Nutrition Commons^™

The Probiotic Effect Of Clostridium Cochlearium Is Associated With Significant Change In Short-Chain Fatty Acid Metabolism And Gut Microbiota, Qing Ai

Wayne State University Dissertations

The prevalence of obesity is rising steadily across the world, which increases the risk of many metabolic diseases and life-threatening illnesses. A specific strain, Clostridium cochlearium (C. cochlearium), reported as a butyrate producer, could have potential probiotic effects against obesity. The objective of this study was to evaluate the effects of dietary supplementation of C. cochlearium on a high-fat diet-induced obese (DIO) mouse model. The 16S rRNA sequencing of mice gut microbiome was performed at the end of the experimental period to identify the changes in gut microbial composition, investigate possible functional genes, and elucidate potential mechanisms. Thirty-six C57BL/6 6-8 …

Health And Nutrition Status Of Hemodialysis Patients During Ramadan In Saudi Arabia., Bayan Mansour Tashkandi

Wayne State University Dissertations

During Ramadan R month, Muslims abstain from eating, drinking, and medication from sunrise to sunset, and this causes some changes in their lifestyle and often eating habits (quantity and quality). These changes could lead to affect their health and nutritional status. Among Hemodialysis (HD) patients, malnutrition is common, and it is associated with mortality. Some HD patients opt to fast R which could have an impact on their nutritional and health status. Thus, the primary objective of this study was to document nutritional changes before, during, and after Ramadan on biochemical and health parameters in HD patients. A prospective cohort …

Characterization Of Clostridium Cochlearium As A Potential Probiotic For Obesity Management, Paba Edirisuriya

Wayne State University Dissertations

ABSTRACTCHARACTERIZATION OF CLOSTRIDIUM COCHLEARIUM AS A POTENTIAL PROBIOTIC FOR OBESITY MANAGEMENT

ByPABA EDIRISURIYA MAY 2021 Advisor: Dr. Kequan Zhou Major: Nutrition and food science Degree: Doctor of Philosophy Emerging evidence indicates that manipulation of gut microflora is a potential therapeutic approach for managing obesity. Probiotic effects on host weight reduction have repeatedly been revealed through previous studies. Clostridium cochlearium is a butyrate-producing, spore-forming bacteria that have been reported to present in the mammalian gut. Our simulated Invitro digestion model revealed that C. cochlearium could survive in the unfavorable conditions of the human gastrointestinal tract, including low pH (pH2), high bile …

The Relationship Between Excessive Folic Acid Intake And Genome Instability In Human Lymphocytes, Khadijah Ibrahim Alnabbat

Wayne State University Dissertations

Since its synthesis in 1945, Folic acid (FA) is widely used as a supplement/additive to our food due to its stability and high bioavailability. FA is proposed to alleviate anemia and reduce neural tube defects (NTD). As a provitamin, FA is activated through a two-step reaction catalyzed by dihydrofolate reductase (DHFR) in the folate metabolism pathway. FA activation rate is found to be slow in humans. After the implementation of mandatory food fortification program of grain and grain products with folic acid in the USA in 1998, many concerns have been raised about the unintended deleterious consequence of exposing the …

Nutrition And Health Status Of Hemodialysis Patients In Dhaka, Bangladesh, Tanjina Rahman

Wayne State University Dissertations

Methods to identify patients at risk for End stage renal disease (ESRD) are a high priority in Bangladesh, where kidney transplants/dialysis options are limited and costly. Every year, 35,000 to 40,000 people reach ESRD in Bangladesh, but currently available facilities can hardly accommodate only 9000 to 10,000 new patients with twice weekly dialysis and the remaining 66% have no access to any kind of renal replacement therapy (RRT) in the form of dialysis or transplantation. Nutrition is an important factor in maintaining good health of hemodialysis patients. However, data on nutritional status of Bangladeshi dialysis patients is limited and is …

Impact Of Dietary Patterns On Health Outcomes In African American Maintenance Hemodialysis Patients, Dina Angela Tallman

Wayne State University Dissertations

In the United States, African Americans (AA) suffer a disproportionate burden of ESRD, accounting for 35% of all dialysis patients, and are 3.7 times more likely to progress to ESRD than whites. This increase in CKD risk is partially attributed to higher rates of hypertension, diabetes mellitus, and cardiovascular disease. Paradoxically, once on hemodialysis, AA have a survival advantage over whites, likely due to multiple factors including nutritional status, inflammation, psychosocial status, and genetic variation.

