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Full-Text Articles in Molecular Genetics
Investigating Mirna Functions In Arabidopsis By Short Tandem Target Mimic (Sttm), Yiyou Gu
Investigating Mirna Functions In Arabidopsis By Short Tandem Target Mimic (Sttm), Yiyou Gu
Dissertations, Master's Theses and Master's Reports
MicroRNA (miRNAs) and other endogenous small RNAs play important role in regulating gene expression in eukaryotes. Till now, several techniques have been developed for miRNA function analysis. Because of the characteristic of miRNAs, the most applicable method for miRNA function research is blocking the whole miRNA family specifically. Here, we report an artificial non-coding transcript termed Short Tandem Target Mimic (STTM) for blocking miRNA activity in Arabidopsis thaliana. The STTM composed of two miRNA target sites with three nucleotides bulge in the miRNA cleavage site, the two-miRNA targets separated by spacer linker. We used this technique blocking several important endogenous …