Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Microbiology
Investigating Streptococcus Pneumoniae And Adenovirus Co-Infections Of Lung Epithelial Cells, Mark Nicholas Calabro
Investigating Streptococcus Pneumoniae And Adenovirus Co-Infections Of Lung Epithelial Cells, Mark Nicholas Calabro
Browse all Theses and Dissertations
Co-infection is common among viruses and bacteria in the human respiratory system. Adenovirus (AdV) and Streptococcus pneumoniae are clinically relevant respiratory pathogens that cause morbidity and mortality in a variety of patient populations with the highest morbidity occurring among immunocompromised individuals, but also prevalent in infants and the elderly. Acute respiratory distress syndrome may become severe in healthy individuals when co-infection with S. pneumoniae and AdV occurs due to synergistic effects of the pathogens on the host. I hypothesized that S. pneumoniae infection decreases AdV transduction of airway epithelia. To test this hypothesis, we utilized the polarized immortalized airway epithelial …
Investigating Streptococcus Pneumoniae And Adenovirus Co-Infections Of Lung Epithelial Cells, Mark Nicholas Calabro
Investigating Streptococcus Pneumoniae And Adenovirus Co-Infections Of Lung Epithelial Cells, Mark Nicholas Calabro
Browse all Theses and Dissertations
Co-infection is common among viruses and bacteria in the human respiratory system. Adenovirus (AdV) and Streptococcus pneumoniae are clinically relevant respiratory pathogens that cause morbidity and mortality in a variety of patient populations with the highest morbidity occurring among immunocompromised individuals, but also prevalent in infants and the elderly. Acute respiratory distress syndrome may become severe in healthy individuals when co-infection with S. pneumoniae and AdV occurs due to synergistic effects of the pathogens on the host. I hypothesized that S. pneumoniae infection decreases AdV transduction of airway epithelia. To test this hypothesis, we utilized the polarized immortalized airway epithelial …
Developing A Quantitative Pcr Assay For Detecting Viral Vector Shedding From Animals, Swathee Chinnasamy
Developing A Quantitative Pcr Assay For Detecting Viral Vector Shedding From Animals, Swathee Chinnasamy
Browse all Theses and Dissertations
Replication deficient viruses have been used widely for replacing, repairing, and deleting target genes. These recombinant viruses are tested on research animals or patients in clinical trials. Although viral vectors distribute in the body, they are also disseminated into the environment through secretion and excretion processes. By studying the extent of shedding, a proper risk assessment can be performed and appropriate biocontainment can be achieved. Adenoviral and lentiviral vectors were produced from commercially available kits. The transgene present in both vector systems was the lacZ reporter gene encoding for β-galactosidase. Primers and probes were designed for the encapsidation region of …