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Full-Text Articles in Genetics and Genomics
Genomic And Microarray Analysis Of Aromatics Degradation In Geobacter Metallireducens And Comparison To A Geobacter Isolate From A Contaminated Field Site, Jessica E. Butler, Qiang He, Kelly P. Nevin, Zhili He, Jizhong Zhou, Derek R. Lovley
Genomic And Microarray Analysis Of Aromatics Degradation In Geobacter Metallireducens And Comparison To A Geobacter Isolate From A Contaminated Field Site, Jessica E. Butler, Qiang He, Kelly P. Nevin, Zhili He, Jizhong Zhou, Derek R. Lovley
Faculty Publications and Other Works -- General Biology
Background
Groundwater and subsurface environments contaminated with aromatic compounds can be remediated in situ by Geobacter species that couple oxidation of these compounds to reduction of Fe(III)-oxides. Geobacter metallireducens metabolizes many aromatic compounds, but the enzymes involved are not well known.
Results
The complete G. metallireducens genome contained a 300 kb island predicted to encode enzymes for the degradation of phenol, p-cresol, 4-hydroxybenzaldehyde, 4-hydroxybenzoate, benzyl alcohol, benzaldehyde, and benzoate. Toluene degradation genes were encoded in a separate region. None of these genes was found in closely related species that cannot degrade aromatic compounds. Abundant transposons and phage-like genes in …
On The Functions Of The H Subunit Of Eukaryotic Initiation Factor 3 In Late Stages Of Translation Initiation, Byung-Hoon Kim, Xue Cai, Justin N. Vaughn, Albrecht G. Von Arnim
On The Functions Of The H Subunit Of Eukaryotic Initiation Factor 3 In Late Stages Of Translation Initiation, Byung-Hoon Kim, Xue Cai, Justin N. Vaughn, Albrecht G. Von Arnim
Faculty Publications and Other Works -- General Biology
Background
The eukaryotic translation initiation factor 3 (eIF3) has multiple roles during the initiation of translation of cytoplasmic mRNAs. How individual subunits of eIF3 contribute to the translation of specific mRNAs remains poorly understood, however. This is true in particular for those subunits that are not conserved in budding yeast, such as eIF3h.
Results
Working with stable reporter transgenes in Arabidopsis thaliana mutants, it was demonstrated that the h subunit of eIF3 contributes to the efficient translation initiation of mRNAs harboring upstream open reading frames (uORFs) in their 5' leader sequence. uORFs, which can function as devices for translational regulation, …
Efficient Gene-Driven Germ-Line Point Mutagenesis Of C57bl/6j Mice, Edward J. Michaud, Cymbeline T. Culiat, Mitchell L. Klebig, Paul E. Barker, Kt Cain, Debra J. Carpenter, Lori L. Easter, Carmen M. Foster, Alysyn W. Gardner, Zy Guo, Kay J. Houser, Lori A. Hughes, Marilyn K. Kerley, Zhaowei Liu, Robert E. Olszewski, Irina Pinn, Ginger D. Shaw, Sarah G. Shinpock, Ann M. Wymore, Eugene M. Rinchik, Dabney K. Johnson
Efficient Gene-Driven Germ-Line Point Mutagenesis Of C57bl/6j Mice, Edward J. Michaud, Cymbeline T. Culiat, Mitchell L. Klebig, Paul E. Barker, Kt Cain, Debra J. Carpenter, Lori L. Easter, Carmen M. Foster, Alysyn W. Gardner, Zy Guo, Kay J. Houser, Lori A. Hughes, Marilyn K. Kerley, Zhaowei Liu, Robert E. Olszewski, Irina Pinn, Ginger D. Shaw, Sarah G. Shinpock, Ann M. Wymore, Eugene M. Rinchik, Dabney K. Johnson
Faculty Publications and Other Works -- General Biology
Background
Analysis of an allelic series of point mutations in a gene, generated by N-ethyl-N-nitrosourea (ENU) mutagenesis, is a valuable method for discovering the full scope of its biological function. Here we present an efficient gene-driven approach for identifying ENU-induced point mutations in any gene in C57BL/6J mice. The advantage of such an approach is that it allows one to select any gene of interest in the mouse genome and to go directly from DNA sequence to mutant mice.
Results
We produced the Cryopreserved Mutant Mouse Bank (CMMB), which is an archive of DNA, cDNA, tissues, and …
Fine Mapping In Tomato Using Microsynteny With The Arabidopsis Genome: The Diageotropica (Dgt) Locus, Kwangchul Oh, Kristine Hardeman, Maria G. Ivanchenko, Mary Ellard-Ivey, Andreas Nebenfüh, Tj White, Terri L. Lomax
Fine Mapping In Tomato Using Microsynteny With The Arabidopsis Genome: The Diageotropica (Dgt) Locus, Kwangchul Oh, Kristine Hardeman, Maria G. Ivanchenko, Mary Ellard-Ivey, Andreas Nebenfüh, Tj White, Terri L. Lomax
Faculty Publications and Other Works -- General Biology
Background
The Arabidopsis thaliana genome sequence provides a catalog of reference genes applicable to comparative microsynteny analysis of other species, facilitating map-based cloning in economically important crops. We have applied such an analysis to the tomato expressed sequence tag (EST) database to expedite high-resolution mapping of the Diageotropica (Dgt) gene within the distal end of chromosome 1 in tomato (Lycopersicon esculentum).
Results
A BLAST search of the Arabidopsis database with nucleotide sequences of markers that flank the tomato dgt locus revealed regions of microsynteny between the distal end of chromosome 1 in tomato, two regions of …