Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Biology (5)
- Neuroscience and Neurobiology (3)
- Biochemistry, Biophysics, and Structural Biology (2)
- Developmental Biology (2)
- Molecular Biology (2)
-
- Ecology and Evolutionary Biology (1)
- Engineering (1)
- Engineering Science and Materials (1)
- Environmental Microbiology and Microbial Ecology (1)
- Evolution (1)
- Mechanics of Materials (1)
- Medical Sciences (1)
- Medicine and Health Sciences (1)
- Microbiology (1)
- Neurosciences (1)
- Plant Pathology (1)
- Plant Sciences (1)
- Institution
Articles 1 - 11 of 11
Full-Text Articles in Cell Biology
Encapsulation And Culture Of Plant Protoplasts In Hydrogel Microspheres Using Droplet Microfluidics, Matthew Grasso
Encapsulation And Culture Of Plant Protoplasts In Hydrogel Microspheres Using Droplet Microfluidics, Matthew Grasso
Graduate College Dissertations and Theses
Plant morphogenesis requires the coordinated growth between cells and their neighbors. This coordination is regulated by internal and external signals that are both physical and chemical. It is understood that both mechanical and molecular signals are involved in the regulation of plant morphogenesis however, how mechanical signals induce molecular signals and vice versa is not. This is due to a lack of research tools for manipulating and measuring the mechanical environment of growing plant cells. Such tools would ideally provide individual plant cells with mechanically tunable physical microenvironments. This may allow changes in the cells physical environment to be linked …
Dissecting The Cellular Control Of Septin Organization In A Global Cereal Killer, Nawaraj Dulal
Dissecting The Cellular Control Of Septin Organization In A Global Cereal Killer, Nawaraj Dulal
Graduate Theses and Dissertations
Rice blast disease, caused by the filamentous fungus Magnaporthe oryzae, destroys sufficient rice each year to feed 60 million people, and is a serious threat to global food security. A wheat-adapted lineage of M. oryzae now poses threat to global wheat production. Rice blast disease is currently controlled using limited fungicides, and the emergence of fungicide resistance within M. oryzae populations is a growing concern. There is a pressing need to identify new classes of fungicides to control the disease, which requires better understanding of the basic biology of the pathogen. To establish disease, M. oryzae forms a specialized dome …
Characterization Of Microtubule Organizing Centers In The Genus Protostelium, Including Evolutionary Implications, Ethan Taylor Ozment
Characterization Of Microtubule Organizing Centers In The Genus Protostelium, Including Evolutionary Implications, Ethan Taylor Ozment
Graduate Theses and Dissertations
Microtubule organizing centers (MTOCs) are cellular regions of microtubule nucleation. The best known MTOCs are those associated with the centrosome, but several non-centrosomal MTOCs are known in eukaryotes, especially in land plants. MTOCs are poorly characterized across the breadth of amoebozoan diversity, but are well-known in certain amoebozoan lineages, including the genus of protosteloid slime molds Protostelium. The structure of the MTOC is known for two non-ciliated species, P. nocturnum and P. mycophaga, as well as P. aurantium, which can reversibly become ciliated under appropriate conditions. P. nocturnum and P. mycophaga have acentriolar centrosomal MTOCs while P. aurantium has a …
Molecular Mechanism Of Neurodegeneration Induced By 4-Nonylphenol, Michelle Alejandra Aranda
Molecular Mechanism Of Neurodegeneration Induced By 4-Nonylphenol, Michelle Alejandra Aranda
Open Access Theses & Dissertations
Neurodegeneration, a progressive loss of nerve cells, occurs in many neurological disorders, including Alzheimer's disease (AD) and Parkinson's diseases (PD), as well as in dementia. The pathogenesis of these diseases is unknown, and recent evidence suggests that environmental factors, which act as endocrine-disrupting compounds (EDCs) could play a significant role in developing the disease process. 4-Nonylphenol (4-NP), an EDC, and a ubiquitous environmental toxin has been shown to affect brain development and may cause neurodegeneration. 4-NP is produced in large quantities in the U.S. and used as raw materials for making detergents, pesticides, plastics, paints, cosmetics, rubber, and other industrial/household …
4-Nonylphenol Induces Neurodegeneration By Altering Cytoskeleton, Cynthia Carreon
4-Nonylphenol Induces Neurodegeneration By Altering Cytoskeleton, Cynthia Carreon
Open Access Theses & Dissertations
4-nonylphenol (4-NP), an endocrine-disrupting compound (EDC), has been shown to affect brain development and may cause neurodegeneration. In the environment, 4-NP arises as a degradation product of alkylphenol polyethoxylates, compounds widely used as nonionic surfactants in commercial production, as well as in herbicides, pesticides, polystyrene plastics, and paints and has been shown to undergo a high level of accumulation in biological tissues. However, the mechanism by which 4-NP exerts its effect is not understood. Recent results from our laboratory indicate that Gβγ, an important component of the G protein-signaling pathway, induces neuronal outgrowth and differentiation by modulating microtubule (MT) assembly, …
A Novel Method For Studying Gene Regulatory Elements In Vivo Reveals Essential Roles For Intron Splicing In Neurofilament Gene Expression, Chen Wang
Legacy Theses & Dissertations (2009 - 2024)
