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Full-Text Articles in Molecular Genetics
Confirmation Of The Function Of Mrub1080 As Γ-Glutamyl Kinase (Prob) In Meiothermus Ruber, Cale J. Mccormick, Dr. Lori Scott
Confirmation Of The Function Of Mrub1080 As Γ-Glutamyl Kinase (Prob) In Meiothermus Ruber, Cale J. Mccormick, Dr. Lori Scott
Celebration of Learning
This project is part of the Meiothermus ruber genome analysis project, which uses wet lab procedures and computational analysis to gather evidence of orthologous genes between Escherichia coli and Meiothermus ruber. In previous work, bioinformatics evidence supported the hypothesis that the gene Mrub1080 was an ortholog of E. coli proB. We investigated the biological function of Meiothermus ruber genes proB and proBA using the complementation assay. However, functional analysis proved inconclusive. For this particular research project, we confirmed that weakly complementing E. coli proB- null strains actually contained the desired M. ruber proB and proBA genes (inserted into a pKt1 …