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Differential Expression Of Genes During Diapause In The Flesh Fly, Sarcophaga Crassipalpis., Puja Karki
Differential Expression Of Genes During Diapause In The Flesh Fly, Sarcophaga Crassipalpis., Puja Karki
Electronic Theses and Dissertations
The objective of this study was to identify genes that are differentially regulated during diapause when compared with nondiapausing pupae in Sarcophaga crassipalpis. The results of a Suppression Subtractive Hybridization procedure was used to indentify genes that are differentially regulated in both diapause and nondiapausing states while suppressing genes that are common to both states. Randomly picked colonies from both subtractive libraries were isolated and the inserts sequenced. The sequences were analyzed using the bioinformatics tools NCBI, BlastX, Clustal W, etc. Out of 384 clones, 59 genes were found to be upregulated during diapause and 37 genes were found …