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Articles 1 - 28 of 28
Full-Text Articles in Cell Biology
Terahertz Imaging Platform To Characterize The Growth Of In-Vitro Breast Tumors, Scarlett-Marie Acklin
Terahertz Imaging Platform To Characterize The Growth Of In-Vitro Breast Tumors, Scarlett-Marie Acklin
Inquiry: The University of Arkansas Undergraduate Research Journal
This study aimed at evaluating the ideal plating method and density for imaging with the terahertz (THz) spectrometer. In this study, different methods were used to grow in-vitro tumors using the 4T1 cell line. Here, attempts to grow breast tumors in-vitro were conducted. Results were produced in two environments, flat-bottomed plates and round-bottomed multiwell plates. The second method allowed for faster clumping and increased cell aggregation, producing tumors up to 7mm. Terahertz spectroscopy produced images that correlated well to photomicrographs taken of the in-vitro tumors. This methodology shows great promise for providing a reliable, parameter-controlled source of in-vitro breast tumors …
Evolution Of Gene Structure In Multicellular Eukaryotes, Maria Hester
Evolution Of Gene Structure In Multicellular Eukaryotes, Maria Hester
Inquiry: The University of Arkansas Undergraduate Research Journal
We investigated the patterns of intron conservation in eukaryotes for five different genes. The genes examined were ribosomal proteins L8, S14 and S17, along with elongation factor 2B and triose phosphate isomerase. Intron conservation for S14, S17, and triose phosphate isomerase was determined for 32 species representing the major branches of multicellular eukaryotes. For 25 conserved introns 16 were phase 0, five were phase 1, and four were phase 2. Triose phosphate isomerase had five of nine conserved introns shared between plants and animals, where S14 had one of nine and S17 had one of seven. However, there were two …
The Effects Of Nematode Infection And Mi-Mediated Resistance In Tomato (Solanum Lycopersicum) On Plant Fitness, Brandon P. Corbett
The Effects Of Nematode Infection And Mi-Mediated Resistance In Tomato (Solanum Lycopersicum) On Plant Fitness, Brandon P. Corbett
Inquiry: The University of Arkansas Undergraduate Research Journal
The Mi gene in tomato (Solanum lycopersicum) is a single, dominant resistance (R) gene that confers resistance against several species of insects and root-knot nematodes. Tis study examined the impact of root-knot nematode infestation and the plant growth and reproduction of near-isogenic tomato cultivars with and without Mi. The objectives of this experiment were to examine the potential fitness costs and benefits of the R gene-mediated herbivore resistance, and to explore the role of nematodes as a selection pressure favoring plants that carry Mi. Mi-mediated resistance dramatically reduced nematode reproduction on tomato. In the presence of nematodes, plants that carried …
Design Of A Bioreactor To Study The Role Of Red Blood Cells In The Transport Of Nitric Oxide In The Microcirculation, Nupura Bhise, Mahendra Kavdia
Design Of A Bioreactor To Study The Role Of Red Blood Cells In The Transport Of Nitric Oxide In The Microcirculation, Nupura Bhise, Mahendra Kavdia
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Nitric oxide (NO) plays an important role in physiological functions like vasodilation, neurotransmission, and inhibition of platelet aggregation. The endothelium-derived NO diffuses into the vascular lumen where it interacts with flowing blood as well as the smooth muscles where it modulates vascular tone. However, uncertainty exists on how NO escapes the rapid scavenging by hemoglobin (Hb) and reaches smooth muscles. Several proposed hypotheses include 1) a reduced reaction rate of NO with Hb contained inside red blood cells (RBCs) and 2) NO preservation in the bound form of s-nitrosohemoglobin or nitrite. The mechanism and magnitude of reduction of NO reaction …
Synthesis And Evaluation Of New Cathepsin D Inhibitors, Rose M. Mcconnell, Walter E. Godwin, Kelley Sayyer, Carol Trana, Adam Green, Matthew Mcconnell, Ashley Young, Lauren Young, Susan E. Hatfield
Synthesis And Evaluation Of New Cathepsin D Inhibitors, Rose M. Mcconnell, Walter E. Godwin, Kelley Sayyer, Carol Trana, Adam Green, Matthew Mcconnell, Ashley Young, Lauren Young, Susan E. Hatfield
Journal of the Arkansas Academy of Science
Cathepsin D, a lysosomal aspartic protease, has been suggested to play a role in the metastatic potential of several types of cancer A high activated cathepsin D level in breast tumor tissue has been associated with an increased incidence of relapse and metastasis. High levels of active cathepsin D have also been found in colon cancer, prostate cancer, uterine cancer, and ovarian cancer. Hydroxyethyl isosteres with cyclic tertiary amine have proven to be clinically useful as inhibitors of aspartyl proteases, such as cathepsin D and the HIV1 aspartyl protease. Also cathepsin D has recently been associated with the development of …
Effect Of Ionizing Radiation On Trichomonas Vaginalis: Increase In Red Pigmented Intracellular Bodies Associated With Irradiated Cells, James J. Daly Sr., Terryl L. Hostetler, Max L. Baker
Effect Of Ionizing Radiation On Trichomonas Vaginalis: Increase In Red Pigmented Intracellular Bodies Associated With Irradiated Cells, James J. Daly Sr., Terryl L. Hostetler, Max L. Baker
Journal of the Arkansas Academy of Science
No abstract provided.
