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Full-Text Articles in Biology
Two-Photon Microscopy For Biomedical Studies, Yassel Acosta
Two-Photon Microscopy For Biomedical Studies, Yassel Acosta
Open Access Theses & Dissertations
Fluorescence optical microscopy is one of the most important tools to investigate biological samples and to observe structures in living cells. Based on the principle of the simultaneous absorption of two-photons and subsequent emission of fluorescent light we have developed a Two-Photon Laser Scanning Fluorescence Microscope that allow us to perform optical sectioning and three-dimensional reconstructions of structures in biological samples such as dopaminergic clusters of neurons and tracheal branches in Drosophila brain. The spatial confinement of the excitation light to the focal plane combined with the temporal compression of photons into short packets during femtosecond pulses make our system …
Spingolipid Metabolism In A Reptillian Protozoan, Entamoeba Invadens, Duran Dogan Debons
Spingolipid Metabolism In A Reptillian Protozoan, Entamoeba Invadens, Duran Dogan Debons
Open Access Theses & Dissertations
Entamoeba invadens (hereinafter E. invadens) is an anaerobic parasitic protozoan that can be found in water and soil. Although the host organisms for this parasite are snakes, lizards, and other reptile species, the parasite is morphologically identical to Entamoeba histolytica, a causative agent of human amebiasis. Entamoeba inavdens exists in two morphological forms--(1) a replicative trophozoite, and (2) a relatively dormant cyst. While the transformation of cyst to trophozoites (called "excystation") takes place in the stomach, the differentiation of trophozoites to cyst (known as "encystation"), occurs in the small intestine. Unlike the cyst, a trophozoite is unable to survive …