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Pathogenic Microbiology Commons

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Full-Text Articles in Pathogenic Microbiology

Identification And Characterization Of Functional Regulatory Variants In S. Cerevisiae, Timothy Read Jan 2015

Identification And Characterization Of Functional Regulatory Variants In S. Cerevisiae, Timothy Read

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Most genetic variants associated with disease occur within regulatory regions of the genome, underscoring the need to define the mechanisms that control differences in gene expression regulation between individuals. I discovered a pair of co-regulated, divergently oriented transcripts, AQY2 and ncFRE6, that are expressed in one strain of S.cerevisiae, ∑1278b, but not in another, S288c. By combining classical genetics techniques with high-throughput sequencing, I identified a trans-acting single nucleotide polymorphism within the transcr¬iption factor RIM101 that causes the background-dependent expression of both transcripts. Subsequent RNA-seq experiments revealed that deletion of RIM101 in both backgrounds abrogated the majority of ...


Effects Of Carbon Quantity And Plant Diversity On Pathogen Suppression By Soil-Borne Streptomyces, Nuttapon Pombubpa Jan 2015

Effects Of Carbon Quantity And Plant Diversity On Pathogen Suppression By Soil-Borne Streptomyces, Nuttapon Pombubpa

Undergraduate Honors Theses

Members of the genus Streptomyces are some of the best candidates for biological control of soil-borne pathogenic bacteria. Antibiotic production by Streptomyces is thought to be crucial for this strain’s ability to compete with other closely related soil microorganisms. Furthermore, Streptomyces is able to not only inhibit the growth of its competitors, but also that of many soil-borne plant pathogens. My thesis assessed Streptomyces performance under varying levels of soil resources (carbon componds that serve as energy sources) and in plots with varying degrees of plant diversity that, in turn, affects soil resources. This project aims to deconvolute the ...