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Full-Text Articles in Genomics
Cpf1-Based Crispr Genome Editing In The Cyanobacterium N. Punctiforme, Soohan Woo
Cpf1-Based Crispr Genome Editing In The Cyanobacterium N. Punctiforme, Soohan Woo
University of the Pacific Theses and Dissertations
CRISPR systems have been growing in their utility and their application throughout the biological field as researchers continue to grow in their understanding of the relatively novel genome editing technology. However, despite the potential of CRISPR as a genome editing tool, the complexity of applying this technology to a specific organism calls for custom modifications to the system to improve its success rate. In this project, a CRISPR-Cpf1 system that can be effectively employed in the cyanobacterium Nostoc punctiforme was designed, with a focus on the hormogonium development of this species. Multiple plasmids containing the CRISPR system and targeting different …