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Articles 1 - 5 of 5
Full-Text Articles in Microbiology
Genetic Analysis Of Conserved Residues In Phou Of Escherichia Coli, Stewart G. Gardner
Genetic Analysis Of Conserved Residues In Phou Of Escherichia Coli, Stewart G. Gardner
Theses and Dissertations
The Pho regulon is controlled by the PstSCAB transporter, PhoU, and the two-component proteins, PhoB and PhoR. PhoU is a negative regulator of the Pho regulon under phosphate-replete conditions. How PhoU functions is unknown. Many PhoU homologues are found widely throughout prokaryotic domains. There are several conserved amino acid residues in the PhoU protein. It is hypothesized that these residues play an important role in the function of PhoU. To test this hypothesis, several site directed mutations in the phoU gene have been produced with single amino acid changes in conserved residues. After testing these mutants, it was found that …
Homologous Strand Exchange And Dna Helicase Activities In Plant Mitochondria, Daqing Song
Homologous Strand Exchange And Dna Helicase Activities In Plant Mitochondria, Daqing Song
Theses and Dissertations
Homologous recombination is critical for generating genetic variation in living organisms by exchange and rearrangement of DNA. Most of our knowledge about homologous recombination is limited to processes in bacteria or in eukaryotic nuclei. In E. coli, homologous recombination is dependent on the RecA protein. Higher plant chloroplasts have RecA-like strand exchange activity. However, little is known about these mechanisms in higher plant mitochondria. I have detected a RecA-like strand exchange activity in soybean mitochondria. This activity forms joint molecules in the presence of ATP, Mg2+, and homologous DNA substrates. In addition, the E. coli single-stranded DNA binding (SSB) protein …
Investigation Of The Mechanisms Of Ozone-Mediated Viral Inactivation, Seiga Ohmine
Investigation Of The Mechanisms Of Ozone-Mediated Viral Inactivation, Seiga Ohmine
Theses and Dissertations
Previous studies have established that ozone-oxygen mixtures can be used to inactivate a variety of microorganisms including bacteria, fungi and viruses. Ozone is a potent reactive oxygen species (ROS) that rapidly decays into a variety of additional short half-life ROS which have been shown to cause oxidative damage to biological molecules. I hypothesize that controlled ozone exposure and the subsequent generation of additional ROS would reduce viral infectivity by lipid and/or protein peroxidation. A proprietary ozone-oxygen delivery system was used to inactivate a series of enveloped [herpes simplex virus type-1 strain McIntyre (HSV-1), vaccinia strain Elstree (VAC), vesicular stomatitis virus …
Crustacean Phylogenetic Systematics And Opsin Evolution, Megan L. Porter
Crustacean Phylogenetic Systematics And Opsin Evolution, Megan L. Porter
Theses and Dissertations
Composed of a chromophore bound to an integral membrane protein (opsin), visual pigments are phenotypically characterized by the wavelength of maximal absorption (λmax). The underlying molecular mechanism controlling λmax is the interaction between the opsin amino acid sequence and the chromophore. While a plethora of studies have looked at structure/function relationships in vertebrate opsins, fewer studies have investigated similar issues in invertebrates. Furthermore, those few studies undertaken in invertebrate systems suggest different mechanisms of spectral tuning and photoactivation compared to vertebrate systems. This dissertation research is focused on expanding our knowledge of opsin evolution in invertebrate systems, particularly from non-insect …
Assessment Of Cell Death Parameters In Bovine Parvovirus-Infected Ebtr Cells, Lubna Salah Eldin Abdel Latif
Assessment Of Cell Death Parameters In Bovine Parvovirus-Infected Ebtr Cells, Lubna Salah Eldin Abdel Latif
Theses and Dissertations
Bovine parvovirus (BPV) is a helper-independent parvovirus. It has a small icosahedral capsid with a single stranded DNA genome. It is a highly stable virus with a narrow host range. It causes acute gastroenteritis in calves. It is considered to be a cytolytic virus because it kills the host cells. However, the mechanism by which the virus causes cell death is not known. The work described in this thesis assessed different parameters of cell death in BPV infected embryonic bovine tracheal (EBTr) cells. There are several ways for viruses to induce cell death. Viruses can induce apoptosis in the infected …