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Full-Text Articles in Microbiology
Genetic Characterization Of Toxoplasma Gondii From Pigs From Different Localities In China By Pcr-Rflp, Hai-Hai Jiang, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Chunlei Su, Shun-Zhou Deng, Xing-Quan Zhu
Genetic Characterization Of Toxoplasma Gondii From Pigs From Different Localities In China By Pcr-Rflp, Hai-Hai Jiang, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Chunlei Su, Shun-Zhou Deng, Xing-Quan Zhu
Microbiology Publications and Other Works
Background
Toxoplasma gondii is a widely prevalent protozoan parasite that causes serious toxoplasmosis in humans and animals. The present study aimed to determine the genetic diversity of T. gondii isolates from pigs in Jiangxi, Sichuan, Guangdong Provinces and Chongqing Municipality in China using multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology.
Methods
A total of 38 DNA samples were extracted from hilar lymph nodes of pigs with suspected toxoplasmosis, and were detected for the presence of T. gondii by semi-nested PCR of B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci, namely, …