Although both the dialysis procedure and pharmacological treatments are effective at reducing the uremic toxin load, HD patients are still encouraged to adhere to strict …

Role Of Dyslipidemia On Lipid Metabolism In Maintenance Hemodialysis Patients, Eno Latifi

Wayne State University Dissertations

Maintenance hemodialysis (MHD) patients experience various abnormalities such as systemic inflammation (SI), oxidative stress (OS), and dyslipidemia (D). Defined as an imbalance of plasma lipids, lipoproteins, and lipid metabolism enzymes, D has been associated with a rise in morbidity and mortality within ESRD patients due to cardiovascular disease (CVD). However, the contribution of each of these parameters to D is poorly understood; moreover, the impact of the following parameters on dyslipidemia in different ethnicities is unknown. Hence, the objective of this study was to characterize D in a multi-ethnic cohort of ESRD patients. We hypothesized that the degree of dyslipidemia …

Anti-Cancer Effects Of Tocotrienols In Nsclc, Lichchavi Raj Raj

Wayne State University Dissertations

Lung cancer is one of the leading causes of death among cancers, with non-small cell lung cancer (NSCLC) accounting for 80-85% of all lung cancers and a five-year survival rate of 5 % at stage IIIB. Delta-tocotrienol (δT) including other tocotrienol isomers has been shown to exhibit anti-tumor activity via inhibition of different signaling pathways in tumors including NSCLC. Previously we reported that δT reduced cell invasion via inhibition of the Notch-1 and NF-κb pathway.

Matrix metallopeptidase 9 (MMP9) dependent cell migration and invasion are key processes in cancer metastasis. Hence, its suppression is a promising strategy for cancer therapeutics. …

The Effect Of Oil Palm Phenolics (Opp) On Pancreatic Ductal Adenocarcinoma (Pdac) In Transgenic Mouse Model, Nurul Huda Razalli

Wayne State University Dissertations

Pancreatic ductal adenocarcinoma (PDAC) is an extremely aggressive form of pancreatic cancer with low survival rates partly due to late diagnosis and poor treatment outcomes. The use of chemotherapy drug, gemcitabine alone often provides minimal benefits. This study explored the in vivo effect of oil palm phenolics (OPP), a water-soluble extract from oil palm in transgenic mouse model of PDAC and its combination with gemcitabine. Administration of 5% dietary OPP was found to be non-toxic in non-PDAC controls. Compared to single agent therapy with either OPP or gemcitabine, OPP-gemcitabine combination showed a superior benefit with profound synergistic effect both as …

Synergistic Effect Of Natural Antimicrobials Produced From Food On Foodborne Pathogens And Effect Of Meat Production Method On Quality And Shelf Life Of Meat, Mahdi Hassan Al-Ali

Wayne State University Dissertations

Meat is highly perishable food since it contains sufficient nutrients needed to support the growth of microorganisms which is effect of nutritional value and spoilage of meat. There are many methods are available to control food pathogens in meat. Using natural antimicrobial may be is a good option because it is provide many benefits. This study showed the synergistic effect of individual and combinations of lactoferrin, lysozyme and nisin in-vitro and in-vivo (in meat) had significant effect (P < 0.05) on reducing the number of foodborne pathogens in meat. Toxigenic Staphylococcus aureus contamination in meat is a major problem in public health due to the production of heat-stable enterotoxins. Also, S.aureus expresses an many array of virulence factors contribute to the ability of S. aureus to cause infection, our results showed that high percentage (42.9) of S. aureus in meat and 80.9 % of S. aureus isolates contained at least one enterotoxin gene. The three most common enterotoxin genes in meat were sea gene (61.9%) followed by seh gene (33.3%) and sei gene (30.1). Some of S. aureus isolates contained more than one enterotoxin gene. In addition, our results indicated that S. aureus isolates from meat showed different antimicrobial resistance levels against fourteen antibiotics. Also, S. aureus multidrug resistant were high in meat. There are different types of meat based on the meat production method. Halal meat is produced without stunning prior to slaughter, resulting in a rapid and complete bleeding of animals. Our results showed that halal meat production method resulted in significantly lower residual heme iron than conventional method with stunning (non-halal method). Microbiological analyses of fresh meat samples showed that halal meat production method had significantly lower (P < 0.05) microbial count than non-halal meat method. Storage of meat is critical because during storage even with proper refrigeration, the meat ultimately undergoes deteriorative changes including microbial spoilage, oxidative changes. Our results indicated that meat production method significantly affects the meat quality not only for fresh meat but also during storage. Microbiological analyses of meat samples during storage for three weeks at 4°C showed that halal meat method had significantly lower (P < 0.05) microbial count than non-halal meat. Physiochemical analyses showed that quality indicators for meat such as lipid oxidation, drip loss, pH, and heme iron content were significantly lower (P < 0.05) in halal meat than non-halal meat during storage. Halal meat processing may be advantageous in reducing bacterial colonization, increasing the usable shelf life, and improving the quality of the fresh and stored meat.

