Biology Commons^™

A Gene Expression Profile Associated With Successful Cns Axon Regeneration In Xenopus Laevis, Jamie L. Belrose

Legacy Theses & Dissertations (2009 - 2024)

Background: The South African claw-toed frog, Xenopus laevis, is uniquely suited for studying differences between regenerative and non-regenerative responses to CNS injury within the same organism, because some CNS neurons (e.g., retinal ganglion cells after optic nerve crush (ONC)) regenerate axons throughout life, whereas others (e.g., hindbrain neurons after spinal cord injury (SCI)) lose this capacity as tadpoles metamorphose into frogs. Tissues from these CNS regions (frog ONC eye, tadpole SCI hindbrain, frog SCI hindbrain) were used in a three-way RNA-seq study of axotomized CNS axons to identify potential core gene expression programs for successful CNS axon regeneration.

Hormonal Responses To Seasonal Thermal And Ecological Stressors In Japanese Macaques (Macaca Fuscata), Lilianne Nelson

Masters Theses

Relative to most primate species, Japanese macaques (Macaca fuscata) experience extreme seasonal variation in thermal and ecological stressors that can impact energetic demands. To cope with these environmental changes, levels of metabolic hormones, such as cortisol and triiodothyronine, fluctuate to facilitate energetic adjustments. While previous research in primates has investigated thermal and ecological stressors individually, a combined assessment of these stressors alongside hormone levels can provide a more holistic understanding of the relationship between a primate’s thermoregulation, energetic balance, and stress. The goals of this study were to determine the effects of season, temperature and ecological stress on …

Discerning Competitive Vs. Facilitative Relationships Of Weeds On Forest Restoration Efforts At La Sal Del Rey, Tx, Mylen A. Arias

Theses and Dissertations

Dryland ecosystems need effective restoration strategies to address severe degradation. It is often assumed that voluntary forb weeds either compete with or have no effect on native seedlings. In contrast, theory and empirical work suggest a potential facilitative role for such forb weeds. We conducted a manipulative weed exclusion experiment at a semi-arid site in South Texas targeted for large scale forest restoration and subsequently dominated by early successional forb weeds to discern the net effect of these weeds on the growth and survival of target thornscrub tree and shrub seedlings. Overall, weed presence did not significantly affect seedling mortality …

Developing Methods To Survey, Collect, Process, And Screen Endemic Entomopathogenic Fungi Against The Asian Citrus Psyllid In The Lower Rio Grande Valley, Jonathan Cisneros

Theses and Dissertations

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), vectors “Candidatus Liberibacter spp.”, the causative agent of Citrus Greening Disease. Managing ACP is imperative given the continuous increase in HLB positive trees. An aspect of integrated pest management (IPM) is the use of entomopathogenic fungi for the biological control of D. citri.

In order to identify endemic entomopathogenic fungi actively infecting D. citri, ACP were collected from local residential areas, surface sterilized, and plated on a semi-selective agar medium. Collection of over 9,300 samples from 278 sites throughout the LRGV led to the positive identification of …

Disturbance, Defense And Fitness In Silverleaf Nightshade (Solanum Elaeagnifolium) In Their Native Range In South Texas, Jesus Chavana

Theses and Dissertations

The role of human disturbance in accelerating weed growth is well understood. While most of these studies have focused on soil mediated disturbance, mowing is also a management practice that could impact weed traits. Using silver leaf nightshade (Solanum elaeagnifolium), a noxious and invasive weed, we asked whether continuous mowing affects growth and plant defense traits. We found that mowed plants produced significantly less fruits, had lower total seed fitness, but had higher seed mass, and germinated significantly faster. When three common herbivores were allowed to feed on the seedlings; tobacco hornworm (Manduca sexta), a generalist Solanaceae caterpillar gained more …

Detecting Snake Fungal Disease (Ophidiomyces Ophiodiicola) In The Lower Rio Grande Valley, Manuel Zavala

Theses and Dissertations

Emerging diseases such as Snake Fungal Disease (SFD) caused by the fungus Ophidiomyces ophiodiicola (Oo) have caused population declines in various snake species in the United States which play a crucial role in the ecosystem as a natural pest control. This fungus targets the scales as a medium to thrive on which can lead to facial disfiguration and respiratory infections. We examined snakes to see if SFD was present in the LRGV and if other fungal species pose a threat to the various snake species population. The data for this study consisted of 14 live snakes captured in …

Fish And Macroinvertebrate Response To Restoration And Conservation Efforts, Madison C. Cogar

