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Full-Text Articles in Cell Biology
Endothelin-1 Induced Phosphorylation Of Erk1/2 In Bovine Corneal Endothelial Cells, Akhila Bethi
Endothelin-1 Induced Phosphorylation Of Erk1/2 In Bovine Corneal Endothelial Cells, Akhila Bethi
Masters Theses & Specialist Projects
The purpose of this study was to determine whether Endothelin-1 (ET-1) induced cellular responses in bovine corneal endothelial cells (BCECs) involves MAPK pathway by phosphorylating ERK1/2 protein kinase and to find out the phosphorylation patterns of ERK1/2 in confluent and sub-confluent cells. BCECs were isolated from bovine corneas and cultured in medium supplemented with 10% serum. Confluent (contact inhibited) and sub-confluent (actively growing cells) serum starved cells grown in T-75 flasks were treated with 10nM Endothelin-1. The control cells were left untreated. Total cellular protein was isolated using RIPA buffer and quantified according to the Peterson modification of the Lowry …
Next Generation Sequencing Reveals Gene Expression Patterns In The Zebrafish Inner Ear Following Growth Hormone Injection, Gopinath Rajadinakaran
Next Generation Sequencing Reveals Gene Expression Patterns In The Zebrafish Inner Ear Following Growth Hormone Injection, Gopinath Rajadinakaran
Masters Theses & Specialist Projects
Loss of hair cells due to acoustic trauma results in the loss of hearing. In humans, unlike other vertebrates, the mechanism of hair cell regeneration is not possible. The molecular mechanisms that underlie this regeneration in nonmammalian vertebrates remain elusive. To understand the gene regulation during hair cell regeneration, our previous microarray study on zebrafish inner ears found that growth hormone (GH) was significantly upregulated after noise exposure. In this current study, we utilized Next Generation Sequencing (NGS) to examine the genes and pathways that are significantly regulated in the zebrafish inner ear following sound exposure and GH injection. Four …
Plant-Like Cryptochrome Does Not Promote Blue Light-Induced Resetting Of The Circadian Clock In Chlamydomonas Reinhardtii, Jonathan Howton
Plant-Like Cryptochrome Does Not Promote Blue Light-Induced Resetting Of The Circadian Clock In Chlamydomonas Reinhardtii, Jonathan Howton
Masters Theses & Specialist Projects
The circadian clock is an endogenous timer that allows an organism to anticipate and properly prepare for the daily changes in the environment. This preparation occurs in the form of daily rhythms in metabolism, physiology, and behavior. These approximately 24-hour rhythms are reset upon environmental time cues such as the daily light/dark and temperature cycles. The unicellular green alga Chlamydomonas reinhardtii is a useful model organism for circadian clock research. It shows several well-characterized circadian rhythms of behavior, and the monitoring of its rhythm of phototaxis, or swimming towards light, has been automated. The receptors involved in entraining the clock …