Biology Commons^™

Extraction And Characterisation Of Essential Oils From Waste Eucalyptus Foliage, Hugh O'Reilly

Theses

Extraction procedures such as steam distillation, solvent and soxhlet extraction have been investigated to determine the most effective method of extracting essential oil from the foliage of a number of species of Eucalyptus cultivated in Co. Kerry. Steam distillation has been found to be the most effective method of extraction. The steam distillation extraction method has been thoroughly studied in order to determine the factors affecting yield and composition of the distillate. Alteration of the distillation time has been found to produce oil high in 1,8-cineole content, a requirement of medicinal quality oil. The choice of individual distillation still used …

Effects Of Sludge Application On Prairie Establishment, Brenda Joan Durbahn

Dissertations and Theses @ UNI

Native prairie in Iowa has all but disappeared since the development of agricultural land by European settlers over the past 165 years. Reconstructing prairie is one way to replace some of the acreage that was lost. A byproduct of settling an area is the generation of garbage and other wastes. Currently there is a surplus of sludge, the waste product of waste water treatment facilities. This material is usually disposed of in landfills, used on agricultural land or reclamation projects.

A small area of prairie was reconstructed on the top of a closed portion of the Black Hawk County Solid …

The Use Of Rapd To Detect Genetic Variation In Populations Of Liatris Aspera Michx., Duane Joseph Kitchen

Dissertations and Theses @ UNI

The tall grass prairie is one of the most degraded and fragmented ecosystems on earth. Interest in conserving, restoring, and reconstructing tallgrass prairie began early in the 1900s, and has increased dramatically since the 1960s. Early restoration and reconstruction efforts relied primarily on seed collected from prairie remnants near the planting site, but with the increase in prairie planting efforts came a greater demand for large quantities of affordable, viable seed of native prairie species. Following an increase in the use of cultivated varieties (cultivars) of seed, concerns over the loss of genetic integrity of local populations and low genetic …

Monitoring Results For Pfiesteria Piscidida And Pfiesteria-Like Organisms From Virginia Waters In 1998, Harold G. Marshall, David W. Seaborn, Jennifer Wolny

Biological Sciences Faculty Publications

Results of an extensive 1998 monitoring program for the presence of Pfiesteria-like organisms (PLO) in Virginia estuaries indicate these dinoflagellates are widely distributed in both the water column, and as cysts in the sediment, however Pfiesteria piscicida was not detected at this time. The highest concentrations of PLO were in estuaries along the Virginia shore line of the Potomac River, and in western Chesapeake Bay estuaries from the Little Wicomico River to the Rappahannock River. The most common PLO included Cryptoperidiniopsis sp. and Gymnodinium galatheanum. The lowest PLO concentrations were at ocean side locations. PLO were also …

Field Sampling And Necropsy Examination Of Fish, A. S. Kane, A. Baya, R. Reimschuessel, K. M. St. Pé, C. A. Poukish, C. P. Driscoll

Virginia Journal of Science

This paper presents an overview of observational and fish sampling tech­niques for investigating fish lesions, morbidity and mortality. These sam­pling techniques and investigations are much like detective work and require attention to detail, common sense, technical proficiency and experience. To solve the mystery of a fish kill, the investigator must use available evidence and clues to piece together a series of events that often have long since passed. The cause of these field events may be chemical, biological or physical; more often, it is some combination of these. An initial categorization approach may be used to reduce the great number …

Virginia's Pfiesteria Monitoring Program: Water Quality, Roger K. Everton, Wick T. Harlan, Jonathan W. Priest, Mark S. Alling

Virginia Journal of Science

During the summer and early fall of 1998, 34 estuarine stations in Virginia were sampled for Pfiesteria like organisms (PLOs) and associated water quality conditions. Stations were sampled either bimonthly (20 stations) or monthly (14 stations) from June to October 1998. At each station, a set of live and Lugol' s preserved samples were collected for presumptive counts of PLOs and water quality conditions were determined. Water quality parame­ters measured included standard field parameters, nutrients (total, dissolved, and particulate), chlorophyll a, and conventional water quality indicators. Although PLOs were relatively low in 1998 as compared to 1997, presump­tive PLO counts …

Foreward, Virginia Journal Of Science

Virginia Journal of Science

Foreward to the Winter 1999 issue of Virginia Journal of Science, discussing Pfiesteria Piscicida, written by Harold G. Marshall.

Distribution Of Desmin And Vimentin In The Rat Endometrium Duringearly Pregnancy, Emi̇n Türkay Korgun, Mevlüt Asar, Necdet Demi̇r, Ramazan Demi̇r

Turkish Journal of Biology

The aim of these studies is to analyze, by immunohistochemical methods, the emergence and distribution of vimentin and desmin in stromal cells of rat endometrium in early pregnancy and determine their role in decidualization and implantation biology. For this purpose totally 1-8 days pregnant 64 female and 32 male rats were used. On first day of pregnancy vimentin and third day of pregnancy desmin were staining respectively in the endometrial stromal tissue of rat. Although vimentin was seen during the early pregnancy, following the day 5 of pregnancy when the implantation begins interestingly desmin was only located in the antimezometrial …

Microhabitat Preferences Of Bullfrog Tadpoles (Rana Catesbeiana) Of Different Ages, Geoffrey R. Smith

Faculty Publications

No abstract provided.

