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Full-Text Articles in Entomology

Eristalis Tenax Movement Behavior In Response To Light, Temperature, And Food, Jeffery J. Zheng, Zdena M. Janderova, Jason D. Lang Jan 2024

Eristalis Tenax Movement Behavior In Response To Light, Temperature, And Food, Jeffery J. Zheng, Zdena M. Janderova, Jason D. Lang

Georgia Journal of Science

Drone flies, Eristalis tenax (Diptera: Syrphidae), are important generalist pollinators and visit flowers globally that range widely in color. The flies’ photoreceptors allow them to sense light wavelengths between 300-600 nm and E. tenax exhibit a positive phototactic response. To understand the effects of light on E. tenax movement, we conducted two-choice behavioral tests to determine their phototactic response to different wavelengths of light across the spectrum (ultraviolet to red light, plus full spectrum white light). The drone flies moved most and quickest toward sunlight, with almost twice the percentage of flies moving toward sunlight than toward black and …


Species Richness Of Moths In Parks Surrounded By Varying Levels Of ​Urbanization Around Nashville, Tennessee, Maxwell Stone, Allie Bennett Nov 2023

Species Richness Of Moths In Parks Surrounded By Varying Levels Of ​Urbanization Around Nashville, Tennessee, Maxwell Stone, Allie Bennett

Science University Research Symposium (SURS)

Moths can act as indicators of environmental wellness due to their pollution sensitivity and the complexity of biodiversity required to support their life cycles. Urbanization can impact the occurrence of moths in protected green spaces. Higher moth species richness was hypothesized to occur in parks surrounded by more rural areas. Three metropolitan parks were chosen in the Nashville area: downtown, within a suburban neighborhood, and in a rural area. Tree canopy cover and degree of impervious surfaces were assessed for each park and surrounding area using iTreeCanopy. Moths were attracted to a white sheet using a mercury vapor bulb, UV …


U.S. History, Science History, Women Scientists, History Of Entomology, Mentoring, Gender In Science, Nature Studies, Ecology, Cornell University, History Of Higher Education, Pat Munday Oct 2023

U.S. History, Science History, Women Scientists, History Of Entomology, Mentoring, Gender In Science, Nature Studies, Ecology, Cornell University, History Of Higher Education, Pat Munday

Interdisciplinary Arts and Sciences Articles

James G. Needham (1868-1958) was a professor of entomology at Cornell University from 1906 to 1936, and an active emeritus for about ten years thereafter. As a professor, mentored many women graduate students at Cornell, a group that included twenty-nine who took doctorates. As a scientist, he was a member of an extensive network that included many more women entomologists. These women were located throughout the United States and Canada. Some had been his students at Cornell, some were colleagues with whom he did fieldwork, and others were young women who, even though students at colleges other than Cornell, worked …


The Bellarmine Bee Bed: Organizing A Native Plant Garden Using Feedback From The Local Community, Kate Moran Apr 2023

The Bellarmine Bee Bed: Organizing A Native Plant Garden Using Feedback From The Local Community, Kate Moran

Undergraduate Theses

Animal pollinators are the cornerstone of healthy ecosystems. Their survival is essential for the persistence of entire food chains: from the flowers they cross-pollinate directly, to the animals who depend on those plants for nutrition. The establishment of pollinator gardens—particularly ones that consist of native plants—is an effective way to enhance their biodiversity, abundance, and well-being.

The main goal of this thesis is to construct a pollinator garden that maximizes the benefits for animal pollinators using feedback from local gardeners. A survey was used to gather information about the popularity and preferences of 40 flowering plants, and after analyzing the …


The Status Of Invasive Dusky Slug (Arion) Species In The Upper Great Lakes: A Molecular Approach, Olivia W. Hall Apr 2023

The Status Of Invasive Dusky Slug (Arion) Species In The Upper Great Lakes: A Molecular Approach, Olivia W. Hall

All NMU Master's Theses

I update the distribution of two cryptic slug species, Arion fuscus and Arion subfuscus, in the Upper Great Lakes of the United States using molecular identification methods. Arion fuscus has not previously been reported in the literature in this region, and Arion subfuscus has previously been reported as abundant. However, all previous distribution studies were determined using visual identification tools, which can result in misidentification between cryptic species. To molecularly re-examine these distribution maps, I used a mitochondrial ribosomal subunit 16S PCR amplification and subsequent SSpI and MfeI restriction enzyme digest to identify slug specimens. I collected slugs in …