MSU Graduate Theses

Fish and macroinvertebrate response to restoration and conservation efforts varies in regards to the size and structure of the system (e.g. headwater streams in WV versus large rivers such as the Mississippi River). This project reviews fish and macroinvertebrate rebound in treated acid mine drainage (AMD) streams in WV as well as macroinvertebrate drift patterns in the Mississippi and Missouri Rivers. AMD is a product of a chemical reaction resulting in an acidic water outflow from mining sites, which may harm aquatic life. As a response, passive AMD treatment systems have been installed. I tested the effectiveness of remediation by …

The Effects Of White-Nose Syndrome On Cave-Dwelling Bats During Their Pre-Hibernation Season In Eastern Nebraska, Maggie Bockart

Theses/Capstones/Creative Projects

White-nose syndrome (WNS) is currently the leading cause of death to North American bats and was first documented in the United States in 2006 on the East Coast. Infection with the fungus Pseudogymnoascus destructans (Pd) causes bats to arise from hibernation with unnecessary frequency, leading to depletion of fat reserves that are imperative for extended periods of torpor. In October 2014, biologists surveyed the activity levels of bats at Fontenelle Forest in eastern Nebraska using acoustic detectors and determined what species of bats were present and their relative abundance before being exposed to WNS. By returning to the …

Analysis Of Seasonal Changes In Thermal Stress Resilience And Innate Immunity In The Temperate Coral, Astrangia Poculata, From Future Climate Impacts, Tyler Eugene Harman

Masters Theses

Over the years, global warming has had a devastating effect on coral reef ecosystems. Anthropogenic influences have caused significant increases in greenhouse gases, with a subsequent increase in solar radiation held within Earth’s atmosphere leading to increasing global temperatures. The increasing temperatures from concurrent increases in greenhouse gases impact fragile marine ecosystems such as coral reefs, which require particular environmental parameters such as temperature in order to survive and maintain a diverse ecosystem in which many marine species rely on. These increases in temperature exacerbate phenomena such as bleaching events and coral disease, drastically impacting coral on a global scale …

Tissue-Specific Responses From A Global Developmental Signal: A Detailed Subcellular Analysis Of The 20e And Rab Signaling Pathways Controlling Exocrine Secretion During Drosophila Development, Nichalas Nelson

UNLV Theses, Dissertations, Professional Papers, and Capstones

During metamorphosis, the steroid hormone 20-hydroxecydysone (20E) triggers a plethora of tissue-specific responses in the common fruit fly, Drosophila melanogaster. The salivary glands are exocrine organs that respond to a large pulse of 20E to secrete a massive cargo of stored glycoproteins called glue. Because the glands are amenable to ex vivo-organ culture and powerful genetic manipulations, they are an excellent model for studying the conserved developmental and physiological signaling pathways that regulate the general function of these specialized secretory tissues. Our lab has generated transgenic animals in which the secreted glue molecules are tagged with fluorescent proteins. I have …

Soil Response To Fire Frequency In The Northern Columbia Basin Sagebrush Steppe, Leslie C. Nichols

Boise State University Theses and Dissertations

Fire is one of the most significant disturbances in an ecosystem, as it is capable of altering the physical, chemical, and biological properties of soil, and the fire frequency in semi-arid ecosystems is increasing. These changes can potentially alter plant-soil feedbacks that may affect post-fire recovery of the native plant and soil communities and lead to an ecosystem state change. However, there is much uncertainty about the magnitude of change as soils are exposed to more fires, because soil recovery and changes in fire severity following a first fire mediate the impact of successive fires on soil properties. To improve …

Spatial Use By Mammals Within Two State Parks In The Ozarks National Scenic Riverways, Benjamin Aaron Smith

MSU Graduate Theses

A mammalian species inventory with comparisons between sampled sites was conducted via multiple methodologies to document presence of mammals at two Missouri state parks within the Ozark National Scenic Riverways. Camera traps, small mammal traps, acoustic detectors, and mist nets were used to detect species at the parks, and species similarity indices and occupancy analyses were used to discern use of space. A mammalian inventory was compiled for each area of inquiry. Greater diversity was found at the park with more variable habitat types. Bat activity was more in the park with a known hibernaculum, though species specific activity differed …

The Design And Validation Of A Qpcr-Based Approach To Cell Quantification Of Fungal-Algal Symbioses, Isabelle Katherine Garlotte

Honors Theses

Symbiotic relationships are extremely common in virtually all species. These relationships between organisms, particularly mutualistic symbioses, can have an incredibly large impact and can greatly affect the evolution of the species involved. Of particular interest is the suspected mutualistic relationship between green algae and fungi that may hold key evidence of how terrestrial plants evolved. In this experiment we established a method of quantifying the cells of each species involved in these symbioses through qPCR analysis to determine if the relationships are truly mutualistic. We developed a system for using DNA copies as a proxy for cell count in co-cultures …

