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Human and Clinical Nutrition Commons

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2015

Cloning

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Full-Text Articles in Human and Clinical Nutrition

Cloning And Expression Of Ebi3 And P28 Subunits Of Human Interleukin 27 In E. Coli, Ameneh Koochaki, Mojgan Bandehpour, Sam Massahi, Mahrou Sadri, Bahram Kazemi Jan 2015

Cloning And Expression Of Ebi3 And P28 Subunits Of Human Interleukin 27 In E. Coli, Ameneh Koochaki, Mojgan Bandehpour, Sam Massahi, Mahrou Sadri, Bahram Kazemi

Department of Nutrition and Health Sciences: Faculty Publications

Background: Interleukin (IL)-27 is a heterodimeric cytokine belonging to IL-12 and IL-23 families, secreted by antigen presenting cells(APCs). The IL-27 is composed of 2 subunits: Epstein-Barr virus induced gene 3 (EBI3) and p28. IL-27 is an anti-inflammatory cytokine which has an inhibitory effect on Th17 population and suppress the IL-17 expression. It is suggested that IL-27 could be a potent drug candidate for treating auto immune diseases.

Materials and Methods: The EBI3 and p28 subunits of human interleukin 27 were constructed into plasmid vectors; they sub-cloned into pETDuet-1 expression vector in restriction sites of BamHI, SacI and NotI. Subsequently the …