Evolution Of A Genus Of Gall Wasp Kleptoparasites, Guerin E. Brown Mar 2023

Evolution Of A Genus Of Gall Wasp Kleptoparasites, Guerin E. Brown

2023 Midwest Ecology & Evolution Conference

Kleptoparasites do not directly parasitize their hosts but instead steal food and resources, reducing host fitness. Like direct parasites, kleptoparasites can be highly dependent on their hosts such that their evolutionary histories may be linked. Here, we study the evolution of a kleptoparasitic wasp genus, Synergus (Hymenoptera: Cynipidae: Synergini), in relation to their hosts, oak gall wasps (Hymenoptera: Cynipidae: Cynipini). Ovipositing oak gall wasps induce the formation of galls on oaks (Fagaceae) that provide food and shelter to one or more developing gall wasps. Galls induced by different gall wasp species are diverse in size, shape, color, and location …


Data And R Code For "Gut Transplants From Bees Fed An Antipathogenic Pollen Diet Do Not Confer Pathogen Resistance To Recipients", Rachel T. Yost, Alison E. Fowler, Lynn S. Adler Jan 2023

Data And R Code For "Gut Transplants From Bees Fed An Antipathogenic Pollen Diet Do Not Confer Pathogen Resistance To Recipients", Rachel T. Yost, Alison E. Fowler, Lynn S. Adler

Data and Datasets

Pollinators are threatened by diverse stressors, including microbial pathogens such as Crithidia bombi. Consuming sunflower pollen dramatically reduces C. bombi infection in the bumble bee Bombus impatiens, but the mechanism behind this medicinal effect is unclear. We asked whether diet mediates resistance to C. bombi through changes in the gut microbiome. We hypothesized that sunflower pollen changes the gut microbiome, which in turn reduces Crithidia infection. To test this, we performed a gut transplant experiment. We fed donor bees either a sunflower pollen treatment or buckwheat pollen as a control treatment, and then inoculated recipient bees with homogenized guts …


Exploring The Relationships Between South Texas Northern Bobwhite Populations And Cecal Worms Via System Dynamics, Nicole J. Traub, Benjamin L. Turner, Leonard A. Brennan, Alan M. Fedynich Sep 2022

Exploring The Relationships Between South Texas Northern Bobwhite Populations And Cecal Worms Via System Dynamics, Nicole J. Traub, Benjamin L. Turner, Leonard A. Brennan, Alan M. Fedynich

National Quail Symposium Proceedings

Community ecology historically focused on plants and free-living organisms; however, problems such as defining habitat boundaries and obtaining adequate sample sizes arise when evaluating such communities. The unique nature of host-helminth systems allows parasite community ecologists to avoid these problems when testing ecological hypotheses. Unlike free-living communities that have artificially constructed boundaries, parasite communities have well-defined unambiguous boundaries within host individuals. Due to the inherently complex and dynamic nature of ecological systems, traditional experimental methods often require expensive, long-term trials beyond investigators’ time and resource budgets. Conversely, a system dynamics approach facilitates learning about such systems via simulation of ecosystem …


Effects Of Mass Death On Community Structure And Ecosystem Function, Abby Kimpton Jones Aug 2022

Effects Of Mass Death On Community Structure And Ecosystem Function, Abby Kimpton Jones

Theses and Dissertations

Death and decomposition are natural processes that are generally well-understood. However, large events of death, such as mass mortality events (MMEs) are increasing in frequency and their impacts on the ecosystem are largely unknown. These events may have both bottom-up effects from increased nutrient input as well as top-down effects from loss of an ecological functional group by the affected population. Different functional MMEs may result in different top-down effects, creating cascading effects. In Chapter 1, I test the hypothesis that scavenger and herbivore simulated MMEs generate novel bottom-up and top-down effects. Results indicate that MMEs have a significant effect …


Plant Neighbor Interactions Of A Native Goldenrod Impact Abundance And Composition Of Pollinator Communities, Emma P. Kruse May 2022

Plant Neighbor Interactions Of A Native Goldenrod Impact Abundance And Composition Of Pollinator Communities, Emma P. Kruse

Chancellor’s Honors Program Projects

No abstract provided.


