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Full-Text Articles in Microbiology
Qcm Aptasensor For Rapid And Specific Detection Of Avian Influenza Virus, Luke Brockman
Qcm Aptasensor For Rapid And Specific Detection Of Avian Influenza Virus, Luke Brockman
Graduate Theses and Dissertations
There has been a need for rapid detection of avian influenza virus (AIV) H5N1 due to it being a potential pandemic threat. Most of the current methods, including culture isolation and PCR, are very sensitive and specific but require specialized laboratories and trained personnel in order to complete the tests and are time-consuming. The goal of this study was to design a biosensor that would be able to rapidly detect AIV H5N1 using aptamers as biosensing material and a quartz crystal microbalance (QCM) for transducing method. Specific DNA aptamers against AIV H5N1 were immobilized, through biotin and streptavidin conjugation, onto …
Moraxella (Branhamella) Catarrhalis: A Molecular Epidemiology Study, Lyndell R. Gill
Moraxella (Branhamella) Catarrhalis: A Molecular Epidemiology Study, Lyndell R. Gill
Electronic Theses and Dissertations
Moraxella (Branhamella) catarrhalis is the third-most-frequently isolated microorganism associated with acute exacerbations of chronic bronchitis in patients during their stay at the Mountain Home VA Medical Center (MHVAMC). In order to develop a practical, epidemiologically-meaningful typing method for M. (B.) catarrhalis, we tested two methods based on analysis of chromosomal DNA for typeability, reproducibility, and ability to differentiate between unrelated strains (discriminatory power, D). M. (B.) catarrhalis isolants from MHVAMC from 7/1/87-6/30/88 were grown overnight in broth and embedded in agarose. DNA was isolated by standard methods. The DNA was subjected to: (1) restriction endonuclease digestion (with either Bgl II …