Actin Dynamics Regulate Myosin Assembly In Muscle Cells, John Dylan Cook
Actin Dynamics Regulate Myosin Assembly In Muscle Cells, John Dylan Cook
Inquiry: The University of Arkansas Undergraduate Research Journal
During muscle cell (i.e. myocyte )development, organization of actin and myosin into the contractile unit (i.e. sarcomere) is required for proper muscle contraction. By disrupting the processes of muscle cell development, I am able to see which steps play important roles in proper maturation of myocytes. Elucidation of the key pathways in muscle development could lead to a better understanding of human cardiac hypertrophies and muscle myopathies. Since actin filament formation precedes myosin organization, I am using actin assembly inhibitors to determine if actin filaments are a necessary prerequisite for myosin organization. It is hypothesized that disruption of actin will …
Examination Of The Cell Wall Of Micrasterias Radiosa Var Radiosa (Conjugatophyceae) By Transmission And Scanning Electron Microscopy, Jennifer A. Akin, Richard L. Meyer
Examination Of The Cell Wall Of Micrasterias Radiosa Var Radiosa (Conjugatophyceae) By Transmission And Scanning Electron Microscopy, Jennifer A. Akin, Richard L. Meyer
Journal of the Arkansas Academy of Science
The cell wall of Micrasterias radiosa var. radiosa Ralfs 1848 (Conjugatophyceae) was examined by transmission and scanning electron microscopy. Previous electron microscopy of this taxon has not been performed; thus these are new observations. The cell wall was recognized to be of the Cosmarium type with complex pores external to the plasma membrane that penetrate the secondary wall and with ornamentation arising from the secondary wall. Subdivided regions of the pore apparatus, the pore head, pore bulb, connecting pore channel, and pore depression were detected. Pores of type 4 were located in the isthmal region and at the division of …
Liver Lipids Profiles In Nude Mice Implanted Subcutaneously With Cells Of Human Prostate Adenocarcinoma Grade Iv, Lawrence M. Mwasi
Liver Lipids Profiles In Nude Mice Implanted Subcutaneously With Cells Of Human Prostate Adenocarcinoma Grade Iv, Lawrence M. Mwasi
Journal of the Arkansas Academy of Science
Liver lipid changes in male BALB-c nude mice due to subcutaneously implanted human prostate metastatic grade IV adenocarcinoma was studied. The prostate cancer cells were cultured in F12 plus 7.5% horse serum and 25% fetal calf serum medium. When they reached confluence, some of these cells were fixed with glutaraldehyde and thoroughly washed with buffer then 4 x107 cells were implanted into four mice. Four more mice were implanted with 4 x 106 viable, unfixed cells. Four uninjected mice served as controls. All the mice were sacrificed 18 days later. The total liver lipids (TLL) from each liver were extracted …
Correlation Between Chromatid Deletion Production And Progression Of The Dna Replication Fork In Uv-Irradiated S Phase Xenopus Cells, Daniel M. Yoder, Jason M. Hiles, H. Gaston Griggs
Correlation Between Chromatid Deletion Production And Progression Of The Dna Replication Fork In Uv-Irradiated S Phase Xenopus Cells, Daniel M. Yoder, Jason M. Hiles, H. Gaston Griggs
Journal of the Arkansas Academy of Science
Experimentation was performed primarily to determine whether progression of the DNA replication fork along segments of S phase Xenopus chromosomes, which contain UV-induced pre-aberrational lesions, plays a significant role in conversion of these lesions into chromatid deletions. Specifically, a Xenopus chromosome that was both easy to identify and that possessed a single DNA replication fork in one arm was found and used to conduct the experimentation. This chromosome was exposed to UV in early S phase and a Bromodeoxyuridine/Giemsa differential staining technique was applied in conjunction with conventional aberrational techniques to correlate progression of the DNA replication fork through segments …
Photoreactivation Of Uv-Induced Damage In G1 Phase Xenopus Cells That Leads To A Sister Chromatid Exchanged And Cell Death, Dawn Laswell, Jennifer Barber, H. Gaston Griggs
Photoreactivation Of Uv-Induced Damage In G1 Phase Xenopus Cells That Leads To A Sister Chromatid Exchanged And Cell Death, Dawn Laswell, Jennifer Barber, H. Gaston Griggs