Mark-Recapture Study And Habitat Assessment For The Northern Metalmark Butterfly, Calephelis Borealis (Lepidoptera: Riodinidae), Weston J. Henry, Kristian S. Omland, Henry Frye, Wagner L. David Feb 2022

Mark-Recapture Study And Habitat Assessment For The Northern Metalmark Butterfly, Calephelis Borealis (Lepidoptera: Riodinidae), Weston J. Henry, Kristian S. Omland, Henry Frye, Wagner L. David

EEB Articles

Background: The northern metalmark (Calephelis borealis), is an exceedingly local, globally rare butterfly that is declining across the Midwestern and Northeastern USA. The principal stressors driving colony losses include afforestation and invasive plants that crowd out its larval hostplant (Packera ovata) and nectar resources.

Aims/Methods: To better understand its declines and guide restoration efforts, we 1) performed a mark-recapture study in Connecticut to document population trends where we were actively managing vegetation; 2) conducted a range-wide survey for evidence of phylogeographic structure, using cytochrome oxidase (CO1); 3) investigated abundance determinants of its larval foodplant, Packera ovata …


Diversity And Drivers Of Oribatid Mites (Acari: Oribatida) In Boreal Peatlands, Carlos Rafael De Araujo Barreto Nov 2021

Diversity And Drivers Of Oribatid Mites (Acari: Oribatida) In Boreal Peatlands, Carlos Rafael De Araujo Barreto

Electronic Thesis and Dissertation Repository

Boreal peatlands are important ecosystems for carbon cycling, storing 1/3 of the world’s terrestrial carbon in only ~3% of the globe, making them a key component of potential mitigation strategies in response to global climate warming. Experiments have shown that warming can affect plant and microbial communities in ways that potentially shift peatlands from carbon sinks to sources. Soil food webs, including the microarthropod community, are key in carbon cycling but are relatively understudied both in peatlands and under experimental warming. My research capitalized on a large-scale experimental field manipulation of warming in two contrasting peatland sites in Northern Ontario, …


Interspersed Denuded Zone (Idz): How Patchy Leaf Litter Dynamics In A Buckthorn-Invaded Urban Woodland Can Affect Microarthropod Species Richness, Angela Stenberg Aug 2021

Interspersed Denuded Zone (Idz): How Patchy Leaf Litter Dynamics In A Buckthorn-Invaded Urban Woodland Can Affect Microarthropod Species Richness, Angela Stenberg

DePaul Discoveries

Biodiversity loss may serve as a key diagnostic of the Anthropocene. An important driver of this loss is by means of invasive species. In this study of a forest preserve in Chicago, Illinois we examined Interspersed Denuded Zones (IDZs for short), which are areas of patchy leaf litter in invaded forests caused in this case by the rapid decomposition of litter from buckthorn (Rhamnus cathartica). We characterized the leaf litter mass in IDZs and enumerated litter-inhabiting microarthropod populations. We found that plots of high buckthorn density are associated with IDZs: there was significantly less leaf litter mass in …


Correlation Between Abundance Of Fossils In Harvester Ant Nest And Proximity Of Fossil Sites, Hayeong Woo Apr 2021

Correlation Between Abundance Of Fossils In Harvester Ant Nest And Proximity Of Fossil Sites, Hayeong Woo

Campus Research Day

Harvester ants have an interesting behavior of bone collecting. Therefore, harvester ant nests that are positioned around the fossil sites contain various fossil fragments. If there is a statistically significant correlation between the abundance of fossil fragments and the distance of the ant nests from the main fossil sites, ant nests can potentially be used to predict the proximity of the main fossil sites.


Insect Hotels: A Biological Remedy For The Survivability Of Insects And The Human Species, Lewis University Apr 2021

Insect Hotels: A Biological Remedy For The Survivability Of Insects And The Human Species, Lewis University

Come Clean, Go Green Competition

No abstract provided.


