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Full-Text Articles in Biology

Plant Growth Promotion By Bradyrhizobium Japonicum Under Heavy Metal Stress, M. Seneviratne, S. Gunaratne, T. Bandara, L. Weerasundara, Nishanta Rajakaruna, G. Seneviratne, M. Vithanage Jul 2016

Plant Growth Promotion By Bradyrhizobium Japonicum Under Heavy Metal Stress, M. Seneviratne, S. Gunaratne, T. Bandara, L. Weerasundara, Nishanta Rajakaruna, G. Seneviratne, M. Vithanage

Biological Sciences

The increase in usage of heavy metals in different industrial activities causes their existence in effluents. Excessive concentrations of these heavy metals pollute soil and water. Heavy metals cause toxicities and other harmful effects not only in humans and animals but also in plants and soil microorganisms. Heavy metals disrupt many biochemical and physiological activities in bacteria, including growth, development, enzyme and hormone production. Indole acetic acid (IAA) is one of the most important hormones in plants, which is secreted by both bacteria and plants. The present study assessed the effects of Ni, Pb and Cu on the growth of …


Limestone Flora Of The Simonton Corner Quarry Preserve, Rockport, Maine, Usa, Margaret Stern, Ian D. Medeiros, Luka Negoita, N. Rajakaruna May 2016

Limestone Flora Of The Simonton Corner Quarry Preserve, Rockport, Maine, Usa, Margaret Stern, Ian D. Medeiros, Luka Negoita, N. Rajakaruna

Biological Sciences

Limestone is a distinctive substrate that has significant effects on soils and plants. The present study characterizes the diversity of vascular plants, bryophytes, and lichens at the Simonton Corner Quarry Preserve, an abandoned limestone quarry in Rockport, Maine, USA, which was in operation in the late 1800s. We document vascular plant diversity and associated edaphic features (i.e., soil pH and elemental chemistry) using 30 535 meter plots spread throughout the site. For vascular plants, 114 species in 96 genera and 50 families were observed; few of these species are known to prefer calcareous environments, and 38% are nonnative. Conversely, the …


Trinucleotide Heterogeneity Expansions: In Vitro Molecule Amplification & Purification, Gennaro Dellicarpini May 2016

Trinucleotide Heterogeneity Expansions: In Vitro Molecule Amplification & Purification, Gennaro Dellicarpini

Biological Sciences

Simple sequence repeats (SSRs) are mutation-prone DNA tracts composed of tandem repetitions of relatively short motifs (Kashi & King, 2006). SSRs are found scattered throughout the human genome in both coding and non-coding regions. Present under the category of SSRs are Trinucleotide repeats, which may vary in number and, when expanded upon, provide the basis of at least nine neurodegenerative diseases. Large degrees of structural heterogeneity are found in CAG trinucleotide repeat DNA. DNA slippage leads these hetero-structures to form, since certain repeats slip out of the origin of replication and are carried into the next generation of replication. This …


Is The Effect Of Glucose On Hippocampal Memory Insulin-Dependent?, Roi Ankawa May 2016

Is The Effect Of Glucose On Hippocampal Memory Insulin-Dependent?, Roi Ankawa

Biological Sciences

Insulin is now established as a key regulator of brain mechanisms that include both glucose metabolism and synaptic plasticity, especially within the hippocampus. However, the complex set of signaling cascades mediating these effects is not yet understood. Recent studies, many from our lab, have established that insulin plays multiple roles in the brain: in addition to regulation of energy supply, metabolism, and feeding, our work has shown that hippocampal insulin is a key modulator of learning and memory. Exogneous insulin enhances, while pharmacological blockade of intrahippocampal insulin impairs, both metabolism and cognition. Moreover, when systemic insulin signalling is impaired, such …


Changes In Expression Of Innervation And Accompanying Epithelium Within Sjögren's Syndrome Nod/Shiltj Mouse Model, Nicholas Pagendarm May 2016

Changes In Expression Of Innervation And Accompanying Epithelium Within Sjögren's Syndrome Nod/Shiltj Mouse Model, Nicholas Pagendarm

Biological Sciences

The salivary gland produces saliva that aids in digestion and in maintaining homeostasis in the oral cavity. Saliva production is disrupted in Sjögren’s Syndrome (SS), where the salivary gland is attacked by the immune system, leading to loss of function and hyposalivation. SS is thought to be accompanied by changes in the salivary epithelium, which are incompletely understood. In this study we have used the non-obese diabetic (NOD) mouse as a model for SS to study the changes in salivary gland epithelium, as these models present autoantibodies and other characteristics such as immune infiltrates similar to those found in SS …


