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Full-Text Articles in Genetics and Genomics
Validation Of A New Rapid Hybridization Buffer For Fluorescence In Situ Hybridization With A Review On The Kinetics Of Dna Hybridization, Christine O'Connor
Validation Of A New Rapid Hybridization Buffer For Fluorescence In Situ Hybridization With A Review On The Kinetics Of Dna Hybridization, Christine O'Connor
Honors Scholar Theses
Abbott Molecular recently developed the rapid Vysis IntelliFISH Hybridization Buffer for use in fluorescence in situ hybridization (FISH) assays. The hybridization step in a standard FISH assay requires an overnight incubation, thus the turnaround time for a patient result is at least 24 hours. The IntelliFISH buffer was designed to reduce hybridization time of probes to target DNA to about two hours, allowing the entire assay to be performed in a single day. A review of methodologies and commercial products has highlighted the importance of hybridization kinetics to a successful FISH assay. The purpose of the research was to investigate …
Targeted Germ Cell Knockdown Of Xlr3 In Mus Musculus, Caity Miller
Targeted Germ Cell Knockdown Of Xlr3 In Mus Musculus, Caity Miller
Honors Scholar Theses
The X chromosome is rather distinct from autosomes due to the unique regulatory and functional characteristics it exhibits. Xlr3 is just one gene in a superfamily of highly related, and homologous genes found on the X chromosome in mice. Xlr3 is part of a complex, imprinted locus, of which the function is not well understood, although its protein product can be found in testis and oocyte. However, it is theorized that the region may be implicated in the progression of meiosis, due to localization of XLR3. This study sought to characterize the function of Xlr3 through the use of a …