Development Of An Arab Food Frequency Questionnaire And Examination Of Changes In Dietary Patterns In Arab Immigrant Women To The United States, Samiah Alqahtani

Wayne State University Dissertations

INTRODUCTION: Immigrants to the U.S. face challenges and changes regarding to their food intake patterns that may be associated with their health status. However, less is known about the intake patterns among Arab immigrants.

OBJECTIVES 1: To establish a food intake database using 24-hr dietary records from Arab females who immigrated to the U.S. for less than 5 years, 5 to 10 years and greater than 10 years, as well as women still living in SA; 2: To establish an Arab Food Frequency Questionnaire (AFFQ) for future nutrition-related studies with Arab populations.

AIMS 1: To test the hypothesis that as …

Effects Of Dietary Intake Of Antioxidants And Omega-3 Fatty Acids On Free Radical Production In Children At Various Stages Of Chronic Kidney Disease, Linda Marie Sawyer

Wayne State University Dissertations

The purpose of this study was to understand the relationship between dietary intake of antioxidants and omega-3 fatty acids on free radical injury and free radical scavenging in children with chronic kidney disease (CKD) so that complications of kidney failure, especially cardiovascular disease, may be avoided or delayed. Children with CKD on dialysis and post-renal transplantation are at an extremely high risk for cardiovascular morbidity and mortality. We hypothesized that children with CKD who have higher dietary intake of antioxidants and omega-3 FAs, would have lower free radical injury, as measured by 8-Isoprostanes (8-ISOP) and Thiobarbituric Acid Reactive Substances (TBARS) …

The In Vivo Effect Of Oil Palm Phenolics (Opp) In Atherogenic Diet Induced Rats Model Of Alzheimer’S Disease (Ad), Yan Wu

Wayne State University Dissertations

Alzheimer’s disease (AD) is the most common cause of dementia in the aging population. It is characterized by cognitive decline and deposition of ß-amyloid plaques in the hippocampus. It has been shown that hypercholesterolemia induced by high cholesterol diet is associated with AD development. Increased level of oxidative stress has also been observed in AD patients. An important strategy to treat or delay the impairment is based on dietary modification, using food supplements. OPP, a water soluble fraction from oil palm fruit, rich in phenolics has been found to possess significant antioxidant activities. Its beneficial effects on cardiovascular diseases, diabetes …

The Impact Of Folate Restriction On Cancer And Aging: A Mechanistic Analysis, Safa Beydoun

Wayne State University Dissertations

Aging is a multifactorial process associated with alterations in several physiological functions. It increases susceptibility to disease and ultimately results in mortality. Since the associated diseases of aging are highly prevalent in the geriatric population, targeting the aging process as a whole may provide a better way to delay these age related diseases, in turn delaying aging. Various interventions have been shown to delay aging and age related diseases. They impact a major nutrient sensing pathway, mTOR. mTOR signaling is altered in many cancers and its downregulation was shown to delay aging. Other interventions extend lifespan by altering the one …

Isolation And Characterization Of Natural Alpha-Glucosidase Inhibitors From Antioxidant Rich Red Wine Grapes (Vitis Vinifera), Hoda Chaouki Kadouh

Wayne State University Dissertations

Background: Diabetes is currently a global public health problem affecting people at all ages. Dietary antioxidants have been associated with a reduced risk of type 2 diabetes. Grape pomace contains considerable amounts of polyphenols and it has been reported to exhibit an inhibitory activity against alpha- glucosidases. Alpha-glucosidases, in turn, play a major role in controlling starch digestion and therefore postprandial blood glucose, a target for diabetes management.