On The Straight And Narrow: How Cultural Beliefs About Sex/Gender Manifest In College Biology Learning Environments., Katherine Ray Kng

Electronic Theses and Dissertations

This dissertation focused on the ways that social beliefs about sex/gender and sexuality manifest in biology classrooms. Especially for health science students, classes include topics like sex, sexuality, sexual behaviors, or sex determination that forms the foundation of their understanding of human forms and behaviors. If the information they receive does not include the LGBTQIA+ community, their education will fail to adequately prepare them for the reality of healthcare practice where they will be expected to treat all patients with dignity and respect. There are three chapters in this dissertation. In chapter one, I asked what beliefs about and conceptions …

Modeling Neurofibromatosis Type Ii In Zebrafish, Hannah Nuszen

University Honors Program Senior Projects

Neurofibromatosis Type 2 (NF2) is a rare genetic disorder, classified as a neurocutaneous syndrome inherited through an autosomal dominant genetic mutation or deletion of the NF2 gene in humans. The tumor suppressor gene NF2 encodes the cytoskeletal protein MERLIN to promote apoptosis and inhibit abnormal cellular proliferation. Mutations in NF2 lead to a deficiency of functional MERLIN in humans and can result in hearing loss, disability, and premature death. These symptoms are due to uncontrollable growth of schwann cells generating bilateral benign tumors (schwannomas) within the nerve sheath of cranial nerve VIII. Here we seek to understand the function of …

Undergraduate Students Use Moral Reasoning And Belief In Genetic Determinism In Response To A Crispr/Cas9 Socioscientific Issue., Katie M. Seiter

Electronic Theses and Dissertations

This dissertation explores how students reason about genetic engineering socioscientific issues (SSIs) related to a recently developed, powerful genome editing technology called CRISPR/Cas9. It is divided into three chapters. Chapter One describes an exploratory study that characterized students’ moral reasoning using a sociocultural theoretical framework. I used content analysis and logistic regression to investigate how academic and social factors influenced moral reasoning. Students generally opposed the use of CRISPR/Cas9 technology for non-medical enhancements, and the moral considerations used were influenced by genetics knowledge level and demographic variables such as gender and socio-economic status. Further investigation of moral perspectives for students …

Assessment Of Genetic Diversity In Largetooth Sawfish, Pristis Pristis, Populations Over The 20th Century, Ann Fearing

Master's Theses

The Critically Endangered Largetooth Sawfish, Pristis pristis, experienced global declines in range and abundance over the past century and Australia is now their last stronghold. This research aimed to understand whether these declines have been accompanied by a reduction in levels of genetic diversity. Using P. pristis tissue samples sourced from natural history specimens, three fragments (616-bp, 386-bp, 141-bp) in the mitochondrial DNA (mtDNA) control region were PCR-amplified. At each fragment, significant genetic structure was found in P. pristis overall (Φ_ST=0.946, N=9; 0.813, N=54; 0.771, N=99). The Indo-West Pacific and Atlantic/Eastern Pacific each harbor genetically distinct lineages of …

Microbe-Mineral Interactions During Exceptional Fossil Preservation, Stromatolite Formation, And Desert Varnish Growth, Michael Strange

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Ediacaran to Cambrian Deep Spring Formation consists of mixed carbonate-siliciclastic strata which contain an increasingly complex and biogeographically important biota. Past investigations of the Deep Spring Formation at Mt. Dunfee, Nevada, explored the highly diverse microbialite reefs consisting of a wide range of stromatolite morphologies which exerted significant control on local sedimentation and topography. Early investigations also documented the biomineralizing metazoan Cloudina (an Ediacaran index fossil). However, recent exploration of the area has resulted in the discovery of several new metazoan fossil communities consisting of a diverse assemblage of Ediacaran soft-tissue tubicolous vermiforms (tube fossils) similar to Cloudina. The …

Role Of Herbivore Associated Elicitors In Plant Defense Signaling, Akanksha Gandhi

Theses and Dissertations

Chapter 2: In this chapter, I have provided an update on recent developments and advances on early signaling events in plant-herbivore interactions, with a particular emphasis on the membrane potential changes (Vm), calcium (Ca2+), reactive oxygen species (ROS) and plant ion channels involvement in early signaling events initiation and propagation of defense signaling cascade.

