Laboratory and Basic Science Research Commons^™

Distribution And Proportion Of Immunoreactive Neuron Specific Enoclase Containing Cells In The Rat Pineal Gland, Alfonso Miranda C.

Loma Linda University Electronic Theses, Dissertations & Projects

The distribution and proportion of cytoplasm containing immunoreactive neuron specific enolase (NSE) in the rat pineal have been investigated. Step serial sections of the pineal glands of 7 male Sprague-Dawley rats were stained for NSE using an indirect immunoperoxidase method. The volumes of the pineals and of the regions containing reactive cells were derived from the distance between sections and the sum of the corresponding sectional areas of camera lucida drawings. The proportion of the NSE areas actually consisting of NSE reactive cytoplasm was measured using an image array processor.

Cytoplasm reactive for NSE was consistently localized to the posterior …

The Effect Of Orally Administered Butylated Hydroxytoluene On Herpes Simplex Keratitis, Donald Carson

Masters Theses & Specialist Projects

Some studies have reported in vitro inactivation of membrane enveloped viruses by Butylated hydroxytoluene (BHT). This study investigates the effect of orally administered BHT on primary infections of Herpes simplex virus (HSV) using rabbit corneas as the assay system.

Assigned levels of BHT were incorporated into the diet of New Zealand White rabbits with HSV-1 McKrae strain on the corneal surface of the eyes. The degree of infection was measured by a subjective assay. Rabbits receiving BHT in their diet consistently experienced lower levels of infection. The interpretation of this data is that orally administered BHT can be an effective …

Alternatives To Animal Experimentation, Steven M. Niemi

Experimentation Collection

Alternatives to animal experimentation are highly touted today by animal welfare advocates. Their campaign for adoption of alternatives has caused much discussion and debate within and outside of the biomedical community. The purpose of this paper was to examine the controversy and assess the more common alternatives, including the bacterial mutagenicity assay or Ames test, cell culture, and mathematical models for toxicity prediction. Safety testing of chemicals is the most promising of the fields for alternatives where laboratory animals are used, and incorporation of alternatives with live-animal assays is increasing. However, due to limitations of alternatives in use currently, there …

Protection Of Animals And Animal Experimentation: A Survey Of Scientific Experts, Norbert Lagoni, Joachim Fiebelkorn, Hans-Joachim Wormuth

Experimentation Collection

This article summarizes information from a survey of biomedical scientists, specifically pharmacologists and toxicologists, on the use of laboratory animals and the potential for replacing their use with alternative methods for the development and evaluation of pharmaceutical substances. The majority of those surveyed felt that the alternatives could supplement or complement animal tests, but not replace the tests altogether. However, most favored the use of nonsentient material in safety tests.