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Pathogenic Microbiology Commons

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Full-Text Articles in Pathogenic Microbiology

Evaluating Edna Metabarcoding As A Mic-Roe-Scopic Net To Catch Salmon Pathogens, Noah Burby Apr 2023

Evaluating Edna Metabarcoding As A Mic-Roe-Scopic Net To Catch Salmon Pathogens, Noah Burby

Honors College

Wild Atlantic salmon in the Gulf of Maine (GOM) is a Distinct Population Segment (DPS) that has been listed since 2000 as endangered by the U.S. Fish and Wildlife Service (USFWS) and the National Oceanic and Atmospheric Administration (NOAA). The current challenge is year-over-year decreases in the number of mature salmon returning to the Penobscot River for reproduction. Early detection of pathogen presence could allow for the identification of infection and the application of corrective measures. Environmental DNA (eDNA) is simply DNA that is collected from environmental samples (e.g., water, air, and soils), which consists of whole microorganisms and genetic …


Impact Of Probiotics On Black Soldier Fly Larvae Transmission Of Stenotrophomonas Maltophilia, Emily Marie Mclaughlin Dec 2021

Impact Of Probiotics On Black Soldier Fly Larvae Transmission Of Stenotrophomonas Maltophilia, Emily Marie Mclaughlin

Honors College

Stenotrophomonas maltophilia is an opportunistic pathogen which can cause a variety of infections, especially in immunocompromised individuals. It has recently been found that black soldier flies can act as carriers for S. maltophilia (Callegari et al., 2020). I have investigated how probiotics impact their vector capabilities. Black soldier fly larvae have been fed substrate spiked with S. maltophilia. After several days of feeding, the larvae were investigated by PCR and plating (Sveensson-Stadler et al., 2011). Two groups of larvae were fed with potatoes infected with S. maltophilia. Persistence of S. maltophilia was determined using PCR by taking samples …


Characterization Of The Microbial Associates Of Nematodes Pathogenic To Myrmica Rubra, Jonathan E. Dumont May 2011

Characterization Of The Microbial Associates Of Nematodes Pathogenic To Myrmica Rubra, Jonathan E. Dumont

Honors College

Pristionchus entomophagus is a necromenic nematode commonly associated with dung beetles (Geotrupes spp.) in Europe. Recently, it has been found in Maine emerging from cadavers of M. rubra, an ant native to Europe that is currently established and pestiferous in Maine (Groden and Stack 2011). Laboratory assays inoculating M. rubra with these nematodes caused significant mortality. This study aimed to characterize the bacterial associates of P. entomophagus in order to further understand its pathogenic relationship with M. rubra. Bacterial samples were collected from the cuticle and gut of the nematodes, and the hemolymph of infected hosts. Single spore isolates were …