Fluorescence Analysis Of Expanded Trinucleotide Repeat Heterogeneity After In Vitro Pcr Amplification, Kidane M. Tewolde May 2016

Fluorescence Analysis Of Expanded Trinucleotide Repeat Heterogeneity After In Vitro Pcr Amplification, Kidane M. Tewolde

Biological Sciences

The expansion of trinucleotide repeats in human genomic DNA manifests into multiple neurodegenerative diseases (Amrane et al., 2005). At least nine human diseases stem from the expression of expanded trinucleotide repeats (Box, 2007). Simple sequences such as, 5’CAG/CTG’3 repeats, contain a potential pathogenicity once expanded past their original lengths; which is exemplified in Huntington’s Diseases and Myotonic Dystrophy. Once expanded CAG repeats are transcribed into RNA, these transcripts are translated, and the mal functioning proteins can lead to severe cell damage. Furthermore, the repeats can be passed down through generations, and in each germline, continuously expanding, gaining more repeated units …


Phenotypic Characterization Of Adipose-Specific Vdr Knockout Mice, Joseph D'Angelo May 2016

Phenotypic Characterization Of Adipose-Specific Vdr Knockout Mice, Joseph D'Angelo

Biological Sciences

Breast cancer is a prominent and lethal disease that currently affects close to three million people in the United States. Each year displays close to 250,000 new cases of breast cancer and around 40,000 deaths in the United States alone. A geographic pattern of breast cancer suggests a higher incidence in more temperate regions. Research has suggested that low Vitamin D levels associated with reduced sun exposure might contribute to increased breast cancer incidence. In recent studies, Vitamin D has been shown to slow the proliferation of breast cancer cells, but the mechanisms involved in vivo are poorly defined. It …


The Essential Oil Of Lippia Alba Affects Drosophila Behavior And Physiology, Jibin Manimala May 2016

The Essential Oil Of Lippia Alba Affects Drosophila Behavior And Physiology, Jibin Manimala

Biological Sciences

Lippia alba (LA) is a flowering shrub native to Central and South America. Its essential oil has been used in herbal medicine as an anti-anxiety drug and in aquaculture; it is used to sedate fish during transport (Daniel 2014. Essential oils are derived from plants usually through steam distillation. They are labeled as “essential” because it contains the odor of the plant. Its physiological action is unknown. We use the fruitfly Drosophila melanogaster to further define its behavioral actions and determine its underlying physiological effects. We first tested whether Lippia alba produced had an anesthetic effect on the fruitfly. We …


Neuronal Glutamate Transporter Eaac1 Regulates Motor Activity And Anxiety In Mice, Kelsey Fleming May 2016

Neuronal Glutamate Transporter Eaac1 Regulates Motor Activity And Anxiety In Mice, Kelsey Fleming

Biological Sciences

Obsessive Compulsive disorder (OCD) is a neuropsychiatric disorder characterized by the onset of recurrent thoughts, anxiety, and repetitive motor behaviors. The molecular basis of OCD remains elusive, but recent meta-analysis and genome-wide association studies (GWAS) suggest the existence of a genetic association between polymorphisms in the gene coding for excitatory amino acid carrier 1 (EAAC1) and OCD. It is also known that the Cortico-Striatal-Thalamo-Cortical (CSTC) pathway shows patterned hyperactivity in patients with OCD. EAAC1 is the primary neuronal glutamate transporter in the brain and is abundantly expressed in the cortex and the striatum, two regions that are part of the …


Solidago Gigantea Plants From Nonnative Ranges Compensate More In Response To Damage Than Plants From The Native Range, Huixuan Liao, Priscila Gurgel, Robert Pal, Ragan Callaway Jan 2016

Solidago Gigantea Plants From Nonnative Ranges Compensate More In Response To Damage Than Plants From The Native Range, Huixuan Liao, Priscila Gurgel, Robert Pal, Ragan Callaway

Biological Sciences

Resistance and tolerance are two ways that plants cope with herbivory. Tolerance, the ability of a plant to regrow or reproduce after being consumed, has been studied less than resistance, but this trait varies widely among species and has considerable potential to affect the ecology of plant species. One particular aspect of tolerance, compensatory responses, can evolve rapidly in plant species; providing insight into interactions between consumers and plants. However, compensation by invasive species has rarely been explored. We compared compensatory responses to the effects of simulated herbivory expressed by plants from seven Solidago gigantea populations from the native North …