Axon outgrowth requires coordinated expression of critical cytoskeletal genes in response to extracellular cues. The spatial and temporal expression of these genes is regulated transcriptionally and posttranscriptionally. Such regulation is largely mediated through the activity of cis-regulatory elements within the cytoskeletal DNAs and RNAs, which recruit specific sets of trans-factors such as DNA- and RNA-binding proteins, as well as microRNAs. To identify the functionally active cis-elements, characterize their dynamically changing sets of trans-factors, and investigate the association between the two sets of regulators in the context of an intact developing nervous system, one needs to combine appropriate in vivo strategies …
Subcellular Analysis Of The Disulfide Proteome In P66shc Expressing Nerve Cells, Tyler Cann
Subcellular Analysis Of The Disulfide Proteome In P66shc Expressing Nerve Cells, Tyler Cann
Electronic Thesis and Dissertation Repository
The longevity associated protein p66Shc has been suggested to regulate organismal lifespan through initiation of apoptotic pathways. Following stress-induced translocation into the mitochondria, p66Shc promotes increased reactive oxygen species (ROS) production and triggers poorly defined downstream signaling events that lead to decreased cell viability. Protein disulfide bonding has recently emerged as aROSdependent post-translational modification that regulates protein function and signaling processes. Using the mouse hippocampal HT-22 cell line, I sought to determine the changes in the disulfide proteome associated with p66Shc mediatedROSproduction. Through Redox 2D-SDSPAGEanalysis of mitochondrial and cytosolic extracts, redox sensitive proteins altered by p66Shc mediatedROSformation were identified. Of …
Internalization Of F-Actin Monomers Into 3t3 Fibroblasts Via Thermal Inkjet Printing For Investigation Of Cytoskeleton Incorporation And Mechanics, Stephen Shuford
Internalization Of F-Actin Monomers Into 3t3 Fibroblasts Via Thermal Inkjet Printing For Investigation Of Cytoskeleton Incorporation And Mechanics, Stephen Shuford
All Theses
ABSTRACT
We will review the conversion of a standard thermal inkjet printer into a bioprinting system and the effects of printing F-actin monomers with cells. The use of any printing system along with biological material or for biological or medical use has been termed bioprinting. Bioprinting has been used in vascular grafts, scaffold design, gene transfection, micro patterning and many other applications and is very diverse. Specifically we will look at the internalization of F-actin monomers into 3T3 fibroblasts as a result of cell membrane disruption from thermal inkjet printing. If the actin monomers were internalized and then incorporated into …
Molecular Mechanisms Of Cell Migration Inhibition By Synthetic Triterpenoids, Ciric Chi Wing To
Molecular Mechanisms Of Cell Migration Inhibition By Synthetic Triterpenoids, Ciric Chi Wing To
Electronic Thesis and Dissertation Repository
Cell migration is an important mediator of cancer metastasis and invasion, which is responsible for 90% of cancer-related premature deaths in Canada. Synthetic triterpenoids are a class of promising anti-cancer compounds that have shown considerable efficacy in targeting various cellular functions including apoptosis, growth, inflammation and cytoprotection in both cell culture and animal tumor models. However, their effect on cell migration, an important event in metastasis, remains poorly understood. This thesis focuses on deciphering the molecular mechanisms whereby the synthetic triterpenoids affect cell migration. I observed that the imidazolide and methyl ester derivatives of the synthetic triterpenoid, 2-cyano-3,12-dioxooleana-1,9-dien-28-oic aic acid …
Frazzled And Abelson Interact To Regulate The Actin Cytoskeleton In Drosophila, Bridget Elsa Varughese
Frazzled And Abelson Interact To Regulate The Actin Cytoskeleton In Drosophila, Bridget Elsa Varughese
Wayne State University Dissertations
Guidance receptors such as Frazzled affect cell shape and motility by directly or indirectly modulating the cytoskeleton. Fra is particularly needed for the formation of the posterior commissures in a developing Drosophila embryo. The cytoplasmic tyrosine kinase, Abelson Kinase (Abl) enhances the loss of commissures observed in fra mutant. Abl physically interacts with Frazzled to help guide commissural axons across the midline. Furthermore, the loss of commissural axons is only seen when the actin dynamics are perturbed. Abl is also known to regulate actin-dependent processes underlying formation of filopodia, microspikes and membrane ruffles. So, we established a Drosophila S2 cell …
Rho Kinase Regulates Basement Membrane Dynamics To Coordinate Salivary Gland Branching Morphogenesis And Tissue Organization, William Patrick Daley
Rho Kinase Regulates Basement Membrane Dynamics To Coordinate Salivary Gland Branching Morphogenesis And Tissue Organization, William Patrick Daley
Legacy Theses & Dissertations (2009 - 2024)
Branching morphogenesis is a developmental mechanism utilized by many organs, including the salivary gland, lung, kidney, and mammary gland, to increase the epithelial surface area for secretion or absorption. The embryonic submandibular salivary gland (SMG) is a classic model for studying tissue morphogenesis in three dimensions ex vivo. Salivary gland development requires distinct but overlapping processes: morphogenesis, the actual physical rearrangement of cells into complex three dimensional structures, and cytodifferentiation, the process by which these cells begin to take on their own specialized function. Polarization, or the organization of cells into a cohesive tissue structure, also occurs concurrently during organ …