Objective: This study aims to investigate the anti-diabetes potential of a selection of six grape pomaces and prepare and purify active components in the active variety that specifically inhibit intestinal alpha-glucosidases. The …

Uracil Accumulation Response To Folate Depletion Is A Function Of Dna Repair Imbalance That May Be A Mechanism Of Aging, Hongzhi Ma

Wayne State University Dissertations

Folate is an essential nutrient for human. They carry and transfer one carbon units. They has important biological functions during many reactions, like DNA synthesis, RNA synthesis, DNA methylation, DNA repair, cell division and growth. Through biochemical techniques to determine the gene expression, enzyme activity, uracil level in DNA, and through microbiological assay to determine the folate level, elucidates mechanism of uracil accumulation response to folate depletion. The findings present folate deficiency status affects the uracil DNA glycosylase activity, and uracil initiated DNA base excising repair. DNA repair ability decide whether there is uracil accumulation under the folate depletion condition …

Metabolic Syndrome In Obese African American Adolescents, Yulyu Yeh

Wayne State University Dissertations

Limited research regarding metabolic syndrome (MetS) has been conducted in obese African American adolescents (OAAA) using the International Diabetes Federation IDF criteria. MetS is a constellation of risk factors that increase the risk for chronic diseases and is defined as presence of any 3 or more of the 5 criteria: fasting glucose 100mg/dl, waist circumference 90th percentile, triglycerides 150 mg/dl, blood pressure (BP) 130/85 mmHg and HDL < 40mg/dl. The aim of this study was to assess the incidence of MetS in OAAA by using the IDF criteria and the relationship with diet, fast food consumption, nutrition knowledge, home food environment (HFE), and serum calcium, leptin, 1,25-dihydroxy vitamin D (1, 25 D3) levels. One hundred and fifty one OAAA (mean age: 14.3 ± 1.4 years, 65.6% girls, mean BMI: 37.4±7.2 kg/m2) in a metropolitan area took part in this study. Anthropometric data and fasting blood samples were collected. Participants completed a set of questionnaires regarding diet, fast food consumption, and nutrition knowledge. HFE was completed by the care givers. The MetS rate for OAAA was 61.6% and boys had higher MetS than girls (73.1%, 55.6% respectively). OAAA without MetS had higher serum calcium and 1,25 D3 level but no significant difference were found in food consumption, nutrition knowledge, and HFE. Total cholesterol /HDL ratio and Insulin resistance increased the odds of MetS by 55% and 10% and 1,25 D3 decrease the odds of MetS by 2%. Even though high fast food consumption, lack of nutrition knowledge and poor food environment were negatively associated with obesity, they didn't contribute to MetS. Increase serum vitamin D and calcium level might be beneficial to reduce MetS in OAAA population.

Multifaceted Nutritional Intervention In Hemodialysis Patients, Zulfitri Azuan Mat Daud

Wayne State University Dissertations

Mortality rate in patients undergoing chronic hemodialysis (HD) in the United States remain unacceptably high despite improvement in dialysis technology. Cardiovascular disease (CVD) account for more than 50% of the premature death in this population. Evidences indicate that the accelerated CVD are attributed to by malnutrition, inflammation, dyslipidemia and oxidative stress. Dietary intervention using nutrients with antioxidant, anti-inflammatory, and potential lipid altering properties to correct the aforementioned problems remain inconclusive. Furthermore, such nutritional intervention trial is often hampered by poor compliance related to medical and socioeconomic barriers. Therefore, a series of randomized, double-blind, placebo-controlled, parallel trials were undertaken to document …

Anti-Cancer Effects Of Garcinol In Pancreatic Cancer Transgenic Mouse Model, Nadia Saadat

Wayne State University Dissertations

Adenocarcinoma of pancreas is recognized for its poor prognosis, as it progresses asymptomatically and is rarely diagnosed at early stage. According to American cancer society pancreatic cancer, it has lowest survival rate in all cancer types, with less than 6% five year survival rate. Surgical resection is only possible for 20% of diagnosed cases and current chemotherapy has only 15% response rate. Even the most favored drug, Gemcitabine, increases survival time only by a few months, depending on the stage at diagnosis. Some bioactive food components or nutraceuticals have shown chemopreventive and chemotherapeutic effects without added side effects. Garcinol is …

The Effect Of Proalgazyme And Its Subfractions On Lipid Metabolism In Diet Induced Hypercholesterolemic Hamsters: Correlation With Plasma Metabolomic Profile, Andreea Geamanu