The Reconstruction Of Hatchery Creek: Effects On Macroinvertebrate And Fish Community Dynamics, Patrick Vrablik Jan 2021

The Reconstruction Of Hatchery Creek: Effects On Macroinvertebrate And Fish Community Dynamics, Patrick Vrablik

Murray State Theses and Dissertations

Hatchery Creek is a restored stream in Jamestown, KY that drains Wolf Creek National Fish Hatchery. The previous degraded channel of Hatchery Creek caused large sediment plumes in the Lower Cumberland River and was restored to decrease sediment loss and provide the opportunity for a self-sustaining trout population. I predicted that the increased amount of habitat would increase taxa richness, abundance and biomass of macroinvertebrates. Macroinvertebrates were monitored in three periods; the degraded period, a one-year recovery period, and a restored period using surber and kicknet samples in order to determine biomass, abundance, diversity, taxa richness, and macroinvertebrate biotic index. …


Macroinvertebrate Responses To Hydrological Variation In Experimental Wetlands., Sergio A. Sabat-Bonilla Jan 2021

Macroinvertebrate Responses To Hydrological Variation In Experimental Wetlands., Sergio A. Sabat-Bonilla

Electronic Theses and Dissertations

Predicted increases in the frequency of intense storms and periods of severe drought due to climate change represent a threat to wetland macroinvertebrate communities through alterations to the hydrological regime. I used experimental ponds to assess the effects of water permanence (i.e., duration of flooding) on the communities of aquatic macroinvertebrates. I predicted that permanent ponds would harbor higher diversity of longer-lived taxa whereas temporary ones will favor colonization by quick turnover, short-lived taxa and support lower consumer diversity. Results show differences in macroinvertebrate communities between permanent and temporary ponds can be mostly explained by hydrology and the amount of …


Effects Of Insect Opportunists On A Four-Level Trophobiotic System Involving Nectar-Producing Galls Of The Cynipid Wasp Disholcaspis Quercusmamma (Hymenoptera: Cynipidae), Stephanie L. Smith Jan 2020

Effects Of Insect Opportunists On A Four-Level Trophobiotic System Involving Nectar-Producing Galls Of The Cynipid Wasp Disholcaspis Quercusmamma (Hymenoptera: Cynipidae), Stephanie L. Smith

Masters Theses

The induction of plant galls is considered an adaptive life history trait found in many insect groups. The formation of galls provides several advantages to the gall maker, such as enhanced nutrition, favorable microclimate, and protection from natural enemies, including parasitoids, inquilines, and predators. Order Hymenoptera has many gall-making species, belonging to the gall wasp family Cynipidae. As an extended phenotype of the gall makers, some galls exhibit very sophisticated adaptive mechanisms involving multilevel species interactions. In particular, the oak galls of the Cynipid species Disholcaspis quercusmamma, found in much of Illinois, produce a palatable, sugary nectar-like secretion, attracting …


Bee Diversity Of Three Appalachian Shale Barren Sites, Olivia C. Latham Jan 2020

Bee Diversity Of Three Appalachian Shale Barren Sites, Olivia C. Latham

Theses and Dissertations

Insect pollination is vital to ecosystem function. However, climate change, habitat loss, pesticides, and a variety of other anthropogenic sources are contributing to a decline in pollinator diversity. Fragile small ecosystems with a high composition of specialized plant species that rely on specific pollinators such as Appalachian shale barrens, are especially at risk of losing biodiversity. This study combines the use of active sweep net sampling of endemic shale barren forbs and passive trap methods over the course of a bloom season (April-August) in three barren sites to identify bee community populations and visitation relationships between pollinator species and endemic …


Effects Of Abiotic Factors On Host Plant Quality And Community Structure Of The Parasitoid Guild That Attacks Asphondylia Borrichiae Midges On Borrichia Frutescens, Kevin Orta Jan 2020

Effects Of Abiotic Factors On Host Plant Quality And Community Structure Of The Parasitoid Guild That Attacks Asphondylia Borrichiae Midges On Borrichia Frutescens, Kevin Orta

UNF Graduate Theses and Dissertations

Asphondylia borrichiae is a gall-inducing insect that attacks its ancestral host plant Borrichia frutescens: through ovipositional mistakes it has acquired two additional hosts, Iva frutescens and Iva imbricata. Oviposition results in the formation of a gall, a tumor-like growth of plant tissue within which immature A. borrichiae develop. During development, immature A. borrichiae, are attacked by a suite of four generalist hymenopteran parasitoids. Of these, Galeopsomyia haemon and Torymus umbilicatus are facultative hyperparasitoids, more common and exhibit non-random attack patterns. In the present study, soil quality was manipulated through bi-weekly application of either ammonium nitrate fertilizer or sodium …


