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Full-Text Articles in Pathogenic Microbiology
Absence Of Soda Increases The Levels Of Oxidation Of Key Metabolic Determinants Of Borrelia Burgdorferi, Maria D. Esteve-Gassent, Trever C. Smith Ii, Christina M. Small, Derek P. Thomas, J Seshu
Absence Of Soda Increases The Levels Of Oxidation Of Key Metabolic Determinants Of Borrelia Burgdorferi, Maria D. Esteve-Gassent, Trever C. Smith Ii, Christina M. Small, Derek P. Thomas, J Seshu
Peer Reviewed Articles
Borrelia burgdorferi, the causative agent of Lyme disease, alters its gene expression in response to environmental signals unique to its tick vector or vertebrate hosts. B. burgdorferi carries one superoxide dismutase gene (sodA) capable of controlling intracellular superoxide levels. Previously, sodA was shown to be essential for infection of B. burgdorferi in the C3H/HeN model of Lyme disease. We employed two-dimensional electrophoresis (2-DE) and immunoblot analysis with antibodies specific to carbonylated proteins to identify targets that were differentially oxidized in the soluble fractions of the sodA mutant compared to its isogenic parental control strain following …