether cytoprotective RBG inhibition can be achieved through small molecule treatment.
Inhibition Of Post-Transcriptional Steps In Ribosome Biogenesis Confers Cytoprotection Against Chemotherapeutic Agents In A P53-Dependent Manner, Russell T Sapio, Anastasiya N Nezdyur, Matthew Krevetski, Leonid Anikin, Vincent J Manna, Natalie Minkovsky, Dimitri G Pestov
Inhibition Of Post-Transcriptional Steps In Ribosome Biogenesis Confers Cytoprotection Against Chemotherapeutic Agents In A P53-Dependent Manner, Russell T Sapio, Anastasiya N Nezdyur, Matthew Krevetski, Leonid Anikin, Vincent J Manna, Natalie Minkovsky, Dimitri G Pestov
Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship
The p53-mediated nucleolar stress response associated with inhibition of ribosomal RNA transcription was previously shown to potentiate killing of tumor cells. Here, we asked whether targeting of ribosome biogenesis can be used as the basis for selective p53-dependent cytoprotection of nonmalignant cells. Temporary functional inactivation of the 60S ribosome assembly factor Bop1 in a 3T3 cell model markedly increased cell recovery after exposure to camptothecin or methotrexate. This was due, at least in part, to reversible pausing of the cell cycle preventing S phase associated DNA damage. Similar cytoprotective effects were observed after transient shRNA-mediated silencing of Rps19, but not …