"A Revision Of The Genus Platythelphusa A. Milne-Edwards, 1887 From Lake Tanganyika, East Africa" , Neil Cumberlidge, Richard V. Sternberg, I R. Bills, H A. Martin

Journal Articles

No abstract provided.

The Limits Of Specificity: An Experimental Analysis With Rna Aptamers To Ms2 Coat Protein Variants, Marc Spingola, Ichiro Hirao, David Peabody, Andrew Ellington

Biology Department Faculty Works

It has been hypothesized that selections for aptamers with high affinity for a given target molecule will of necessity identify aptamers that have high specificity for that target. We have attempted to assess this hypothesis by selecting aptamers that can bind to MS2 coat protein or to single- or double-substitution variants of the coat protein. Some aptamers selected to bind MS2 coat protein or its variants were mildly specific for their cognate targets, discriminating by two- to fourfold against closely related proteins. Specificity determinants on both the coat proteins and the aptamers could be identified. However, many aptamers could readily …

Characterization Of A Membrane Bound Proteolytic Enzyme Of Rat Osteoblasts, Anthony M. Orrico

Seton Hall University Dissertations and Theses (ETDs)

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Characterization Of The Adenovirus Protease Activity In Adenovirus Gene Therapy Preparations, Frederick William Porter Iv

Seton Hall University Dissertations and Theses (ETDs)

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Comparisons Of Plant Species Richness, Relative Abundance, And Diversity In Two Prairie Restorations And Two Remnant Prairie Preserves In Stearns County, Minnesota, Christopher J. Thompson

Honors Theses, 1963-2015

Prairies once covered vast areas of the Midwestern United States. In Minnesota, less than one percent of the state's original eighteen million acres of prairie remains. Restoration of prairie vegetation has become a popular means of augmenting preservation efforts and increasing the area of this rare native habitat. In this study I used species-area and rank abundance relationships to compare the species richness and diversity of restored prairies at St. John's University and Sand Prairie to remnant prairie preserves at Roscoe and Sand Prairie (all in Stearns County, Minnesota). I visually estimated relative abundance at each site using randomly placed …

The Use Of The Dna Comet Assay To Detect Genetic Damage In Rana Pipiens Taken From Affected Versus Non-Affected Sites, Lisa Marie Hood

Honors Theses, 1963-2015

Deformed frogs have been appearing frequently in Minnesota and throughout the United States. One hypothesis for the cause of deformities is genetic damage as a result of pesticides. This project will use the DNA Comet Assay to determine the extent of genetic damage in affected sites (sites with a greater number of deformities) versus non-affected sites (sites with fewer deformities). The DNA Comet Assay involves electrophoresis of cells in which the membranes have been solubilized and the DNA uncoiled. Electrophoresis spreads the genetic material. The length of the spread indicates the amount of genetic damage in the form of single …

Detection Of The Bacteroides Fragilis Group In Water By Nested Polymerase Chain Reaction, James Stuart Ferriss

Honors Theses

No abstract provided.

Limitation Of Algal Growth By Iron Deficiency In The Australian Subantarctic Region, Peter N. Sedwick, Giacomo R. Ditullio, David A. Hutchins, Philip W. Boyd, F. Brian Griffiths, A. Clive Crossley, Thomas W. Trull, Bernard Queguiner

OES Faculty Publications

In March 1998 we measured iron in the upper water column and conducted iron- and nutrient-enrichment bottle-incubation experiments in the open-ocean Subantarctic region southwest of Tasmania, Australia. In the Subtropical Convergence Zone (∼42°S, 142°E), silicic acid concentrations were low (< 1.5μM) in the upper water column, whereas pronounced vertical gradients in dissolved iron concentration (0.12-0.84 nM) were observed., presumably reflecting the interleaving of Subtropical and Subantarctic waters, and mineral aerosol input. Results of a bottle-incubation experiment performed at this location indicate that phytoplankton growth rates were limited by iron deficiency within the iron-poor layer of the euphotic zone. In the Subantarctic water mass (∼46.8°S, 142°E), low concentrations of dissolved iron (0.05-0.11nM) and silicic acid (< 1μM) were measured throughout the upper water column, and our experimental results indicate that algal growth was limited by iron deficiency. These observations suggest that availability of dissolved iron is a primary factor limiting phytoplankton growth over much of the Subantarctic Southern Ocean in the late summer and autumn.