Wayne State University Dissertations

ABSTRACT

THE EFFECT OF PROALGAZYME AND ITS SUBFRACTIONS ON LIPID METABOLISM IN DIET INDUCED HYPERCHOLESTEROLEMIC HAMSTRES:

CORREALTION WITH PLASMA METABOLOMIC PROFILE

by

ANDREEA GEAMANU

December 2013

Advisor: Dr. Smiti Gupta

Major: Nutrition and Food Science

Degree: Doctor of Philosophy

Background: Plasma HDL cholesterol levels are inversely related to cardiovascular disease, which is the leading cause of death worldwide. This study investigated the preventative effect of an algae infusion, ProAlgaZyme (PAZ) and its subfractions (F1, F2, F3, F4) on plasma HDL in a hamster model. Further, the study aimed to identify the biologically active fraction of PAZ and to determine the …

Desensitization Of Basal Cell Carcinoma To The Anti-Tumoral Effect Of Vitamin D, Rawia Khasawneh

Wayne State University Dissertations

The relationship between Vitamin D and NMSC is not very clear. Most of human studies in literature in regards to vitamin D and NMSC are epidemiological which are inconsistent and inconclusive. Mechanistic studies about BCC are few, and mostly are animal models or cell cultures. The main goal for this study is to interpret the impact of vitamin D status on the onset and progression and possibly treatment of basal cell carcinoma. Three tissue samples were collected from BCC patients (Cancer, Proximal, and Distal), and from cancer-free individuals from southeast Michigan. This study design helps identifying alterations in the genetic …

Intervention Of Non-Small Cell Line Cancer Oncogenic Pathways With Delta-Tocotrienol, Xiangming Ji

Wayne State University Dissertations

Non-small cell lung cancer (NSCLC) is most hostile and leading cause of cancer deaths in the United States. Clinical data has demonstrated that 30 % of NSCLC patients have increased Notch activity and 10% of NSCLC have gain-of-function mutation on Notch-1 gene. Our data demonstrated that delta-tocotrienol could inhibit NSCLC cells proliferation, invasion and induce apoptosis by down-regulation of the Notch-1 signaling pathway. Using microRNA microarray and microRNA transfection, our findings further suggest that delta-tocotrienol is a non-toxic activator of miR-34a which can inhibit NSCLC cell proliferation, induce apoptosis, and inhibit invasion. Last but not the least, We observed that …

The Impact Of Folate Deficiency On The Base Excision Repair Pathway: Analysis Of Enzyme Coordination In Response To Dna Damage And Imbalanced Repair, Amanda Pilling

Wayne State University Dissertations

The condition of folate deficiency has been implicated in carcinogenesis, with the strongest evidence formulated in colon cancer. The role of folate in DNA repair, DNA synthesis and methylation reactions renders this nutrient an valuable target for studying the onset and progression of cancer. Using molecular techniques to determine gene and protein expression, enzyme activity and methylation status elucidates the mechanism of DNA repair and damage in folic acid deficient animals in response to carcinogen. The findings presented in this study indicate failure to remove and repair damage in the condition of folate deficiency and suggest that the accumulation of …

Base Excision Repair, Folate Deficiency And Cancer, Archana Unnikrishnan

Wayne State University Dissertations

Folate, an essential water soluble vitamin has been implicated in the etiology of many types of cancer especially colorectal cancer. Folate deficiency has been reported to incapacitate DNA repair pathways and thereby affect the genomic stability. Our lab has reported previously and here again through this research that folate deficiency affects the DNA damage inducibility of base excision repair pathway. Our study shows the differential effect folate deficiency has on the expression of the genes involved in this pathway. Further, our study shows the increase in preneoplastic lesions in the colon of mice exposed to folate deficiency in response to …

Effects Of Dietary Fat Saturation On Lipoprotein Metabolism In Rodents And Humans, Deepinder Kaur

Wayne State University Dissertations

Consumption of trans-fatty acids (tFA) and saturated fatty acids (SFA) have been associated with higher incidence of coronary heart disease. Experimental and observational data suggests that consumption of PHVO containing tFA, like SFA leads to increased total cholesterol and LDL-cholesterol (LDL-C) but compared to tFA, SFA raises HDL-cholesterol (HDL-C). An attempt to eliminate PHVO from the food supply has resulted in the need to find alternatives which despite their higher dietary fat saturation as compared to naturally occurring vegetable oils, may be preferable to PHVO. However, amongst SFA, the effects on plasma lipoproteins are variable. Thus, we hypothesized that specific …

