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Full-Text Articles in Nutrition
The Effect Of Postruminal Amino Acid Flow On Muscle Cell Proliferation And Protein Turnover, James M. Reecy, James E. Williams, Monty S. Kerley, Ruth S. Macdonald, William H. Thornton, Julie L. Davis
The Effect Of Postruminal Amino Acid Flow On Muscle Cell Proliferation And Protein Turnover, James M. Reecy, James E. Williams, Monty S. Kerley, Ruth S. Macdonald, William H. Thornton, Julie L. Davis
Ruth S. MacDonald
An experiment was conducted to characterize the effects of postruminal administration of casein, glutamine, cornstarch, and water on protein turnover and in vitro muscle cell proliferation. Four MARC III steers (205 kg) were fed a protein-restricted bromegrass hay-based diet (2.86 Mcal of DE/kg and 13.6 g of N/kg). Using a 4 x 5 Latin square arrangement balanced for residual effects, casein and glutamine, equal to 50% of basal dietary nitrogen intake, cornstarch, isocaloric with casein infusion, or an equal volume of water was continuously infused into the abomasum of steers. Blood samples, collected every 2 h for 24 h after …
Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For Quantification Of Skeletal Muscle Calpastatin, M. E. Doumit, Steven M. Lonergan, J. R. Arbona, J. Killefer, M. Koohmaraie
Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For Quantification Of Skeletal Muscle Calpastatin, M. E. Doumit, Steven M. Lonergan, J. R. Arbona, J. Killefer, M. Koohmaraie
Steven M. Lonergan
An indirect antibody ELISA was developed for rapid and sensitive quantification of skeletal muscle calpastatin. Polyclonal antibodies were raised in rabbits against recombinant calpastatin, corresponding to domains 2, 3, and 4 of bovine skeletal muscle calpastatin. Western blot analysis revealed that these antibodies specifically recognize an immunoreactive calpastatin protein of approximately 130 kDa in prerigor skeletal muscle extracts. The intensity of the immunoreactive bands corresponds qualitatively with assayable calpastatin activity. For ELISA development, optimum dilutions of sample, primary anti-calpastatin antibody, and peroxidase-conjugated secondary antibody were determined by titration. A dilution optimum for coating of Immulonâ 4 (Dynatech) plates was observed …