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George McNamara

2014

GFP T-cells

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Image Z-Series Wrt Nvidia Titan 6 Gb Gpu Cuda Deconvolution, George Mcnamara Apr 2014

Image Z-Series Wrt Nvidia Titan 6 Gb Gpu Cuda Deconvolution, George Mcnamara

George McNamara

Image Z-series wrt NVidia Titan 6 Gb GPU CUDA Deconvolution

This ZIP file contains raw and deconvolved data series from George McNamara, Laurence J.N. Cooper lab, M.D. Anderson Cancer Center. I have also included our lab instructions, and a screenshot of the current CUDA deconvolution settings (for dry objective lenses, Manish Butte wrote me to use the immersion medium refractive index, air = 1.0).

Instrument:

Leica DMI6000 inverted fluorescence microscope, Lumencor SOLA LED light source, Leica "L5" GFP filter cube (480/40ex, 505dm, 527/30em).

Leica 20x/0.75 NA objective lens, 1.6x optovar, for 325 nm XY piel size. For this dataset, I …