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Full-Text Articles in Microbiology
Characterization Of Five Brevibacillus Bacteriophages And Their Genomes, Michael Allen Sheflo
Characterization Of Five Brevibacillus Bacteriophages And Their Genomes, Michael Allen Sheflo
Theses and Dissertations
Brevibacillus laterosporus (B. laterosporus) is a pathogen difficult to distinguish from Paenibacillus larvae (P. larvae), and contributes to Colony Collapse Disorder (CCD) of honeybees. To develop a biocontrol agent to limit its presence, bacteriophages were isolated from Utah County soil samples and used to infect B. laterosporus isolated from Utah County honey and larvae samples. Since CCD is prevalent in Utah beehives, bacteriophage that infect and lyse B. laterosporus may be isolated and characterized. Pathogens were isolated from soil samples, and 16S rRNA gene tests initially identified the strains as P. larvae. Bacteriophages were isolated, purified, and amplified sufficiently to …
Advancing Phage Genomics And Honeybee Health Through Discovery And Characterization Of Paenibacillaceae Bacteriophages, Bryan Douglas Merrill
Advancing Phage Genomics And Honeybee Health Through Discovery And Characterization Of Paenibacillaceae Bacteriophages, Bryan Douglas Merrill
Theses and Dissertations
The Paenibacillaceae family of bacteria includes two species known to infect the hives of honeybees, Paenibacillus larvae and Brevibacillus laterosporus. P. larvae, the causative agent of American Foulbrood (AFB) causes a lethal infection of honeybee larvae, while B. laterosporus is a secondary invader following European Foulbrood (EFB) infection. Increasing antibiotic resistance of P. larvae bacteria has prompted a search for alternative treatment methods for this disease. Bacteriophages are the most diverse life forms on earth and can provide important insights about the bacterial hosts they infect. However, few Paenibacillaceae phages have been isolated or characterized. In this study, the first …
P. Larvae Bacteriophages As A Treatment For American Foulbrood In Honeybees, Bryan Merrill, Sandra Burnett
P. Larvae Bacteriophages As A Treatment For American Foulbrood In Honeybees, Bryan Merrill, Sandra Burnett
Journal of Undergraduate Research
The deadly honey bee disease American Foulbrood (AFB) is caused by the bacterium Paenibacillus larvae. The widespread use of antibiotics to prevent or treat AFB has led to many drug-resistant strains of P. larvae. Bacteriophages can target and destroy specific strains of bacteria and can be used to treat bacterial infections. This study accomplished two main goals. We determined the efficacy of previously isolated P. larvae phages as a treatment for AFB by applying a phage spray to infected beehives. We also isolated P. larvae bacteria from the infected hives we treated and identified phages to better counteract …