Open Access. Powered by Scholars. Published by Universities.®
Cell and Developmental Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Institution
-
- University of Kentucky (5)
- City University of New York (CUNY) (4)
- Washington University in St. Louis (4)
- University of Dayton (3)
- Western University (3)
-
- Selected Works (2)
- Aga Khan University (1)
- Claremont Colleges (1)
- Florida International University (1)
- James Madison University (1)
- Rollins College (1)
- Touro College and University System (1)
- University of Connecticut (1)
- University of Louisville (1)
- University of Lynchburg (1)
- University of Massachusetts Amherst (1)
- University of Nebraska Medical Center (1)
- University of Puget Sound (1)
- University of Wisconsin Milwaukee (1)
- University of the Pacific (1)
- Virginia Commonwealth University (1)
- Keyword
-
- Cancer (2)
- Drosophila melanogaster (2)
- Epigenetics (2)
- Eye (2)
- Gene expression (2)
-
- Genetics (2)
- Symbiosis (2)
- ATG-9 (1)
- Acute myocardial infarction (1)
- Adhesion GPCRs (1)
- Adult stem cells (1)
- Aggregation (1)
- Ambystoma mexicanum (1)
- Amyotrophic lateral sclerosis (1)
- Animals (1)
- Arabidopsis thaliana (1)
- Atrial fibrillation (1)
- Autophagosome (1)
- Autophagy (1)
- Axolotl (1)
- BMP (1)
- Biofilms (1)
- C. ELEGANS (1)
- CRISPR (1)
- CUTICLE COLLAGENS (1)
- Cell Proliferation (1)
- Cells (1)
- Cellular behavior (1)
- Chaperone (1)
- Co-extraction (1)
- Publication
-
- Arts & Sciences Electronic Theses and Dissertations (4)
- Biology Faculty Publications (4)
- Dissertations, Theses, and Capstone Projects (3)
- Electronic Thesis and Dissertation Repository (3)
- Theses and Dissertations (2)
-
- Andrew C. Hillier (1)
- CMC Senior Theses (1)
- Department of Biological & Biomedical Sciences (1)
- Doctoral Dissertations (1)
- Electronic Theses and Dissertations (1)
- FIU Electronic Theses and Dissertations (1)
- Faculty Publications (1)
- Markey Cancer Center Faculty Publications (1)
- Molecular Modeling and Biopharmaceutical Center Faculty Publications (1)
- Molecular and Cellular Biochemistry Faculty Publications (1)
- NYMC Faculty Posters (1)
- Robert J. Wilkens (1)
- Science Seminar Series (1)
- Senior Honors Projects, 2010-2019 (1)
- Student Scholar Showcase (1)
- Student Theses (1)
- Summer Research (1)
- Theses & Dissertations (1)
- Theses and Dissertations--Biology (1)
- University Scholar Projects (1)
- Publication Type
- File Type
Articles 31 - 36 of 36
Full-Text Articles in Cell and Developmental Biology
Investigating The Essential Roles Of Dprl-1 In Drosophila Melanogaster, Alex Lee
Investigating The Essential Roles Of Dprl-1 In Drosophila Melanogaster, Alex Lee
Summer Research
Phosphatase of Regenerating Liver (PRL) proteins regulate a number of important cellular processes, including cell growth and division. Humans have three PRL proteins: PRL-1, PRL-2, and PRL-3. An accumulation of evidence has shown that elevated levels of PRLs are strongly correlated with uncontrollable growth and metastasis of tumors. However, contradictory findings have arisen indicating that PRLs instead function to halt cell division thereby preventing uncontrollable tumor growth. In light of these results, the underlying mechanisms regarding how PRLs function within cellular processes remains unclear. To investigate the functions of PRLs, we will create transgenic fruit flies (Drosophila melanogaster) …
Real-Time Quantitative Pcr To Demonstrate Gene Expression In An Undergraduate Lab, Abijeet Singh Mehta, Amit Singh
Real-Time Quantitative Pcr To Demonstrate Gene Expression In An Undergraduate Lab, Abijeet Singh Mehta, Amit Singh
Biology Faculty Publications
