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Articles 1 - 8 of 8
Full-Text Articles in Cell Biology
Regulation Of Er To Golgi Transport By Alg-2 And Peflin In Response To Calcium Signaling, And A Review Of Relevant Genetically-Encoded Fluorescent Tools, Danette M. Kowal Seiler
Regulation Of Er To Golgi Transport By Alg-2 And Peflin In Response To Calcium Signaling, And A Review Of Relevant Genetically-Encoded Fluorescent Tools, Danette M. Kowal Seiler
Graduate Student Theses, Dissertations, & Professional Papers
The endoplasmic reticulum performs multiple functions in the eurkaryotic cell: biomolecule synthesis, protein folding, cargo sorting and secretion, and helping to maintain cellular calcium homeostasis. Though much has been discovered about these processes and the myriad proteins, enzymes, transcription factors, ions and other factors that play their own parts, much is still left to learn. To facilitate continued study of ER-to-Golgi transport, an array of genetically-encoded fluorescent tools are available to researchers. The featured review in Chapter 1 details the history of these tools and highlights some of the newest, which also incorporate information from the fields of synthetic chemistry …
Understanding Cellular Dynamics: Investigating A Yeast-Two Hybrid Protein-Protein Interaction, Riok3:Cse1l; Also By Investigating Trogocytosis Of Cd4+ T Cells From Rhog-/- Mice, Brittnee N. Crane
Graduate Student Theses, Dissertations, & Professional Papers
Rift Valley fever virus (RVFV) is a negative-sense virus that was first described in 1930 when it was isolated from sheep during an outbreak. In the last 25 years there has been a spread in the geographical range into the Middle East. The most susceptible animals are cattle, goats, sheep, and camels. The most overt symptom of livestock infection is that infected pregnant animals experience spontaneous abortion. Humans are susceptible as well and symptoms range from mild febrile illness to liver necrosis, blindness, hemorrhagic fever, and death. An important player in the cellular innate response to RVFV infections is the …
Investigating Changes In Er-To-Golgi Protein Transport Following A Short Calcium Pulse: Roles Of Alg-2 And Peflin, Alaa Mohammed Yosri Shaheen
Investigating Changes In Er-To-Golgi Protein Transport Following A Short Calcium Pulse: Roles Of Alg-2 And Peflin, Alaa Mohammed Yosri Shaheen
Graduate Student Theses, Dissertations, & Professional Papers
The ER-Golgi interface is a very dynamic environment that involves the movement of protein-loaded vesicles forward and backward. The movement of COPII-coated vesicles from the ER to the Golgi is the initial step in the movement of secreted, organellar, and cell surface proteins toward their final destinations. Many factors can regulate this step, including cytosolic calcium increases. In this study, we examined the effect of a transient calcium pulse on recruitment to ER exit sites of cargo proteins for ER export, the calcium-sensitive regulatory proteins apoptosis-linked gene 2 (ALG-2) and peflin, and the COPII outer coat subunits Sec31A and Sec13. …
Alternative Splicing Regulates The Innate Immune Response To Viral Infection, Luke A. White
Alternative Splicing Regulates The Innate Immune Response To Viral Infection, Luke A. White
Graduate Student Theses, Dissertations, & Professional Papers
Rift Valley fever virus (RVFV) is a mosquito-borne RNA virus that infects humans and livestock in sub-Saharan Africa and the Arabian peninsula, causing disease ranging from a mild flu-like illness to liver damage, blindness, hemorrhagic fever, death, and, especially in livestock animals, high rates of abortive pregnancies. There is no approved vaccine for RVFV, and as a disease with a high rate of spread that causes severe illness, it is listed as a Category A pathogen by the USA CDC. A better understanding of RVFV’s molecular virology will be instrumental to combating RVFV as climate change causes its mosquito host …
Cop9 Signalosome Component Csn-5 Promotes Accumulation And Function Of Stem Cell Regulators Fbf-1 And Fbf-2, Emily Osterli
Cop9 Signalosome Component Csn-5 Promotes Accumulation And Function Of Stem Cell Regulators Fbf-1 And Fbf-2, Emily Osterli
Graduate Student Theses, Dissertations, & Professional Papers
RNA-binding proteins FBF-1 and FBF-2 (FBFs) are required for stem cell maintenance in C. elegans, although the mechanisms by which FBFs protein levels are regulated remain unknown. Using a yeast two-hybrid screen, we identified an interaction between both FBFs and CSN-5, a component of the COP9 (constitutive photomorphogenesis 9) signalosome. This highly conserved COP9 complex can affect protein stability through a range of mechanisms including deneddylation, deubiquitination, and phosphorylation (Wolf et al., 2003). Mapping protein-protein interactions between FBFs and CSN-5 suggested that the MPN (Mpr1/Pad1 N-terminal) metalloprotease domain of CSN-5 interacts with the RNA-binding domain of FBFs at physiologically …
Characterizing The Requirements For The Matricellular Protein, Dccn, In Nervous System Function, Elizabeth L. Catudio Garrett
Characterizing The Requirements For The Matricellular Protein, Dccn, In Nervous System Function, Elizabeth L. Catudio Garrett
Graduate Student Theses, Dissertations, & Professional Papers
The brain is organized as a complex network of specialized neurons that communicate via a combination of electrical and chemical signals. Our brains function to generate movement, control organ function, or direct complex behaviors; all of which requires the ability to regulate the flow of communication between circuits and networks. Work in this thesis addresses two areas of neuron communication: first, how does the release of more than one neurotransmitter from a single neuron impact behavior, and second, are matricellular proteins (MCPs) key contributors to synaptic transmission and neuron function? The conserved CCN family of MCPs have a …
Dynein Light Chain 1 Functions As A Cofactor For Post-Transcriptional Mrna Regulation And Rna Granule Assembly, Nicholas Day
Dynein Light Chain 1 Functions As A Cofactor For Post-Transcriptional Mrna Regulation And Rna Granule Assembly, Nicholas Day
Graduate Student Theses, Dissertations, & Professional Papers
Gene regulation is essential for ensuring maintenance, proliferation, and proper development of a cell. RNA binding proteins (RBPs) regulate gene expression by targeting and binding mRNAs to control their translation and often localize to cytoplasmic assemblies of protein and RNA called RNA granules to facilitate post-transcriptional mRNA regulation. Using C. elegans as a model organism, we report on the function of dynein light chain 1 (DLC-1), a subunit of the dynein motor complex, in post-transcriptional mRNA regulation in the gonad. Previous work suggests that DLC-1 is an RBP cofactor that functions independent of the dynein motor. It is unknown how …
Characterization Of The Influences Of Human Cytomegalovirus Glycoprotein O (Go) Expression On Gh/Gl Complexes Assembly And Its Polymorphisms On Cell-Free And Cell-To-Cell Spread, And Antibody Neutralization., Le Z. Day
Graduate Student Theses, Dissertations, & Professional Papers
Human cytomegalovirus (HCMV) is widely spread throughout the world and immunocompromised individuals can suffer severe diseases from HCMV infection. Once the infection is established, HCMV can spread through the body and infect many major somatic cell types. The glycoproteins H and L (gH/gL) on HCMV envelope can be bound by either gO or the UL128-131 proteins to form complexes gH/gL/gO and gH/gL/UL128-131 that are critical for viral entry and spread, and these two complexes are important targets of neutralizing antibodies. Strains of HCMV vary considerably in the levels of gH/gL/gO and gH/gL/UL128-131. gO is one of the most diverse loci …