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Full-Text Articles in Other Biochemistry, Biophysics, and Structural Biology

Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera Jan 2017

Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera

Honors Undergraduate Theses

In this thesis, we investigate biological molecules on a micron scale in the ultraviolet spectral region through the non-destructive confocal absorption microscopy. The setup involves a combination of confocal microscope with a UV light excitation beam to measure the optical absorption spectra with spatial resolution of 1.4 μm in the lateral and 3.6 μm in the axial direction. Confocal absorption microscopy has the benefits of requiring no labels and only low light intensity for excitation while providing a strong signal from the contrast generated by the attenuation of propagating light due to absorption. This enables spatially resolved measurements of single …


Methane Fluxes And Porewater Dissolved Organic Carbon Dynamics From Different Peatlands Types In The Pastaza-Marañon Basin Of The Peruvian Amazon., Jhon Del Aguila-Pasquel Jan 2017

Methane Fluxes And Porewater Dissolved Organic Carbon Dynamics From Different Peatlands Types In The Pastaza-Marañon Basin Of The Peruvian Amazon., Jhon Del Aguila-Pasquel

Dissertations, Master's Theses and Master's Reports

Relevant components of the carbon budget such as methane fluxes and porewater dissolved organic carbon (DOC) are poorly characterized in the Peruvian Pastaza Marañon basin (PMB). PMB is associated with a tropospheric hotspot for methane and therefore it may represent a significant methane source. We aimed to quantify the magnitude of CH4 fluxes and porewater DOC; and to determine their predictors across different peatland types in the PMB (open peatland, palm swamp, and pole forest). The study was conducted in two peatland sites covering the three main vegetation types in PMB. We collected gas and water samples using chamber-based …