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Full-Text Articles in Ecology and Evolutionary Biology

Efficacy Of Non-Lethal Molecular Methods In Elucidating Distribution Of Gray Treefrog Complex (Hyla Chrysoscelis/Versicolor) In Kansas, Nora K. Lazerus Jan 2022

Efficacy Of Non-Lethal Molecular Methods In Elucidating Distribution Of Gray Treefrog Complex (Hyla Chrysoscelis/Versicolor) In Kansas, Nora K. Lazerus

Master's Theses

Globally, amphibians are the most imperiled vertebrate taxa in part because they rely on both aquatic and terrestrial ecosystems. Specifically, their permeable skin makes them uniquely susceptible to habitat degradation and alteration. Cope’s Gray Treefrog (Hyla chrysoscelis) and the Gray Treefrog (Hyla versicolor) are a diploid-tetraploid, morphologically indistinguishable sister pair of cryptic anurans native to Kansas. Since 1987, the distribution of gray treefrogs in Kanas has extended west but the status of each species in the complex in Kansas is not known beyond its documented combined western expansion. Currently, species identification cannot be determined by nonlethal …


Parasitaemia Data And Molecular Characterization Of Haemoproteus Catharti From New World Vultures (Cathartidae) Reveals A Novel Clade Of Haemosporida, Michael J. Yabsley, Ralph E.T. Vanstreels, Ellen S. Martinsen, Alexandra G. Wickson, Amanda E. Holland, Sonia M. Hernandez, Alec T. Thompson, Susan L. Perkins, Christopher A. Lawrence Bryan, Christopher A. Cleveland, Emily Jolly, Justin D. Brown, Dave Mcruer, Shannon Behmke, James C. Beasley Jan 2018

Parasitaemia Data And Molecular Characterization Of Haemoproteus Catharti From New World Vultures (Cathartidae) Reveals A Novel Clade Of Haemosporida, Michael J. Yabsley, Ralph E.T. Vanstreels, Ellen S. Martinsen, Alexandra G. Wickson, Amanda E. Holland, Sonia M. Hernandez, Alec T. Thompson, Susan L. Perkins, Christopher A. Lawrence Bryan, Christopher A. Cleveland, Emily Jolly, Justin D. Brown, Dave Mcruer, Shannon Behmke, James C. Beasley

Faculty & Staff Scholarship

Background: New World vultures (Cathartiformes: Cathartidae) are obligate scavengers comprised of seven species in five genera throughout the Americas. Of these, turkey vultures (Cathartes aura) and black vultures (Coragyps atratus) are the most widespread and, although ecologically similar, have evolved differences in morphology, physiology, and behaviour. Three species of haemosporidians have been reported in New World vultures to date: Haemoproteus catharti, Leucocytozoon toddi and Plasmodium elongatum, although few studies have investigated haemosporidian parasites in this important group of species. In this study, morphological and molecular methods were used to investi- gate the epidemiology and molecular biology of haemosporidian parasites of …


Molecular Evolution And Phylogeography Of Mitochondrial Dna Cytochrome B Gene In Southern California Santa Ana Speckled Dace (Rhinichthys Osculus), Pia Marie Vanmeter Jun 2017

Molecular Evolution And Phylogeography Of Mitochondrial Dna Cytochrome B Gene In Southern California Santa Ana Speckled Dace (Rhinichthys Osculus), Pia Marie Vanmeter

Electronic Theses, Projects, and Dissertations

In this study, I sequenced the mitochondrial cytochrome b gene to elucidate the extent and pattern of genetic variations among and within populations of Rhinichthys osculus (Santa Ana Speckled Dace) found in the different watersheds in Southern California, Central California Coast and Eastern California Desert. I described and analyzed the structural characteristics and pattern of base sequence substitutions in the cytochrome b gene to understand the molecular evolution of the gene. The Phylogenetic analyses showed that the Southern California Santa Ana Speckled dace is a distinct population from the Central California Coast dace population and Eastern California Desert dace population, …


Phylogenetic Analysis Of Blacknose Dace (Rhinichthys) In West Virginia Streams, Samantha Taylor Jan 2011

Phylogenetic Analysis Of Blacknose Dace (Rhinichthys) In West Virginia Streams, Samantha Taylor

Theses, Dissertations and Capstones

Blacknose dace (Rhinichthys) are one of the most common cyprinid fishes in eastern North America. They also have been a topic of debate for over 30 years because morphology-based systematics has failed to clearly define their taxa. Taxonomists classify the complex into two species and one subspecies: the eastern form, R. atratulus atratulus; and the western form R. obtusus obtusus, and southern form R. obtusus meleagris. This research uses the mitochondrial cytochrome b gene and genomic RAG 2 gene in a phylogenetic analysis to help clarify species relations according to differences between each current species. Maps have been created to …


Identification Of Eurycea Using Cytochrome B, Karis A. Myers Dec 2009

Identification Of Eurycea Using Cytochrome B, Karis A. Myers

Senior Honors Theses

Genomic sequencing is a powerful tool that has many applications for research, one of which is in the field of taxonomy and the identification of species. This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. The Polymerase Chain Reaction is used to amplify a target DNA sequence, and the protocol for this procedure must be optimized for the specific sequence of target DNA. Primers must also be designed and modified for …


Speciation Within Bonneted Bats (Genus Eumops): The Complexity Of Morphological, Mitochondrial, And Nuclear Data Sets In Systematics, Molly M. Mcdonough, Loren K. Ammerman, Robert M. Timm, Hugh H. Genoways, Peter A. Larsen, Robert J. Baker Jan 2008

Speciation Within Bonneted Bats (Genus Eumops): The Complexity Of Morphological, Mitochondrial, And Nuclear Data Sets In Systematics, Molly M. Mcdonough, Loren K. Ammerman, Robert M. Timm, Hugh H. Genoways, Peter A. Larsen, Robert J. Baker

University of Nebraska State Museum: Mammalogy Papers

We phylogenetically analyze 705 base pairs of the cytochrome-b gene and 351 amplified fragment length polymorphism (AFLP) bands from populations of the karyotypically variable Wagner’s bonneted bat, Eumops glaucinus, and the Florida bonneted bat, Eumops floridanus (Chiroptera: Molossidae). Three karyotypes have been documented across the range of E. glaucinus, and we report that the karyotype from Cuba is morphologically similar to that from Jamaica. A 4th karyotype is present in specimens from western Ecuador. Three distinct lineages are present in both the cytochrome-b and AFLP trees. One lineage is restricted to western Ecuador and exhibits cytochrome-b …


A Phylogeny Of Genera Of The Fish Family Nemipteridae (Percoidei) Based On The Cytochrome B Gene, Denise R. Sliter Oct 2004

A Phylogeny Of Genera Of The Fish Family Nemipteridae (Percoidei) Based On The Cytochrome B Gene, Denise R. Sliter

Biological Sciences Theses & Dissertations

The species level taxonomy of the Nemipteridae is well understood but existing morphological data is insufficient to hypothesize a phylogeny of the five genera of this family of percoid fishes. Furthermore, there are three distinct and widely disparate habitat requirements within nemipterids and little is known how these evolved. Species in the genera Pentapodus, Scaevius and Scolopsis are typically found around shallow reefs. Nemipterus species are also found in shallow-water, but they inhabit strictly soft sediment bottoms. Parascolopsis is the only deep-water genus and its species are also found over soft sediments. Complete cytochrome b gene sequences (1140 base …