Cell Biology Commons^™

Expression And Characterization Of The Flocculin Flo11/Muc1, A Yeast Mannoprotein With Homotypic Properties Of Adhesion, Li Li Ph.D., Lois M. Douglas, Yang Yang, A M. Dranginis

Faculty Works: Biology, Chemistry, and Environmental Studies

The Flo11/Muc1 flocculin has diverse phenotypic effects. Saccharomyces cerevisiae cells of strain background Σ1278b require Flo11p to form pseudohyphae, invade agar, adhere to plastic, and develop biofilms, but they do not flocculate. We show that S. cerevisiae var. diastaticusstrains, on the other hand, exhibit Flo11-dependent flocculation and biofilm formation but do not invade agar or form pseudohyphae. In order to study the nature of the Flo11p proteins produced by these two types of strains, we examined secreted Flo11p, encoded by a plasmid-borne gene, in which the glycosylphosphatidylinositol anchor sequences had been replaced by a histidine tag. A protein of …