Chapter 3: In this chapter, we show that tobacco hornworm (Manduca sexta) caterpillar oral secretion (OS) induces ROS in tomato (Solanum lycopersicum) protoplasts. By using a dye-based ROS imaging approach, our study showed that the application of Plant-Fed (PF) M. sexta OS …

Interactive Effects Of Heat Stress And Pesticides Co-Exposure On Swimming Behavior, Oxidative Stress, Antioxidant Expression, And Redox Status In Common Goldfish (Carassius Auratus), Brittney Danielle Lacy

Theses and Dissertations

Aquatic ecosystems are inundated by environmental pesticides. This study, observed the dose-dependent effects of pesticides cocktail (metalachlor, linuron, isoproturon, tebucanazole, aclonifen, atrazine, pendimethalin, and azinphos-methyl) and elevated temperature (32 °C for 4-week exposure) on morphology of gills and kidneys, and expression of nitrotyrosine protein (NTP), dinitrophenyl protein (DNP), catalase (CAT), superoxidase dismutase (SOD), Na+/K+-ATPase, renin, and apoptosis in tissues of goldfish. Additionally, the effects on the free-swimming behavior were also observed. Histological analysis showed wide-spread damage in tissues at higher temperature and pesticides co-exposure. Immunohistochemical analysis demonstrated alterations in NTP, DNP, CAT and SOD expressions in tissues at higher temperature …

Role Of Glycolytic Metabolism In Glioblastoma Multiforme, Shreya Udawant

Theses and Dissertations

PI3K/AKT/mTOR signaling pathway is activated in 90% of all Glioblastoma Multiforme (GBM) tumors. We used a dual targeting approach to interfere with the phosphoinositide 3-kinase- mammalian target of rapamycin (PI3K-mTOR) pathway by treating U87MG GBM cells with NVP-BEZ235 (PI3K and mTOR a dual inhibitor) and, their downstream effects on molecular regulation on glycolytic genes were investigated using RNA-sequencing and gene set enrichment analysis (GSEA). RNA-seq identified 7803 differentially regulated genes in response to NVP-BEZ235. GSEA identified two glycolysis-related gene sets had significant differences (P < 0.05) in gene expression between control and NVP-BEZ235 treated samples. The GSEA core enrichment reported 17 and 35 genes were significantly downregulated (P adj < 0.05) in response to NVP-BEZ235 treatment in two different glycolysis related gene sets. We validated the expression of genes using qRT-PCR in U87MG cells as well as other GBM cell lines. Our results suggest novel insights on hallmark metabolic reprogramming associated with the PI3K-mTOR inhibition and provides advances in developing molecular targeted therapies.

Assessing The Effects Of Cold Storage Regimes Of North American Bombus Queens: Long Live The Humble Queen, Thuy - Tien Thai Lindsay

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bumble bees (Bombus) provide critical pollination services for wild plants and crops. They are generalist pollinators, and do not depend on one singular floral type. Native bumble bees are adapted to pollinating many native plants, such as blueberries, squash, and tomatoes. A critical component of bumble bee pollination in the greenhouse tomato industry is the domestication of commercially bred colonies. However, the domestication of Bombus is pretty early in its development, and not all aspects of the bumble bee life cycle is fully understood. This thesis addresses one of the major obstacles in domestication, which is successful mimicking …

The Role Of The Recently Deorphanized G-Protein Coupled Receptor, Gpr171, In Morphine Tolerance And Withdrawal, Leela Afrose

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Opioid analgesics, such as morphine, represent the gold standard pain killer and the most frequently used drugs for the treatment of moderate to severe pain. Despite being a potent analgesic, morphine has unwanted and dangerous side effects with repeated use, such as tolerance and withdrawal. Tolerance is a state when a person no longer responds to a drug and a higher dose is required to achieve the same initial pain relief. Withdrawal is a set of undesirable psychological and physiological symptoms that occur after someone stops taking a drug or reduces the dose. Morphine tolerance and withdrawal play a vital …

Coral Bleaching: A Global Analysis Of Bright Spots, Shannon Elizabeth Sully

Theses and Dissertations

Thermal-stress events associated with climate change cause coral bleaching and mortality that threatens coral reefs globally. Yet coral bleaching and coral cover patterns vary spatially and temporally, and few studies have identified bright spots where corals have the potential to survive through climate change. This study synthesizes over 10,000 field observations globally of coral bleaching and coral cover from 1997to 2017 and uses a suite of environmental and temperature metrics to analyze coral bleaching and coral cover patterns. The objectives of this dissertation are to(1) identify the environmental variables that constitute bright spots for coral reefs, and (2) identify where …

