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Identification Of Genes Conferring Acid Resistance In Vibrio Parahaemolyticus, Erin Gibbons
Identification Of Genes Conferring Acid Resistance In Vibrio Parahaemolyticus, Erin Gibbons
Honors Scholar Theses
Vibrio parahaemolyticus has the capability to be acid resistant due to known and unknown mechanisms. The goal of this project was to determine possible genes involved in conferring acid resistance of this bacterium. The vp1277 gene (designated as HA) and its downstream gene vp1999 were investigated for their role in acid resistance. Another potential gene that could contribute to acid resistance is vp0820 because of its similarity to the ToxR gene in Vibrio cholera. All three of these knockout strains and the wild type were grown in neutral LB and then tested with acidic LB’s of pH 4.5, 5, and …