Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Virology
Identification Of A Protein Linked To Nascent Poliovirus Rna And To The Polyuridylic Acid Of Negative-Strand Rna, Ralf F. Pettersson, Victor R. Ambros, David Baltimore
Identification Of A Protein Linked To Nascent Poliovirus Rna And To The Polyuridylic Acid Of Negative-Strand Rna, Ralf F. Pettersson, Victor R. Ambros, David Baltimore
Victor R. Ambros
A protein similar to that previously demonstrated on poliovirus RNA and replicative intermediate RNA (VPg) was found on all sizes of nascent viral RNA molecules and on the polyuridylic acid isolated from negative-strand RNA. 32P-labeled nascent chains were released from their template RNA and fractionated by exclusion chromatography on agarose. Fingerprint analysis using two-dimensional polyacrylamide gels of RNase T1 oligonucleotides derived from nascent chains of different lengths showed that a size fractionation of nascent chains was achieved. VPg was recovered from nascent chains varying in length from 7,500 nucleotides (full-sized RNA) to about 500 nucleotides. No other type of 5' …