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In Situ Regulation Of Cytosolic Phospolipase A₂, Beverly A. Rzigalinski
In Situ Regulation Of Cytosolic Phospolipase A₂, Beverly A. Rzigalinski
Theses and Dissertations in Biomedical Sciences
The 85 kDa cytosolic phospholipase A2 (cPLA2) is an agonist-responsive effector for intracellular signal transduction through the arachidonate cascade. In vitro studies have demonstrated that this enzyme is regulated by sub-micromolar calcium and is specific for arachidonate as the sn-2 fatty acyl group of phospholipid substrates. However, very little data is available regarding in situ mechanisms which govern the activity of cPLA2. The primarily objective of these studies was to develop an in situ system for the study of cPLA2, and investigate mobilization of arachidonate during signal transduction events.
Dimethylsulfoxide differentiation of the …