Journal of the Arkansas Academy of Science
Experiments were conducted with the A87 Xenopus tissue culture cell line which centered on use of the line's efficient photoreactivation (PR) mechanism to: (1) determine the extent to which sister chromatid exchanges (SCEs), induced by exposing early G1 phase cells to low UV fluenced, are photoreactivable, and (2) determine the extent to which the photoreactivable SCEs resulting from these low UV fluences constitute lethal lesions. For the first determination, UV fluences - SCE frequency relations and UV fluence + PR fluence - SCE frequency relations were established for UV fluences in the range 0-12 J/m2 and a single PR fluence …
Time Course Of Photoreactivation Of Uv Induced Damage In G1 Phase Xenopus Cells That Leads To Chromosome Breaks Observable By Premature Chromosome Condensation, Robert Wright, Stephen Ruble, H. Gaston Griggs
Time Course Of Photoreactivation Of Uv Induced Damage In G1 Phase Xenopus Cells That Leads To Chromosome Breaks Observable By Premature Chromosome Condensation, Robert Wright, Stephen Ruble, H. Gaston Griggs
Journal of the Arkansas Academy of Science
No abstract provided.
Photoreactivation Of The Effect Of Uv Light On Gamma Ray Induced Chromosome Aberration Production In G1 Phase Xenophus Cells, Rebecca Rowe, H. Gaston Griggs
Photoreactivation Of The Effect Of Uv Light On Gamma Ray Induced Chromosome Aberration Production In G1 Phase Xenophus Cells, Rebecca Rowe, H. Gaston Griggs
Journal of the Arkansas Academy of Science
No abstract provided.
Photoreactivation Of Chromatid Deletions Induced By Uv-Irradiation Of G1 Phase Hamster X Xenophus Hybrid Cells, Joel Staggers, H. Gaston Griggs
Photoreactivation Of Chromatid Deletions Induced By Uv-Irradiation Of G1 Phase Hamster X Xenophus Hybrid Cells, Joel Staggers, H. Gaston Griggs
Journal of the Arkansas Academy of Science
No abstract provided.
Repair Of Ultraviolet And Gamma-Ray Induced Lethal Damage In An Insect Tissue Culture Cell Line, Craig Endres, Susan Kulp, H. Gaston Griggs
Repair Of Ultraviolet And Gamma-Ray Induced Lethal Damage In An Insect Tissue Culture Cell Line, Craig Endres, Susan Kulp, H. Gaston Griggs
Journal of the Arkansas Academy of Science
No abstract provided.
Dark Repair Of Lethal Damage Induced In A Hybrid Mammalian Tissue Culture Cell Line By Ultraviolet Light, Robin Haetten, Margaret Mcguinness, H. Gaston Griggs
Dark Repair Of Lethal Damage Induced In A Hybrid Mammalian Tissue Culture Cell Line By Ultraviolet Light, Robin Haetten, Margaret Mcguinness, H. Gaston Griggs
Journal of the Arkansas Academy of Science
No abstract provided.
Time Course Of Pr Of Uv-Induced Chromosomal Aberrations And Lethal Damage In S And G2 Xenopus Cells, Jan Payne, H. Gaston Griggs
Time Course Of Pr Of Uv-Induced Chromosomal Aberrations And Lethal Damage In S And G2 Xenopus Cells, Jan Payne, H. Gaston Griggs
Journal of the Arkansas Academy of Science
Sand G2 phase cells were exposed to 150 ergs mm⁻² UV and their ability to photoreactivate the induced cell killing (loss of colony forming ability) and chromosomal aberrations was determined as a function of time following the UV exposure. In S phase cells, the lesions leading to cell death and those leading to aberrations were both converted to a non-photoreactivable state shortly after the UV exposure. A significant fraction of the lesions induced in G2 cells, that led to cell death, were converted to a non-photoreactivable state before the progeny of the exposed cells reached the next succeeding S phase. …
Scanning Electron Microscope Study Of Brachysclereids Of Pear (Pyrus Communis L.), Clarence B. Sinclair
Scanning Electron Microscope Study Of Brachysclereids Of Pear (Pyrus Communis L.), Clarence B. Sinclair
Journal of the Arkansas Academy of Science
The external surfaces of pear sclereids commonly are illustrated as covered with apertures. This SEAA investigation of the surface features has shown the surface to have few or no apertures. When the primary wall layer was removed the typical ramiform canal system was obvious. This observation confirms the often-ignored fact that the pitapertures of the secondary wall are not continuous with the primary wall. Hence, they do not show on the surfaces of the intact cell.