Metabolomic Profiling To Investigate Effect Of Curcumin In Lung Cancer, Arvind Goja

Wayne State University Dissertations

Lung cancer is the leading cause of cancer deaths in the US, in part due to poor diagnosis. Prognosis can be improved, if detected at an early stage. The objective of this study was to use the metabolomic approach to investigate early potential biomarkers for lung cancer. Golden Syrian hamsters were randomized into two groups (treatment and control). Lung cancer was induced in treatment group by a chemical carcinogen, N-nitrosobis-2-(oxopropyl) amine (BOP) while the control animals were injected with saline. Urine was collected weekly throughout the study and frozen at -800 C. Proton nuclear magnetic resonance (1H NMR) spectra, acquired …

Destruction Of Biological Tetrapyrrole Macrocycles By Hypochlorous Acid And Its Scavenging By Lycopene, Dhiman Maitra

Wayne State University Dissertations

Hypochlorous acid (HOCl) is a potent oxidant generated by the hemoprotein myeloperoxidase. Although HOCl plays an important role in the innate immune response,sustained high levels of HOCl has been implicated to play a harmful role. In several pathological conditions such as atherosclerosis, endometriosis and sickle cell disease where HOCl is elevated there are reports of significant free iron accumulation. Free iron is toxic since it can lead to the generation of other secondary free radicals such as hydroxyl radical by Fenton reaction. The exact source and mechanism by which the free iron is generated is not clearly understood. This work …

Development Of Interventions Aimed At Reducing Obesity And Cardiovascular Disease Risk In A Diverse Population Of College-Age Young Adults, Janice M.W. Rueda

Wayne State University Dissertations

ABSTRACT

DEVELOPMENT OF INTERVENTIONS AIMED AT REDUCING OBESITY AND CARDIOVASCULAR DISEASE RISK IN A DIVERSE POPULATION OF COLLEGE-AGE YOUNG ADULTS

by

JANICE MARIE WHINTER RUEDA

May 2011

Advisor: Dr. Pramod Khosla

Major: Nutrition & Food Science

Degree: Doctor of Philosophy

Obesity in the United States has increased dramatically over the past 30 years, and the country has fallen far short of the stated objective of Healthy People 2010 of reducing the prevalence of adult obesity to 15%. Among children, obesity has increased most significantly in the 12-19 year old age group. It is well established that obesity is associated with …

Synergistic Effects Of Garcinol And Gemcitabine In Enhancing Therapeutic Efficacy In Pancreatic Adenocarcinoma Cells And Its Effect On Microrna Profile, Mansi Anand Parasramka

Wayne State University Dissertations

SYNERGISTIC EFFECTS OF GARCINOL AND GEMCITABINE IN ENHANCING THERAPEUTIC EFFICACY IN PANCREATIC ADENOCARCINOMA CELLS AND ITS EFFECT ON MICRORNA PROFILE

By

MANSI PARASRAMKA

ADVISOR: DR. SMITI V. GUPTA

MAJOR: NUTRITION AND FOOD SCIENCE

DEGREE: DOCTOR OF PHILOSPHY

Human Pancreatic Cancer (PaCa) is one of the most hostile and fourth leading cause of cancer deaths in the United States. Current standard chemotherapeutic agent for advanced PaCa is gemcitabine, a cytotoxic nucleoside analogue which results in modest response due to high degree of inherent and acquired chemo resistance. Forthcoming evidence strongly supports that non-nutritive food components have therapeutic benefits attributable to pleiotropic …

Effect Of Dietary Folate Restriction On Colon Carcinogenesis In Dna Polymerase Β Haploinsufficient Mice, Lisa F. Ventrella Lucente

Wayne State University Dissertations

The data presented in this research is central to establishing the role that the base excision repair pathway (BER) plays in the development and progression of colon cancer when dietary folate is deficient. Both cellular folate restriction and BER deficiencies have been shown to result in the accumulation of endogenous damage and lesions that could eventually develop into carcinogenesis. In this study, a dietary folate deficiency (FD) resulted in a significant increase in aberrant crypt foci (ACF) formation and triggered liver tumorogenesis in wildtype (WT) animals, as did a BER deficiency in DNA polymerase Β haploinsufficient (Β-pol+/-) mice exposed to …