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Expression Analysis Of A Glucose Oxidase Transgene In Tobacco, Jordan Burt
Expression Analysis Of A Glucose Oxidase Transgene In Tobacco, Jordan Burt
Honors Theses
Glucose oxidase (GOX) is an enzyme in plants that catalyzes the oxidation of glucose to hydrogen peroxide and Glucono delta-lactone[5]. We have expressed GOX under the control of an estrogen inducible system, XVE, to analyze the gene's expression under this system compared to a system using the 35s system. The 35s system contains a promoter that constitutively turns on the GOX gene in the Nicotiana tabacum plant which causes the gene to always be turned on. Leaf disc assays were performed with discs from 35s, XVE, and also wild type plants (not containing the GOX gene) in order to extract …