Light And Electron Microscope Study Of The Mitotic Apparatus Of The Ring-Legged Earwig, Euborellia Annulipes (Lucas), Vonnie R. Prentice, William L. Evans
Light And Electron Microscope Study Of The Mitotic Apparatus Of The Ring-Legged Earwig, Euborellia Annulipes (Lucas), Vonnie R. Prentice, William L. Evans
Journal of the Arkansas Academy of Science
The mitotic apparatus in dividing cells of the ring-legged earwig is composed of the cell center or pole, chromosomal fibers, continuous fibers, and chromosomal and background structure. Parts from cellular elements such as Golgi membranes, endoplasmic reticulum, and nuclear envelope as well as whole mitochondria associate with the spindle microtubules to produce the diffraction pattern of the spindle fibers as seen with the light microscope. The microtubules of the chromosomal fibers attach independently and demonstrate the diffuse nature of the centromere or polycentric condition of the chromosomes.
Rapid Electroosmosis Measurements, James O. Wear
Rapid Electroosmosis Measurements, James O. Wear
Journal of the Arkansas Academy of Science
A cell has been designed and built that allows for rapid measurement of volume moved in a definite time by electroosmosis. The cell is simple to use and is not very elaborate. Using a water jacket, the cell temperature can be controlled to ± 0.1° C. Measurements are presented for acetonitrile, dimethylformamide, and nitrobenzene at 25° C for applied voltages of 25, 50, 75, and 100 volts.
Effects Of Urethan On Fish Epithelial And Fibroblast Cells In Vitro, Leland F. Morgans
Effects Of Urethan On Fish Epithelial And Fibroblast Cells In Vitro, Leland F. Morgans
Journal of the Arkansas Academy of Science
The effects of urethan on RTG-2 and FHM cells were studied in vitro, by using the mitotic index, it was determined that 0.3 percent urethan caused an increase in the rate of cell division while higher concentrations (0.6, 0.9, 1.2, and 1.5 percent) caused either a decrease in the rate or a cessation of cell division. Concentrations of urethan higher than 1.5 percent killed the cells. The mitotic index data also indicated that epithelial cells continued to divide at a higher concentration of urethan than did the fibroblast cells. The morphological effects of urethan on the two cell lines were …
Hemolysis By Crotalus Horridus Atricaudatus Venom, Franklin E. Byrd, Bob D. Johnson
Hemolysis By Crotalus Horridus Atricaudatus Venom, Franklin E. Byrd, Bob D. Johnson
Journal of the Arkansas Academy of Science
No abstract provided.
Microirradiation Of Living Fungal Cells With A Laser, John L. Robertson, Charles L. Wilson
Microirradiation Of Living Fungal Cells With A Laser, John L. Robertson, Charles L. Wilson
Journal of the Arkansas Academy of Science
No abstract provided.
Effects Of Hydroxyurea On Cultured Somatic Cells Of The Chinese Hamster, Cricetulus Griseus, Charlotte Neill, William C. Guest
Effects Of Hydroxyurea On Cultured Somatic Cells Of The Chinese Hamster, Cricetulus Griseus, Charlotte Neill, William C. Guest
Journal of the Arkansas Academy of Science
No abstract provided.
Proposed Mechanisms Of Asexual Nuclear Division In Neurospora Crassa Shear And Dodge, Charles L. Wilson, John A. Brushaber, James R. Aist
Proposed Mechanisms Of Asexual Nuclear Division In Neurospora Crassa Shear And Dodge, Charles L. Wilson, John A. Brushaber, James R. Aist
Journal of the Arkansas Academy of Science
No abstract provided.
Observations On Nuclear Division In Vegetative Hyphae Of Ceratocystis Fagacearum, James R. Aist, Charles L. Wilson
Observations On Nuclear Division In Vegetative Hyphae Of Ceratocystis Fagacearum, James R. Aist, Charles L. Wilson
Journal of the Arkansas Academy of Science
No abstract provided.
Some Effects Of Ionizing Radiation On The Oral Suckers Of Rana Pipiens Embryos, Charles C. Reed
Some Effects Of Ionizing Radiation On The Oral Suckers Of Rana Pipiens Embryos, Charles C. Reed
Journal of the Arkansas Academy of Science
No abstract provided.
Epidermal Pore Of Oxcymitra Paleacea Bischoff, Eugene B. Wittlake
Epidermal Pore Of Oxcymitra Paleacea Bischoff, Eugene B. Wittlake
Journal of the Arkansas Academy of Science
No abstract provided.