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- 11-ketotestosterone (1)
- Algae (1)
- Androgen (1)
- Electroporation (1)
- Gene expression (1)
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- Genome annotation (1)
- Genomic variation (1)
- Immune system (1)
- Immunocompetence handicap hypothesis (ICHH) (1)
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- Nuclear genome engineering (1)
- Parental care (1)
- Phaeodactylum tricornutum (1)
- Prolactin (1)
- Receptor for Hyaluronan-Mediated Motility (1)
- Selectable markers (1)
- Selection (1)
- Single nucleotide polymorphisms (1)
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Articles 1 - 3 of 3
Full-Text Articles in Genomics
Advances In Phaeodactylum Tricornutum Nuclear Engineering, Mark Pampuch
Advances In Phaeodactylum Tricornutum Nuclear Engineering, Mark Pampuch
Electronic Thesis and Dissertation Repository
The marine diatom Phaeodactylum tricornutum has the potential to become an excellent platform for the sustainable production of valuable compounds and pharmaceuticals, but currently large-scale engineering of this organism remains a challenge due factors like inefficient genetic transformation protocols and a lack of accurate genomic data. This thesis addresses these two bottlenecks by (i) optimizing an electroporation protocol to P. tricornutum and (ii) remapping genomic data from a scaffolded genome assembly to a telomere-to-telomere genome assembly. An optimized transformation protocol was developed that could consistently transform blunt-ended and DNA with overhangs and yielded up to 1000+ colony forming units per …
Effects Of 11-Kt And Prolactin On Gene Expression, Parental Care Behaviour And Immune Response In Male Bluegill Sunfish., Adriano A P Da Cunha
Effects Of 11-Kt And Prolactin On Gene Expression, Parental Care Behaviour And Immune Response In Male Bluegill Sunfish., Adriano A P Da Cunha
Electronic Thesis and Dissertation Repository
Parental care is very critical for reproduction in species that provide it. Hormones such as prolactin and androgens play a crucial role in parenting and reproductive behaviours. In mammals and birds, prolactin’s role in parental care is well-established; it stimulates milk production and stimulates attachment to newborns by its release in the preoptic area of the hypothalamus (POA), among other functions. Androgens, on the other hand, are commonly associated with male secondary sex characteristics, territoriality, and aggressiveness in mammals and birds. There are similar reports of prolactin and androgen effects in fish, but there are fewer studies. I investigated if …
Mouse Genotyping Array-Based Analysis Of Variation At Single Nucleotide Polymorphic Loci In The Context Of Macro- And Micro-Environments, Freda Qi
Electronic Thesis and Dissertation Repository
Comparing genetic variation between populations can provide insight into selective pressures in the environment. The Mouse Diversity Genotyping Array (MDGA) is a low-cost, high-throughput tool developed to capture and analyze variation at single nucleotide polymorphic (SNP) loci in inbred mouse strains. However, its ability to assess genetic variation in non-inbred mice and associate SNPs with the environment has not been extensively studied. First, SNPs between laboratory inbred and wild-caught mice were compared. No SNPs were associated with either macroenvironment. Instead, SNPs reflected genealogy and inbreeding strategies, consistent with the MDGA’s intended use. Second, mutations at SNP loci were compared between …