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Full-Text Articles in Molecular Genetics
Phylogenetic Relationships Of African Microhylid Frogs Inferred From Dna Sequences Of Mitochondrial 12s And 16s Rrna Genes, Simon P. Loader, David J. Gower, Kim M. Howell, Nike Doggart, Mark-Oliver Rödel, Barry T. Clarke, Rafael O. De Sá, Bernard L. Cohen, Mark Wilkinson
Phylogenetic Relationships Of African Microhylid Frogs Inferred From Dna Sequences Of Mitochondrial 12s And 16s Rrna Genes, Simon P. Loader, David J. Gower, Kim M. Howell, Nike Doggart, Mark-Oliver Rödel, Barry T. Clarke, Rafael O. De Sá, Bernard L. Cohen, Mark Wilkinson
Biology Faculty Publications
The phylogenetic relationships of microhylid frogs are poorly understood. The first molecular phylogeny for continental African microhylids is presented, including representatives of all subfamilies, six of the eight genera, and the enigmatic hemisotid Hemisus. Mitochondrial 12S and 16S rRNA sequence data were analysed using parsimony, likelihood and Bayesian methods. Analyses of the data are consistent with the monophyly of all sampled subfamilies and genera. Hemisus does not nest within either brevicipitines or non-brevicipitines. It is possibly the sister group to brevicipitines, in which case brevicipitines might not be microhylids. Phrynomantis and Hoplophryne potentially group with non-African, non-brevicipitine microhylids, in …
Phylogenetic Signal And The Utility Of 12s And 16s Mtdna In Frog Phylogeny, S. Hertwig, Rafael O. De Sá, A. Haas
Phylogenetic Signal And The Utility Of 12s And 16s Mtdna In Frog Phylogeny, S. Hertwig, Rafael O. De Sá, A. Haas
Biology Faculty Publications
Genes selected for a phylogenetic study need to contain conserved information that reflects the phylogenetic history at the specific taxonomic level of interest. Mitochondrial ribosomal genes have been used for a wide range of phylogenetic questions in general and in anuran systematics in particular. We checked the plausibility of phylogenetic reconstructions in anurans that were built from commonly used 12S and 16S rRNA gene sequences. For up to 27 species arranged in taxon sets of graded inclusiveness, we inferred phylogenetic hypotheses based on different apriori decisions, i.e. choice of alignment method and alignment parameters, including/excluding variable sites, choice of reconstruction …
Phylogenetic Analysis Of Segment 10 From African Horsesickness Virus And Cognate Genes From Other Orbiviruses, Rafael O. De Sá, Marla Zellner, Marvin J. Grubman
Phylogenetic Analysis Of Segment 10 From African Horsesickness Virus And Cognate Genes From Other Orbiviruses, Rafael O. De Sá, Marla Zellner, Marvin J. Grubman
Biology Faculty Publications
Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1,4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences were analyzed to assess evolutionary relationships among serotypes using cladistics. Based on this analysis the data support a close relationship between serotypes 4 and 9 and between serotypes 1 and 8 and a closer relationship of serotype 3 to the 4 and 9 group.