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Analysis Of Sry Duplications On The Rattus Norvegicus Y-Chromosome, Jeremy W. Prokop, Adam C. Underwood, Monte E. Turner, Nic Miller, Dawn Pietzrak, Sarah Scott, Chris Smith, Amy Milsted
Analysis Of Sry Duplications On The Rattus Norvegicus Y-Chromosome, Jeremy W. Prokop, Adam C. Underwood, Monte E. Turner, Nic Miller, Dawn Pietzrak, Sarah Scott, Chris Smith, Amy Milsted
Amy Milsted
Background: Gene copy number variation plays a large role in the evolution of genomes. In Rattus norvegicus and other rodent species, the Y-chromosome has accumulated multiple copies of Sry loci. These copy number variations have been previously linked with changes in phenotype of animal models such as the spontaneously hypertensive rat (SHR). This study characterizes the Y-chromosome in the Sry region of Rattus norvegicus, while addressing functional variations seen in the Sry protein products. Results: Eleven Sry loci have been identified in the SHR with one (nonHMG Sry) containing a frame shift mutation. The nonHMGSry is found and conserved in …
Testosterone Effects On Renal Norepinephrine Content And Release In Rats With Different Y Chromosomes, Thomas J. Jones, Gail Dunphy, Amy Milsted, Daniel Ely
Testosterone Effects On Renal Norepinephrine Content And Release In Rats With Different Y Chromosomes, Thomas J. Jones, Gail Dunphy, Amy Milsted, Daniel Ely
Amy Milsted
The Y chromosome in spontaneously hypertensive rats (SHR) and stroke-prone rats has been shown to contain a locus that contributes to the hypertensive effect; both the sympathetic nervous system and testosterone may be involved. The objective of this study was to look at the effects of testosterone on renal norepinephrine (NE) release and content in the isolated perfused kidney in different Y chromosome backgrounds. The study involved male SHR, Wistar-Kyoto rats (WKY), and 2 consomic strains with different Y chromosomes (n=5 to 8 per group). Adult animals were castrated, and implants containing testosterone propionate were placed at the base of …
Amino Acid Function And Docking Site Prediction Through Combining Disease Variants, Structure Alignments, Sequence Alignments, And Molecular Dynamics: A Study Of The Hmg Domain, Jeremy W. Prokop, Thomas C. Leeper, Zhong-Hui Duan, Amy Milsted
Amino Acid Function And Docking Site Prediction Through Combining Disease Variants, Structure Alignments, Sequence Alignments, And Molecular Dynamics: A Study Of The Hmg Domain, Jeremy W. Prokop, Thomas C. Leeper, Zhong-Hui Duan, Amy Milsted
Amy Milsted
Background: The DNA binding domain of HMG proteins is known to be important in many diseases, with the Sox sub-family of HMG proteins of particular significance. Numerous natural variants in HMG proteins are associated with disease phenotypes. Integrating these natural variants, molecular dynamic simulations of DNA interaction and sequence and structure alignments give detailed molecular knowledge of potential amino acid function such as DNA or protein interaction. Results: A total of 33 amino acids in HMG proteins are known to have natural variants in diseases. Eight of these amino acids are normally conserved in human HMG proteins and 27 are …