Biotechnology Commons^™

Recurrent Modification Of A Conserved Cis-Regulatory Element Underlies Fruit Fly Pigmentation Diversity, William A. Rogers, Joseph R. Salomone, David J. Tacy, Eric M. Camino, Kristen A. Davis, Mark Rebeiz, Thomas M. Williams

Thomas M. Williams

The development of morphological traits occurs through the collective action of networks of genes connected at the level of gene expression. As any node in a network may be a target of evolutionary change, the recurrent targeting of the same node would indicate that the path of evolution is biased for the relevant trait and network. Although examples of parallel evolution have implicated recurrent modification of the same gene and cis-regulatory element (CRE), little is known about the mutational and molecular paths of parallel CRE evolution. InDrosophila melanogaster fruit flies, the Bric-à-brac (Bab) transcription factors control the development …

Protein Trap Lines Of Drosophila To Demonstrate Spatio-Temporal Localization Of Proteins In An Undergraduate Lab, Oorvashi Roy Puli, Amit Singh

Amit Singh

The objective of this teaching note is to generate a laboratory exercise, which allows students to get a hands-on experience of a cell biology technique. The short duration of the laboratory classes is the biggest challenge with the development of a cell biology lab for an undergraduate curriculum. Therefore, it is necessary to design a laboratory exercise that enables the students to carry out cell biological assays in the desired time. This laboratory exercise focuses on tracking protein expression levels along a spatial (space) and temporal (time) axis in developing Drosophila melanogaster organ primordium. Here we use the protein trap …

A Cell Biology Laboratory Exercise To Study Sub-Cellular Organelles In Drosophila, Meghana Tare, Amit Singh

Amit Singh

The fast-changing scenario of undergraduate education puts emphasis on introducing students to hands-on techniques as part of their laboratory courses. In order to cater to large numbers of students and the time constraints involved with undergraduate level laboratory courses, there is a need for development of experiments that are cost effective and can be completed in a defined time frame. We have devised a laboratory exercise for teaching cell biology using the Drosophila melanogaster model. Drosophila can be reared in a short period of time in a cost effective manner. We used Drosophila tissue to study the sub-cellular organization of …

Drosophila Adult Eye Model To Teach Scanning Electron Microscopy In An Undergraduate Cell Biology Laboratory, Meghana Tare, Oorvashi Roy Puli, Sarah M. Oros, Amit Singh

Amit Singh

We have devised an undergraduate laboratory exercise to study tissue morphology using fruit fly, Drosophila melanogaster, as the model organism. Drosophila can be reared in a cost effective manner in a short period of time. This experiment was a part of the undergraduate curriculum of the cell biology laboratory course aimed to demonstrate the use of scanning electron microscopy (SEM) technique to study the morphology of adult eye of Drosophila. The adult eye of Drosophila is a compound eye, which comprises of 800 unit eyes, and serves as an excellent model for SEM studies. We used flies that …

Genetic Changes To A Transcriptional Silencer Element Confers Phenotypic Diversity Within And Between Drosophila Species, Winslow C. Johnson, Alison J. Ordway, Masayoshi Watada, Jonathan N. Pruitt, Thomas M. Williams, Mark Rebeiz

Thomas M. Williams

The modification of transcriptional regulation has become increasingly appreciated as a major contributor to morphological evolution. However, the role of negative-acting control elements (e.g. silencers) in generating morphological diversity has been generally overlooked relative to positive-acting “enhancer” elements. The highly variable body coloration patterns among Drosophilid insects represents a powerful model system in which the molecular alterations that underlie phenotypic diversity can be defined. In a survey of pigment phenotypes among geographically disparate Japanese populations of Drosophila auraria, we discovered a remarkable degree of variation in male-specific abdominal coloration. In testing the expression patterns of the major pigment-producing enzymes, …

Design Of A Factorial Experiment With Randomization Restrictions To Assess Medical Device Performance On Vascular Tissue, Wiebke Diestelkamp, Carissa M. Krane, Margaret Pinnell

Carissa M. Krane

Background: Energy-based surgical scalpels are designed to efficiently transect and seal blood vessels using thermal energy to promote protein denaturation and coagulation. Assessment and design improvement of ultrasonic scalpel performance relies on both in vivo and ex vivo testing. The objective of this work was to design and implement a robust, experimental test matrix with randomization restrictions and predictive statistical power, which allowed for identification of those experimental variables that may affect the quality of the seal obtained ex vivo. Methods: The design of the experiment included three factors: temperature (two levels); the type of solution used to perfuse the …

Distinct Cardiac Responses To Heat Stress Between Two Broiler Lines Identified By Transcriptome Analysis, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont

Jibin Zhang

Mapping The Patent Landscape Of Synthetic Biology For Fine Chemical Production Pathways, Pablo Carbonell, Abdullah Gok, Philip Shapira, Jean-Loup Faulon

Philip Shapira

Effect Of Sugars On Artemisinin Production In Artemisia Annua L.: Transcription And Metabolite Measurements, Patrick Arsenault, Daniel Vail, Kristin Wobbe, Pamela Weathers

