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Controlling Pseudomonas Aeruginosa Biofilms And Persister Cells By Manipulating Cell-Cell Signaling, Shuyuan Ma

Theses - ALL

Bacterial cells often form sessile biofilms that are up to 1,000 times more resistant to antimicrobial agents than free-living cells. Meanwhile, bacteria produce a small subpopulation of slow-growing or non-growing persister cells that exhibit high tolerance to antibiotics. Both biofilms and persister cells play important roles in the recalcitrance of chronic infections. Recent studies have shown that bacterial cell-to-cell communication, named quorum sensing (QS), is involved in the biofilm and persister formation.

In this study, we investigated the effects of quorum sensing signals N-(3-oxododecanoyl)-homoserine lactone (3-oxo-C12-HSL) and N-butyryl homoserine lactone (C4-HSL) on isolated Pseudomonas aeruginosa PAO1 and PDO300 persister cells. …