Open Access. Powered by Scholars. Published by Universities.®

Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 4 of 4

Full-Text Articles in Biology

Growth, Sucrose Synthase, And Invertase Activities Of Developing Phaseolus Vulgaris L. Fruits, S. Sung, Wen-Jang Shieh, Donald Geiger, Clanton Black Jan 2015

Growth, Sucrose Synthase, And Invertase Activities Of Developing Phaseolus Vulgaris L. Fruits, S. Sung, Wen-Jang Shieh, Donald Geiger, Clanton Black

Donald R. Geiger

Activities of the sucrose-cleaving enzymes, acid and neutral invertase and sucrose synthase, were measured in pods and seeds of developing snap bean (Phaseolus vulgaris L.) fruits, and compared with 14C-import, elongation and dry weight accumulation. During the first 10 d post-anthesis, pods elongated rapidly with pod dry weight increase lagging behind by several days. The temporal patterns of acid invertase activity and import coincided closely during the first part of pod development, consonant with a central role for this enzyme in converting imported sucrose during pod elongation and early dry weight accumulation. Later, sucrose synthase became the predominant enzyme of …


Over-Expression Of Udp-Glucose Pyrophosphorylase In Hybrid Poplar Affects Carbon Allocation, Heather D. Coleman, Thomas Canam, Kyu-Young Kang, David D. Ellis, Shawn D. Mansfield Jan 2007

Over-Expression Of Udp-Glucose Pyrophosphorylase In Hybrid Poplar Affects Carbon Allocation, Heather D. Coleman, Thomas Canam, Kyu-Young Kang, David D. Ellis, Shawn D. Mansfield

Faculty Research & Creative Activity

The effects of the over-expression of the Acetobacter xylinum UDP-glucose pyrophosphorylase (UGPase) under the control of the tandem repeat Cauliflower Mosaic Virus promoter (2335S) on plant metabolism and growth were investigated in hybrid poplar (Populus alba3grandidentata). Transcript levels, enzyme activity, growth parameters, leaf morphology, structural and soluble carbohydrates, and soluble metabolite levels were quantified in both transgenic and wild-type trees. Transgenic 2335S::UGPase poplar showed impaired growth rates, displaying reduced height growth and stem diameter. Morphologically, 2335S::UGPase trees had elongated axial shoots, and leaves that were substantially smaller in size when compared with wild-type trees at equivalent developmental stages. Biochemical analysis …


Over-Expression Of Udp-Glucose Pyrophosphorylase In Hybrid Poplar Affects Carbon Allocation, Heather D. Coleman, Thomas Canam, Kyu-Young Kang, David D. Ellis, Shawn D. Mansfield Jan 2007

Over-Expression Of Udp-Glucose Pyrophosphorylase In Hybrid Poplar Affects Carbon Allocation, Heather D. Coleman, Thomas Canam, Kyu-Young Kang, David D. Ellis, Shawn D. Mansfield

Thomas Canam

The effects of the over-expression of the Acetobacter xylinum UDP-glucose pyrophosphorylase (UGPase) under the control of the tandem repeat Cauliflower Mosaic Virus promoter (2335S) on plant metabolism and growth were investigated in hybrid poplar (Populus alba3grandidentata). Transcript levels, enzyme activity, growth parameters, leaf morphology, structural and soluble carbohydrates, and soluble metabolite levels were quantified in both transgenic and wild-type trees. Transgenic 2335S::UGPase poplar showed impaired growth rates, displaying reduced height growth and stem diameter. Morphologically, 2335S::UGPase trees had elongated axial shoots, and leaves that were substantially smaller in size when compared with wild-type trees at equivalent developmental stages. Biochemical analysis …


Over-Expression Of Udp-Glucose Pyrophosphorylase In Hybrid Poplar Affects Carbon Allocation, Heather Coleman, Thomas Canam, Kyu-Young Kang, David Ellis, Shawn Mansfield Jan 2007

Over-Expression Of Udp-Glucose Pyrophosphorylase In Hybrid Poplar Affects Carbon Allocation, Heather Coleman, Thomas Canam, Kyu-Young Kang, David Ellis, Shawn Mansfield

Faculty Research & Creative Activity

The effects of the over-expression of the Acetobacter xylinum UDP-glucose pyrophosphorylase (UGPase) under the control of the tandem repeat Cauliflower Mosaic Virus promoter (2335S) on plant metabolism and growth were investigated in hybrid poplar (Populus alba3grandidentata). Transcript levels, enzyme activity, growth parameters, leaf morphology, structural and soluble carbohydrates, and soluble metabolite levels were quantified in both transgenic and wild-type trees. Transgenic 2335S::UGPase poplar showed impaired growth rates, displaying reduced height growth and stem diameter. Morphologically, 2335S::UGPase trees had elongated axial shoots, and leaves that were substantially smaller in size when compared with wild-type trees at equivalent developmental stages. Biochemical analysis …