The objective of this teaching note is to develop a laboratory exercise, which allows students to get a hands-on experience of a molecular biology technique to analyze gene expression. The short duration of the biology laboratory for an undergraduate curriculum is the biggest challenge with the development of new labs. An important part of cell biology or molecular biology undergraduate curriculum is to study gene expression. There are many labs to study gene expression in qualitative manner. The commonly used reporter gene expression studies are primarily qualitative. However, there is no hands-on experience exercise to quantitatively determine gene expression. Therefore, …
An Undergraduate Cell Biology Lab: Western Blotting To Detect Proteins From Drosophila Eye, Neha Gogia, Ankita Sarkar, Amit Singh
An Undergraduate Cell Biology Lab: Western Blotting To Detect Proteins From Drosophila Eye, Neha Gogia, Ankita Sarkar, Amit Singh
Biology Faculty Publications
We have developed an undergraduate laboratory to allow detection and localization of proteins in the compound eye of Drosophila melanogaster, a.k.a fruit fly. This lab was a part of the undergraduate curriculum of the cell biology laboratory course aimed to demonstrate the use of Western Blotting technique to study protein localization in the adult eye of Drosophila. Western blotting, a two-day laboratory exercise, can be used to detect the presence of proteins of interests from total protein isolated from a tissue. The first day involves isolation of proteins from the tissue and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide) gel …
The Role Of The Dosage Compensation Complex As A Pathway For Spiroplasma To Induce Male Lethality In Drosophila Melanogaster, Becky Cheng
CMC Senior Theses
Drosophila melanogaster and many other insects harbor intracellular bacterial symbionts that are transmitted vertically from infected host mothers to their offspring. Many of these bacteria alter host reproductive developmental processes in order to increase their transmission success. For example, Spiroplasma, a spirochete that naturally infects D. melanogaster, selectively kills males during mid-embryogenesis while sparing females. Previous studies suggested that Spiroplasma interacts genetically with the male-specific dosage compensation pathway, which causes ~2-fold up-regulation of most genes located on the male’s single X chromosome so that their expression matches the levels found in females who have two Xs. To further …
Genetic Analysis Of Serf Gene Function In Drosophila Melanogaster And Its Contribution To A Fly Model Of Spinal Muscular Atrophy, Swagata Ghosh
Genetic Analysis Of Serf Gene Function In Drosophila Melanogaster And Its Contribution To A Fly Model Of Spinal Muscular Atrophy, Swagata Ghosh
Theses and Dissertations--Biology
The Serf gene is evolutionarily highly conserved but its biological function is not known in any organism. In human, SERF1/H4F5 was first identified as a modifier of the disease Spinal Muscular Atrophy (SMA). SMA is caused by mutations in the Survival Motor Neuron 1(SMN1) gene leading to diminished levels of the Smn protein. More than 90% of patients with the most severe form of SMA have deletions that remove SERF1 as well as mutaions within SMN1. Hence, loss of Serf activity is hypothesized to exacerbate SMA disease progression. The primary motivation of this thesis was to test …
The Role Of Klf1 In Regulating Γ-Globin Gene Repressors, Anna P. Kovilakath
The Role Of Klf1 In Regulating Γ-Globin Gene Repressors, Anna P. Kovilakath
Theses and Dissertations
Sickle cell disease and β-thalassemia affect millions of people worldwide. γ-globin is the fetal counterpart to the adult β-globin. Research has shown that affected patients with higher than normal γ-globin show less severe symptoms. Therefore, reversing or preventing the hemoglobin switch from γ- to β- globin is a promising avenue of research for treating these diseases. KLF1 is an erythroid transcription factor involved in hemoglobin switching. Herein, we show that KLF1 directly regulates the γ-globin repressor gene LRF in both the mouse and human systems. KLF1 may also directly activate γ-globin expression by binding the promoter. In human HUDEP-2 cells, …