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Full-Text Articles in Cell Biology
Osteoinductivity Of Demineralized Bone: A Quantitative In Vitro Assessment, Yihong Zheng
Osteoinductivity Of Demineralized Bone: A Quantitative In Vitro Assessment, Yihong Zheng
Biological Sciences Theses & Dissertations
Acid demineralized bone (DMB) implanted in extraosseous sites induces bone formation. In vivo studies have shown that DMB stimulates the differentiation of mesenchymal cells into osteogenic cells. In this study an in vitro system was developed to demonstrate the osteoinductivity of DMB quantitatively. This in vitro working system was used to assess the osteoinductivity of variously processed DMB products as a means to evaluate their processing protocols. A human dermal fibroblastic (HDF) cell line was initiated from tissue explants as a working model, along with a human periosteal (HPO) cell line as a control. Ground DMB processed by LifeNet Transplant …
Fertilization And Mouse Embryo Development In The Presence Of Midazolam, Maria Leavitt
Fertilization And Mouse Embryo Development In The Presence Of Midazolam, Maria Leavitt
Biological Sciences Theses & Dissertations
The development of mouse embryos in vitro has provided a unique opportunity for the study of cellular differentiation and the influence of various agents on differentiation. Midazolam is administered for conscious sedation in the recovery of human ova. In the Norfolk IVF Program, midazolam is found in varying concentrations in follicular fluid. Mouse embryo formation and development has been used to test midazolam's effect on fertilization and early development as a model of human in vitro fertilization and development. Midazolam' s effects were studied using varying doses of midazolam on (1) in vivo fertilization and subsequent cell division, and (2) …