Kristin K. Wobbe

The biosynthesis of the valuable sesquiterpene anti-malarial, artemisinin, is known to respond to exogenous sugar concentrations. Here young Artemisia annua L. seedlings (strain YU) were used to measure the transcripts of six key genes in artemisinin biosynthesis in response to growth on sucrose, glucose, or fructose. The measured genes are: from the cytosolic arm of terpene biosynthesis, 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), farnesyl disphosphate (FPS); from the plastid arm of terpene biosynthesis, 1-deoxyxylulose-5-phosphate synthase (DXS), 1-deoxyxylulouse 5-phosphate reductoisomerase (DXR); from the dedicated artemisinin pathway amorpha-4,11-diene synthase (ADS), and the P450, CYP71AV1 (CYP). Changes in intracellular concentrations of artemisinin (AN) and its precursors, …

U.S. Billion-Ton Update: Biomass Supply For A Bioenergy And Bioproducts Industry, Robert D. Perlack, Laurence M. Eaton, Anthony F. Turhollow Jr., Matt H. Langholtz, Craig C. Brandt, Mark E. Downing, Robin L. Graham, Lynn L. Wright, Jacob M. Kavkewitz, Anna M. Shamey, Richard G. Nelson, Bryce J. Stokes, William L. Rooney, David J. Muth Jr., J. Richard Hess, Jared M. Abodeely, Chad Hellwinckel, Danial De La Torre Ugarte, Daniel C. Yoder, James P. Lyon, Timothy G. Rials, Timothy A. Volk, Thomas S. Buchholz, Lawrence P. Abrahamson, Robert P. Anex, Thomas B. Voigt, William Berguson, Don E. Riemenschneider, Douglas Karlen, Jane M. F. Johnson, Robert B. Mitchell, Kenneth P. Vogel, Edward P. Richard Jr., John Tatarko, Larry E. Wagner, Kenneth E. Skog, Patricia K. Lebow, Dennis P. Dykstra, Marilyn A. Buford, Patrick D. Miles, D. Andrew Scott, James H. Perdue, Robert B. Rummer, Jamie Barbour, John A. Stanturf, David B. Mckeever, Ronald S. Zalesny Jr., Edmund A. Gee, P. Daniel Cassidy, David Lightle

Douglas L Karlen

The Report, Biomass as Feedstock for a Bioenergy and Bioproducts Industry: The Technical Feasibility of a Billion-Ton Annual Supply (generally referred to as the Billion-Ton Study or 2005 BTS), was an estimate of “potential” biomass within the contiguous United States based on numerous assumptions about current and future inventory and production capacity, availability, and technology. In the 2005 BTS, a strategic analysis was undertaken to determine if U.S. agriculture and forest resources have the capability to potentially produce at least one billion dry tons of biomass annually, in a sustainable manner—enough to displace approximately 30% of the country’s present petroleum …

Early Seed Development Responses In Cereals Under Environmental Stresses, Kevin Begcy

Kevin Begcy

Plants are influenced by a large number of environmental factors. The search for higher yields at lower costs requires detailed knowledge of physiology, biochemistry, molecular and genetic level of plants, in order to optimize the relationship between different factors of production for maximum performance. Reproductive development has been demonstrated to be especially highly sensitive to environmental stresses. Within environmental stresses, drought and heat stress are the major restrictors of food production worldwide. With decreasing supplies of freshwater, increase in average temperature and predictions for the increased frequency of extreme precipitation events, there is a critical need for developing crops that …

Physical, Thermal, And Spectroscopic Characterization Of Biofield Energy Treated Potato Micropropagation Medium, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

Mahendra Kumar Trivedi

Physicochemical And Spectroscopic Characterization Of Biofield Energy Treated Gerbera Multiplication Medium, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

Mahendra Kumar Trivedi

Physicochemical Characterization Of Biofield Treated Orchid Maintenance/Replate Medium, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

Mahendra Kumar Trivedi

Mcnamara 201412 Nih Scap Innocentive Challenge Solution - T-Bow Rainbow T-Cells And Tumor Cells Spatial Multiplexing Gene Expression Reporter System – Plus Supplement Plus Posters - 20151027 - Please Download "75" Instead, George Mcnamara

George McNamara

McNamara 201412 NIH SCAP InnoCentive Challenge Solution - T-Bow Rainbow T-cells and Tumor Cells Spatial Multiplexing Gene Expression Reporter System – plus supplement plus posters - 20151027.

///

Please download the current 20151027 (October 27, 2015) Tattletales and T-Bow update from

http://works.bepress.com/gmcnamara/75/

The bepress web site is not letting me replace the old pdf here at "65" with the additional 10 pages update.

///

The download is my/Cooper lab solution (submission) to the 2014 NIH Single Cell Analysis Program (SCAP) InnoCentive Challenge, "Follow That Cell". I submitted the Solution on 20141215Mon (with 20 minutes to spare). The Challenge web page …

Tattletales And T-Bow Update 20151027tue, George Mcnamara

George McNamara

20151027Tue this "75"

http://works.bepress.com/gmcnamara/75

is my update of "65" posting

See text at

http://works.bepress.com/gmcnamara/65/

for text summary. The PDf here in "75" supersedes "65".

