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Dengue Viral Rna Levels In Peripheral Blood Mononuclear Cells Are Associated With Disease Severity And Preexisting Dengue Immune Status, Anon Srikiatkhachorn, Sineewanlaya Wichit, Robert V. Gibbons, Sharone Green, Daniel H. Libraty, Timothy P. Endy, Francis A. Ennis, Siripen Kalayanarooj, Alan L. Rothman
Dengue Viral Rna Levels In Peripheral Blood Mononuclear Cells Are Associated With Disease Severity And Preexisting Dengue Immune Status, Anon Srikiatkhachorn, Sineewanlaya Wichit, Robert V. Gibbons, Sharone Green, Daniel H. Libraty, Timothy P. Endy, Francis A. Ennis, Siripen Kalayanarooj, Alan L. Rothman
Alan Rothman
BACKGROUND: Infection with dengue viruses (DENV) causes a wide range of manifestations from asymptomatic infection to a febrile illness called dengue fever (DF), to dengue hemorrhagic fever (DHF). The in vivo targets of DENV and the relation between the viral burden in these cells and disease severity are not known.
METHOD: The levels of positive and negative strand viral RNA in peripheral blood monocytes, T/NK cells, and B cells and in plasma of DF and DHF cases were measured by quantitative RT-PCR.
RESULTS: Positive strand viral RNA was detected in monocytes, T/NK cells and B cells with the highest amounts …
Dengue Viral Rna Levels In Peripheral Blood Mononuclear Cells Are Associated With Disease Severity And Preexisting Dengue Immune Status, Anon Srikiatkhachorn, Sineewanlaya Wichit, Robert V. Gibbons, Sharone Green, Daniel H. Libraty, Timothy P. Endy, Francis A. Ennis, Siripen Kalayanarooj, Alan L. Rothman
Dengue Viral Rna Levels In Peripheral Blood Mononuclear Cells Are Associated With Disease Severity And Preexisting Dengue Immune Status, Anon Srikiatkhachorn, Sineewanlaya Wichit, Robert V. Gibbons, Sharone Green, Daniel H. Libraty, Timothy P. Endy, Francis A. Ennis, Siripen Kalayanarooj, Alan L. Rothman
Sharone Green
BACKGROUND: Infection with dengue viruses (DENV) causes a wide range of manifestations from asymptomatic infection to a febrile illness called dengue fever (DF), to dengue hemorrhagic fever (DHF). The in vivo targets of DENV and the relation between the viral burden in these cells and disease severity are not known.
METHOD: The levels of positive and negative strand viral RNA in peripheral blood monocytes, T/NK cells, and B cells and in plasma of DF and DHF cases were measured by quantitative RT-PCR.
RESULTS: Positive strand viral RNA was detected in monocytes, T/NK cells and B cells with the highest amounts …