Study Of Plants Used Against Infections By California Native American Tribes, Maria J. Rojas

Master's Theses

The objectives of this research were to evaluate the antibacterial activity and to determine the chemical composition of a list of medicinal plants used by Native Americans in California. Artemisia californica, Mimulus aurantiacus, Equisetum telmateia, Equisetum hyemale, and Marah fabacea were selected from a list of plants reported as having been used for ailments related to infections by tribes located in California. The extracts obtained through steam distillation from E. telmateia, E. hyemale and M. fabacea were assayed for in vitro antibacterial activity against 16 Gram-negative and 6 Gram-positive bacteria using disk diffusion assays and measuring the diameters of inhibition …

The Effects Of Multiple Stressors On Stream Communities: The Convergence Of Drought, Nutrient Pollution, And Invasive Species, Robert Joseph Fournier Iii

Graduate Theses and Dissertations

Freshwater systems experience multi-faceted degradation from a variety of ecological and environmental stressors. Three common stressors in these systems, drought, nutrient pollution, and invasive species, have wide-ranging effects on stream population- community- and ecosystem dynamics. We have a broad understanding of how each of these stressors works to influence stream systems independently. However, we still know relatively little about if, and how, these stressors might interact when they co-occur. Though drought is a natural part of many stream systems, all three of these stressors can be exacerbated or facilitated by anthropogenic actions. Accordingly, as human population and resource use continue …

Neogene And Quaternary Events Shaped Diversification And Speciation In Bhutanese Rheophilic Fishes Of The Family Nemacheilidae (Cypriniformes) And Sisoridae (Siluriformes), Karma Wangchuk

Graduate Theses and Dissertations

Biogeography of the Himalayan region [to include the Qinghai-Tibetan Plateau (QTP)] evolved over a ~30M year span, catalyzed by the collision of the Indian and Eurasian plates. The resulting uplift produced major ecological and climatic effects, that in turn drove the diversification of biodiversity. As a result, the QTP is designated as a global biodiversity hotspot particularly vulnerable to cumulative climatic effects, including shrinking distributions, declining numbers, and local extinctions. Understanding how the biodiversity within the Himalaya/ QTP was established and maintained is a necessary first step in prioritizing conservation efforts.

Fishes in global montane regions, such as the Himalaya, …

Effects Of Long-Term Variation In Temperature On Reproductive Phenology In A Population Of Eastern Bluebirds (Sialia Sialis), Paul Pleiman

Graduate Theses and Dissertations

This study investigates the relationship between multiple temperature variables, to include annual and pre-lay date temperatures with first-egg and mean first-egg lay dates of the eastern bluebird at the Warner Parks in Nashville, Tennessee, USA. Data is collected by citizen scientists for the Eastern Bluebird Nesting Box Project while visiting artificial nest boxes throughout the park and recording observations made during the breeding season. Temperature data is retrieved from the Northwest Alliance for Computational Science and Engineering’s Parameter-elevation Regressions on Independent Slopes Model (PRISM) Climate Group, based at Oregon State University. The analyses showed no correlation between annual or pre-lay …

Global Conservation Status Of Croaker And Drum (Family: Sciaenidae) And Role Of The Maw Trade, Claire E. Gorman

Biological Sciences Theses & Dissertations

Understanding threats and drivers of extinction risk is critical to conserving marine biodiversity. Although marine bony fishes are some of the most diverse and abundant vertebrates in the world, overexploitation is the major threat to these taxa. Species in the family Sciaenidae are important marine resources in many parts of the world, supporting small- and large-scale fisheries for consumption and the international maw (dried swimbladder) trade. Sciaenids exhibit a diverse array of life history characteristics, such as high fecundity, large body size, formation of spawning aggregations, and longevity. Some characteristics increase their susceptibility to anthropogenic stressors. Despite being ecologically and …

Assessment Of Factors Influencing Migratory Landbird Use Of Forested Stopover Sites Along The Delmarva Peninsula During Autumn Migration, J. Andrew Arnold

Biological Sciences Theses & Dissertations

Autumn migration is a time when billions of birds move from breeding grounds in North America to wintering grounds in Central and South America, with many individuals relying on stopover habitats en route for resting and refueling purposes. These stopover sites are critical to the survival of the hundreds of species of migratory landbirds that migrate annually, and thus identifying important stopover sites is a high priority for conserving such taxa. The Delmarva Peninsula; a coastal region of Delaware, Maryland, and Virginia along the mid-Atlantic flyway; consists of forested habitats with ample food and shelter that likely serves as quality …