The PDF here has 10 pages added to the end from the "65" version (pages 40-49 of PDF when including the bepress cover page)..

here is the text in my cover page (bepress may add its own cover):

20151027Tue: added 10 page e-poster at bottom explaining Binary Tattletales and T-Bow. That is, binary with respect to protein components. For one color (number of repeats, epitope tags, FPs are examples, here rounded to convenient numbers):

1. 100 …

Antibiogram, Biochemical Reactions And Genotyping Characterization Of Biofield Treated Staphylococcus Aureus, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

Mahendra Kumar Trivedi

Evaluation Of Effective Operational Cycle Time And Bioprocess Parameters In A Sequential Batch Reactor For Efficient Organic And Nutrient Removal From Domestic Sewage, Sandip S. Magdum, Siva Kumar Varigala, Yogesh N. Patil, Gauri P. Minde, Janardhan B. Bornare, V. Kalyanraman

Sandip S. Magdum

Anaerobic, anoxic and aerobic environment can be simulated in the single stage sequential batch reactor (SBR) . Various design conditions viz., combinations of different phase time and different cycle time, hydraulic residence time (HRT), substrate loading rate, sludge age (SRT) and aeration time were analyzed for optimum biological treatment. The pilot runs were evaluated with the design conditions of food/microbe ratio (F/M) 0.2 per day and MLSS of 4950 mg/L. For the given design conditions 4, 5 and 6 hours cycle lengths were analyzed for their efficient performance with 30-33% of decant volume for sewage containing C:N:P of 100:8:2. Studies …

Evaluation Of Phenotyping And Genotyping Characteristic Of Shigella Sonnei After Biofield Treatment, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana

Mahendra Kumar Trivedi

Effect Of Biofield Treatment On Phenotypic And Genotypic Characteristic Of Provindencia Rettgeri, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana

Mahendra Kumar Trivedi

Potential Impact Of Biofield Treatment On Atomic And Physical Characteristics Of Magnesium, Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Omprakash Latiyal, Snehasis Jana

Mahendra Kumar Trivedi

Biofield Treatment: A Potential Strategy For Modification Of Physical And Thermal Properties Of Indole, Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh K. Mishra, Snehasis Jana

Mahendra Kumar Trivedi

Impact Of Biofield Treatment On Atomic And Structural Characteristics Of Barium Titanate Powder, Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana

Mahendra Kumar Trivedi

An Evaluation Of Biofield Treatment On Thermal, Physical And Structural Properties Of Cadmium Powder, Mahendra Kumar Trivedi, Gopal Nayak, Shrikant Patil, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana

Mahendra Kumar Trivedi

In Vitro Evaluation Of Biofield Treatment On Cancer Biomarkers Involved In Endometrial And Prostate Cancer Cell Lines, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Biofield Treatment: An Alternative Approach To Combat Multidrug-Resistant Susceptibility Pattern Of Raoultella Ornithinolytica, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Characterization Of Physical And Structural Properties Of Brass Powder After Biofield Treatment, Mahendra Kumar Trivedi, Gopal Nayak, Rama Mohan Tallapragada, Omprakash Latiyal, Snehasis Jana

Mahendra Kumar Trivedi

Evaluation Of Biofield Modality On Viral Load Of Hepatitis B And C Viruses, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Study background: Nowadays, hepatitis is a major challenge for clinical research, regulatory bodies, and clinicians who are trying to assess the more effectiveness of antiviral therapy against patients. Viral load count is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of hepatitis. High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the impact of biofield modality on hepatitis B virus (HBV) and hepatitis C virus (HCV) in terms of viral load as surrogate marker.

Method: The …

Spectroscopic Characterization Of Chloramphenicol And Tetracycline: An Impact Of Biofield Treatment, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline. The present study was aimed to evaluate the impact of biofield treatment for spectroscopic characterization of chloramphenicol and tetracycline using FT-IR and UV-Vis spectroscopy. Methods:The study was performed in two groups (control and treatment) of each antibiotic. The control groups remained as untreated, and biofield treatment was given to treatment groups. Results: FT-IR spectrum of treated chloramphenicol exhibited the decrease in wavenumber of NO2 from 1521 cm-1 to 1512 cm-1 and increase in wavenumber of …

Influence Of Biofield Treatment On Physical, Structural And Spectral Properties Of Boron Nitride, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Boron nitride (BN) is known for high hardness, thermal stability, thermal conductivity, and catalytic action. The aim of this study was to investigate the effect of biofield treatment on physical, structural and spectral properties of BN powder. The control and treated sample of BN powder were characterized by X-ray diffraction (XRD), surface area analysis and Fourier transform infrared spectroscopy (FT-IR). XRD results indicated that biofield treatment had substantially changed the crystallinity of BN powder as compared to control. Apart from the crystallinity, significant changes were also observed in lattice parameter, density and molecular weight of the treated BN powder as …