Heat For The Masses: Thermal Ecology Of The Western Tent Caterpillar, Victoria Dahlhoff Jan 2020

Heat For The Masses: Thermal Ecology Of The Western Tent Caterpillar, Victoria Dahlhoff

Graduate Student Theses, Dissertations, & Professional Papers

A unique feature of some gregarious, colonial insects is their ability to create external structures that alter environmental conditions for the entire (often family) group. A combination of physical alteration of local microhabitats and behavioral thermoregulation allows many of these animals to actively control their body temperatures, which allows them to regulate energy use and metabolism in variable thermal environments. Here I describe mechanisms of microhabitat modification and thermal regulation in the western tent caterpillar, Malacosoma californicum pluviale. Tent caterpillars build communal silk tents, whose temperatures can rise substantially above ambient air temperature. I experimentally manipulated colony sizes and examined …


The Evolution Of Spinosad Resistance In Colorado Potato Beetles (Leptinotarsa Decemlineata), Coby Michael Klein Sep 2019

The Evolution Of Spinosad Resistance In Colorado Potato Beetles (Leptinotarsa Decemlineata), Coby Michael Klein

Dissertations, Theses, and Capstone Projects

Colorado potato beetles (Leptinotarsa decemlineata) are a major pest of cultivated potato plants worldwide. They are well-known for their ability to rapidly evolve resistance to all major classes of pesticides. Defoliation of potato plants by L. decemlineata can reduce potato yields by a considerable margin. The damage done by resistant beetles is steep and much research is focused on developing new chemical controls, especially those derived from naturally occurring compounds. Spinosad is a relatively new natural product insecticide, introduced approximately a decade ago, suitable for use in organic farming. Potato beetles on Long Island, NY developed very strong …


Integrated Pest Management In The Academic Small Greenhouse Setting: A Case Study Using Solanum Spp. (Solanaceae)., Daniel S. Hayes, Ingrid E. Jordon-Thaden, Jason T. Cantley, Angela J. Mcdonnell, Christopher T. Martine Aug 2019

Integrated Pest Management In The Academic Small Greenhouse Setting: A Case Study Using Solanum Spp. (Solanaceae)., Daniel S. Hayes, Ingrid E. Jordon-Thaden, Jason T. Cantley, Angela J. Mcdonnell, Christopher T. Martine

Faculty Journal Articles

Premise

Botanical faculty and staff at academic institutions are often tasked with establishing and/or caring for plant collections held in small greenhouse facilities. Once plants are in place, an especially acute challenge is managing plant pest/pathogen populations. Integrated pest management (IPM) approaches are an excellent option, but few examples exist in the literature of successful programs that have been developed in academic small greenhouse settings.

Methods and Results

Over several years, we developed an IPM program for two small research greenhouses on the campus of a primarily undergraduate institution where hundreds of plants have been grown for studies in the …


Species-Area Model Predicting Diversity Loss In An Artificially Flooded Cave In Brazil, Rodrigo L. Ferreira, Thais G. Pellegrini Jul 2019

Species-Area Model Predicting Diversity Loss In An Artificially Flooded Cave In Brazil, Rodrigo L. Ferreira, Thais G. Pellegrini

International Journal of Speleology

Subterranean environments are poorly known regarding many ecological aspects, such as community structure and its response to different disturbances. To estimate the effects of ground area lost in a limestone cave community in Southeastern Brazil, the invertebrate fauna was sampled before 76% of the cave floor was submerged by the filling of a hydroeletric power plant reservoir. Then, a 2-year monitoring was conducted. A species-area curve based on empiric data was constructed and the z-value of the species-area equation was calculated, what allowed estimating the expected cave richness after flooding comparing with data obtained during the monitoring. The results support …


Pollinator Visitation Frequency Associated With Native And Non-Native Plants In A Mid-Atlantic Piedmont (Usa) Urban Garden, Nicholas J. Ruppel, Saunders M. Riley, Ellis D. Mumford, Barbara L. Swedo Jul 2019

Pollinator Visitation Frequency Associated With Native And Non-Native Plants In A Mid-Atlantic Piedmont (Usa) Urban Garden, Nicholas J. Ruppel, Saunders M. Riley, Ellis D. Mumford, Barbara L. Swedo

