Cell Biology Commons^™

Examination Of Human Embryonic Kidney Cells And Cardiomyocytes Using Glass Microcarrier Beads And Scanning Electron Microscopy, Jaekook Sim

Undergraduate Research Symposium

Alterations of sarcomeric proteins lead to disruption of myofilaments and are associated with hypertrophic cardiomyopathy. We have identified a genetically altered mouse strain with an elevated level of actin associated protein and are characterizing the nature of the hypertrophy by examining the cultured cells on glass microcarrier beads using Scanning Electron Microscopy (SEM). Beads provide a surface for cell growth and division and subsequent analysis of myocyte morphology. This study requires the establishment of primary embryonic cardiomyocyte culture which is difficult to establish. Therefore in initial studies to acquire the necessary tissue culture skill, we cultured Human Embryonic Kidney (HEK) …