Five Myofibrillar Lesion Types In Eccentrically Challenged, Unloaded Rat Adductor Longus Muscle—A Test Model, Joyce L. Thompson, Edward Michael Balog, Robert H. Fitts, Danny A. Riley

Biological Sciences Faculty Research and Publications

Sarcomere disruptions are observed in the adductor longus (AL) muscles following voluntary reloading of spaceflown and hindlimb suspension unloaded (HSU) rat, which resemble lesions in eccentrically challenged muscle. We devised and tested an eccentric contraction (ECCON) test system for the 14-day HSU rat AL. Six to 7 hours following ECCON, ALs were fixed to allow immunostaining and electron microscopy (EM). Toluidine blue-stained histology semithin sections were screened for lesion density (#/mm²). Serial semithin sections from the ECCON group were characterized for myosin immunointensity of lesions. Five myofibrillar lesion types were identified in histological semithin sections: focal contractions; wide …

Obtaining And Sampling Of High Quality Energetic-Protein Mixtures (Pottages) Of Tuber-Cereal-Leguminous Bases In The Candelaria Area, Maria Lilian Maldonado Chavez

Theses and Dissertations

Specially designed foodstuffs destined for children of weaning age in the community of Candelaria were developed and evaluated biologically. These victuals had as bases combinations of tubers-cereals-legumes of high nutritional quality to be implemented in the home. Based on the availability of food and alimentary habits of the region, seven mixtures were formulated of triples and quadruples containing tubers (potato, oca, papaliza, and isano); cereals (rice, wheat, corn, and quinua); and legumes (tarwi, broad bean, and peanut). The cereal-leguminous proportion was maintained as one complement and the addition of the tubers. These mixes were subjected to biological tests, obtaining incremental …

Investigation Of The Glucose-Induced Inactivation Of Maltose Permease In Saccharomyces, Igor L. Medintz

Dissertations, Theses, and Capstone Projects

The addition of glucose to maltose fermenting Saccharomyces cerevisiae cells results in the rapid loss of maltose transport activity. This results both from the repression of the maltose permease gene transcription and from the post-translational inactivation of maltose permease through a process termed glucose-induced inactivation of maltose permease. The inactivation consists of two separable processes, a rapid inhibition of maltose transport activity and by a slower degradation of maltose permease protein. Degradation is dependent on endocytosis, vesicular sorting and vacuolar proteolysis, and is independent of the proteasome. Furthermore, maltose permease exists in differentially phosphorylated forms.

Ubiquitin and the ubiquitin-conjugating system …

Cyclopropane Fatty Acid Expression In Plants, Katherine M. Schmid

Scholarship and Professional Work - LAS

Pants [sic] are transformed with a bacterial cyclopropane fatty acid synthase gene to produce lipids containing cyclopropane fatty acids. Using this technology dihydrosterculate is produced in oilseed crops such as rape.

Effects Of Lead On Inflammatory Microhemodynamics In Male Wistar Rats, Tanaz Mistry

Seton Hall University Dissertations and Theses (ETDs)

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Francis Harper Papers, Zach S. Henderson Library Special Collections

Finding Aids

This collection consists of the professional and personal papers of Cornell-trained naturalist Dr. Francis Harper. Materials span 1904-1972 and include field notebooks, correspondence, photographs, and audiovisual materials documenting the plant and animal life as well as human ecology of the Okefenokee Swamp area of South Georgia. The photographs within Series 1 show the flora, fauna, and people of the Okefenokee swamp. The photographs were taken by the Harpers while on their trips to the swamp. Photographs are all in black and white and range in size. The Field Journals chronicle his travels in the okefenokee, Canada, and New England. Materials …

Developing A Gene Specific Probe For Phosphoenolpyruvate Carboxykinase Mrna, Stephen M. Kuntz

Seton Hall University Dissertations and Theses (ETDs)

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Oviposition Preference And Offspring Performance Of Eurosta Solidaginis On Genotypes Of Solidago Altissima, Warren Abrahamson, T. Craig, J. Itami, J. Horner

Warren G. Abrahamson, II

No abstract provided.

Growing Pollen Tubes Possess A Constitutive Alkaline Band In The Clear Zone And A Growth-Dependent Acidic Tip, Joseph Kunkel, J, Feijo, J. Sainhas, G. Hackett, P. Hepler

Joseph Kunkel

Using both the proton selective vibrating electrode to probe the extracellular currents and ratiometric wide-field fluorescence microscopy with the indicator 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF)-dextran to image the intracellular pH, we have examined the distribution and activity of protons (H+) associated with pollen tube growth. The intracellular images reveal that lily pollen tubes possess a constitutive alkaline band at the base of the clear zone and an acidic domain at the extreme apex. The extracellular observations, in close agreement, show a proton influx at the extreme apex of the pollen tube and an efflux in the region that corresponds to the position of …

Episodic Reproduction In Two Fire-Prone Palms, Serenoa Repens And Sabal Etonia (Palmae), Warren Abrahamson

Warren G. Abrahamson, II

No abstract provided.

Influence Of Plant Genotype And Early-Season Water Deficits On Oviposition Preference And Offspring Performance In Eurosta Solidaginis (Diptera: Tephritidae), Warren Abrahamson, J. Horner

Warren G. Abrahamson, II

No abstract provided.

Host-Plant Genotype And Other Herbivores Influence Goldenrod Stem Galler Preference And Performance, Warren Abrahamson, J. Cronin

Warren G. Abrahamson, II

No abstract provided.