Virginia Journal of Science

The recent focus on the importance of native plants and their pollinators has highlighted the critical role of local species in their natural environment. As urban encroachment, climate change, and invasive species continues to threaten native habitats, it is increasingly important to promote the use of local green spaces as refugia for native plants and their pollinators. The aim of this project, therefore, was to identify and assess the visitation frequency of insect pollinators associated with an urban setting within the Piedmont region of Virginia, and compare their association with native versus closely-related but non-native summer-flowering plants. Several modes of …


Recovery Of Domestic Behaviors By A Parasitic Ant (Formica Subintegra) In The Absence Of Its Host (Formica Subsericea), Amber Nichole Hunter May 2019

Recovery Of Domestic Behaviors By A Parasitic Ant (Formica Subintegra) In The Absence Of Its Host (Formica Subsericea), Amber Nichole Hunter

MSU Graduate Theses

Dulosis is a type of social parasitism in which a parasitic ant has become dependent on captured workers (hosts) of a closely related species. Dulosis can be either facultative or obligate depending on the degree of dependence on host workers. As parasites become more specialized over evolutionary time, their domestic abilities degenerate until they become unable to survive without the host. However, the ‘lost’ behaviors may display some degree of recovery when host workers are unavailable to do the tasks. The Formica sanguinea group consists of 12 species of parasitic ants, which are all traditionally considered to be facultative parasites. …


Lingering Impacts Of Hurricane Hugo On Rhizophora Mangle (Red Mangrove) Population Genetics On St. John, Usvi, Paul Ax Bologna, James J. Campanella, Dena J. Restaino, Zachary A. Fetske, Matthew Lourenco, John V. Smalley Apr 2019

Lingering Impacts Of Hurricane Hugo On Rhizophora Mangle (Red Mangrove) Population Genetics On St. John, Usvi, Paul Ax Bologna, James J. Campanella, Dena J. Restaino, Zachary A. Fetske, Matthew Lourenco, John V. Smalley

Marine Biology and Coastal Sciences Faculty Scholarship and Creative Works

Stochastic events can have catastrophic effects on island populations through a series of genetic stressors from reduced population size. We investigated five populations of red mangrove (Rhizophora mangle) from St. John, USVI, an UNESCO Biosphere Reserve, which were impacted by Hurricane Hugo in 1989. Our goal was to determine diversity and to ascertain potential population bottlenecks two decades after the event. With the lowest observed heterozygosity, highest inbreeding coefficient, and evidence of a major bottleneck, our results demonstrated that the Great Lameshur mangroves, devastated by Hurricane Hugo, were the least diverse stand of trees. The other four populations from St. …


Spatial And Temporal Distribution Of The Forensically Significant Blow Flies Of Los Angeles County, California, United States (Diptera: Calliphoridae), Royce T. Cumming Apr 2019

Spatial And Temporal Distribution Of The Forensically Significant Blow Flies Of Los Angeles County, California, United States (Diptera: Calliphoridae), Royce T. Cumming

School of Natural Resources: Dissertations, Theses, and Student Research

Forensic entomology although not a commonly used discipline in the forensic sciences, does have its niche and when used by investigators is respected in crinimolegal investigations (Greenberg and Kunich, 2005). With many species of forensically significant insects being regionally specific, it is often difficult for forensic entomologists to as confidently translate regionally specific studies across drastically differing geographic regions (Brundage, et al., 2011).

The purpose of this study is to help create a better temporal and geographic distributional understanding of the blow fly species present in Los Angeles County, California, United States. Twenty-five locations from four ecoregions (coastal mountains, urban, …


Index Apr 2019

Index

Bulletin of the University of Nebraska State Museum

No abstract provided.


Density And Mass Effect On The Development Of Phormia Regina, Brandon H. Strauss Apr 2019

Density And Mass Effect On The Development Of Phormia Regina, Brandon H. Strauss

School of Natural Resources: Dissertations, Theses, and Student Research

Forensic entomology is the application of the study of arthropods to the criminal justice system. This is primarily done through the development of a post mortem interval (PMI) based the insect evidence present. A practitioner must be able to determine the age of the insect through temperature data. One factor influencing the temperature dependent development is gregarious behavior. Current literature describes a faster development rate due to an increase in feeding efficiency and temperatures produced by this aggregate. However, there is very little literature defining a minimum number needed to induce this effect and little to none on it for …